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Composite microbial agent capable of improving soil fertility, and preparation method and application thereof

A technology of compound biological bacteria and soil fertility, applied in the field of compound biological bacteria agent, can solve problems such as soil micro-ecological environment imbalance, soil structure damage, waste of resources, etc., to reduce soil salinization, increase crop yield, and reduce waste of resources Effect

Pending Publication Date: 2018-01-26
黄山中科新佳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The long-term and large-scale use of chemical fertilizers leads to the destruction of soil structure, the decline of soil organic matter, and the imbalance of soil micro-ecological environment, which in turn leads to the decline of soil fertility, aggravation of plant diseases and insect pests, soil acidification and salinization
Excessive chemical fertilizers, except that some of them enter the lake through rainwater and cause eutrophication, most of them are fixed in the soil and difficult to be used by plants, resulting in a waste of resources, and the single chemical fertilizer composition often leads to a decline in crop quality

Method used

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  • Composite microbial agent capable of improving soil fertility, and preparation method and application thereof
  • Composite microbial agent capable of improving soil fertility, and preparation method and application thereof
  • Composite microbial agent capable of improving soil fertility, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of Bacillus megaterium fermentation broth: The formula of the culture broth is: tryptone 10g, yeast extract 5g, NaCl 10g, MnSO 4 -H 2 O 0.02g, water 1000mL, pH7.0-7.2. Sterilize the culture solution at 121°C for 20 minutes, inoculate the seed solution of Bacillus megaterium (Phospholyticum P) after cooling, the inoculation amount is 5%-10%, the culture temperature is 35-37°C, the rotation speed is 200rpm, and the culture time is 36-48 hours . The preparation and sterilization process of the seed culture medium is the same as that of the fermentation medium.

[0038] The preparation of Paenibacillus colloidus fermented liquid: the formula of culture liquid is: glucose 10.0g, (NH 4 ) SO 4 1g, K 2 HPO 4 2g, CaCO 3 0.5g, yeast powder 0.5g, NaCl 0.1g, MgSO 4 -7H 2 O 0.5g, water 1000mL, pH7.0-7.2. Sterilize the culture solution at 115°C for 15 minutes, inoculate Paenibacillus colioids (potassium solubilizer K) seed liquid after cooling, the inoculation...

Embodiment 2

[0042] Example 2 Phosphorus and potassium decomposing ability of compound bacterial liquid

[0043] Centrifuge the composite bacterial solution of Example 1, remove the culture medium, then resuspend it with sterile water to make a bacterial suspension, and insert the organic phosphorus (2g lecithin) respectively according to the inoculum size of 1% (v / v). , inorganic phosphorus (5g tricalcium phosphate), mineral powder (10g mica powder) liquid culture medium, the compound bacterial liquid that control group connects inactivation, place on shaker after inoculation and cultivate at 30 ℃, 200rpm vibration. Decomposition of phosphorus in the solution was measured after 12 days, and potassium decomposition in the solution was measured after 20 days. The determination method of water-soluble phosphorus was molybdenum-antimony anti-colorimetry, and the determination method of water-soluble potassium was atomic absorption spectrophotometry. The results are shown in Table 1-Table 3. ...

Embodiment 3

[0051] Embodiment 3: the activation of soil nutrient by composite biological bacterial agent

[0052] The composite biological bacterial agent of embodiment 1 is mixed in the soil by the inoculum amount of 1% (v / w), and control group receives equal amount of sterilized water, and after inoculating, the mouth of the bottle is wrapped with 8 layers of gauze, and soil is measured after 28 days Nutrient change, measurement method: pH is measured by a pH meter with a water-to-soil ratio of 1:1, soil organic matter is measured by potassium dichromate volumetric method, soil ammonium nitrogen is measured by Nessler's reagent colorimetry, and soil available phosphorus is measured by NaHCO 3 Molybdenum antimony anti-colorimetric method for leaching, and ammonium acetate leaching-atomic absorption spectrophotometry for soil available potassium. The results are shown in Table 4.

[0053] Table 4 Effects of compound biological agents on soil physical and chemical properties

[0054] ...

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Abstract

The invention provides a composite microbial agent capable of improving soil fertility, and a preparation method and application thereof. The composite microbial agent comprises, by volume, 80 to 95%of composite bacterial liquid and 5 to 20% of biostimulant, wherein the composite bacterial liquid is composed of Bacillus megaterium fermentation broth and Paenibacillus mucilaginosus fermentation broth; each fermentation broth contains more than 5*10<8> CFU / mL of effective live bacteria; and the composite bacterial liquid contains 1*10<9> CFU / mL of effective live bacteria altogether. The composite microbial agent provided by the invention plays an important role in improving soil fertility, alleviating soil salinization, reducing resource wastage and increasing crop yield.

Description

technical field [0001] The invention belongs to the field of microbial fertilizers and relates to a composite biological bacterial agent for improving soil fertility. Background technique [0002] my country feeds 21% of the world's population with 9% of the world's arable land and 6% of water resources. It is a great achievement and is universally recognized. However, in order to feed 1 / 5 of the world's population, our chemical fertilizer consumption accounts for 1 / 3 of the world's total. . The long-term and large-scale use of chemical fertilizers leads to the destruction of soil structure, the decline of soil organic matter, and the imbalance of soil micro-ecological environment, which in turn leads to the decline of soil fertility, the aggravation of plant diseases and insect pests, soil acidification and salinization. Excessive chemical fertilizers, except that some of them enter the lake through rainwater and cause eutrophication, most of them are fixed in the soil and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/04C05F11/08C12N1/20C12R1/11C12R1/01C05G3/80
Inventor 李光超罗小艳王誉瑶
Owner 黄山中科新佳生物科技有限公司
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