Novel methylobacterium MR1 and applications thereof

A new type of methyl bacteria, applied in the field of environmental microorganisms, can solve the problems of complex components and unclear composition of activated sludge

Active Publication Date: 2018-02-09
YUNNAN WAN KUI BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There are abundant microorganisms in activated sludge, and some microorganisms have formaldehyde metabolism pathways. Therefore, many research results use domesticated activated sludge to prepare bioreactors. The analysis results show that such bioreactors can remove and degrade formaldehyde and pollutants polluted in the air. Other organic matter, but there are many types of microorganisms in activated sludge, the composition is not clear, and may contain some pathogenic bacteria
In addition, due to the complex composition of activated sludge, the organic matter contained in it may cause the reproduction and growth of mold under high temperature and high humidity conditions.

Method used

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  • Novel methylobacterium MR1 and applications thereof
  • Novel methylobacterium MR1 and applications thereof
  • Novel methylobacterium MR1 and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Screening, identification and detection of embodiment 1 bacterial strain

[0029]In order to overcome the defects of the prior art, the present invention at first embeds the activated sludge collected in the river course with alginate and sponge, utilizes the air polluted with high concentration of formaldehyde to domesticate the microorganisms in the activated sludge, and then utilizes the inorganic salt of methylobacteria The culture medium is used to isolate methylobacteria that grow fast and are highly resistant to formaldehyde. The specific screening steps are:

[0030] 1. Encapsulation of activated sludge and domestication of microorganisms

[0031] Collect the sludge from the river and mix it with water, filter it with gauze to remove large particles such as gravel, then mix it with 2% sodium alginate (1:1), soak the sponge and remove the excess liquid mixture, and then put it in 4 %CaCl 2 Solidified embedding in solution. Roll the sponge embedding sludge into...

Embodiment 2

[0036] Example 2 Analysis of the resistance of MR1 to formaldehyde, the effect of removing formaldehyde and the pathway of metabolic conversion of formaldehyde

[0037] 1. Analysis of the resistance of MR1 to formaldehyde

[0038] Add 4 mM, 8 mM, 12 mM and 20 mM formaldehyde to the solid medium with and without yeast extract, and inoculate 1% (v / v) of MR1 bacterial solution into the liquid culture medium containing 5 mM methanol and yeast extract culture medium at 28°C on a shaking table, and when the OD600 reached 0.5, 1 μl, 2 μl, 3 μl, 4 μl, 5 μl, 6 μl, 7 μl, 8 μl, and 9 μl of bacterial liquid were spotted on solid media containing different concentrations of formaldehyde, and warmed at 28°C. After culturing in the box for 48 hours, observe its growth status. The results showed that there was no significant difference in the growth status of strain MR1 in the inorganic medium with and without yeast extract, indicating that the growth of MR1 was not dependent on yeast extrac...

Embodiment 3

[0047] Example 3 Analysis of MR1's tolerance to benzene series and other organic pollutants and the effect of removing benzene series

[0048] 1. Analysis of tolerance of MR1 to benzene series and other organic pollutants

[0049] Add concentrations of 4 mM, 8 mM, 12 mM and 20 mM ethanol, isopropanol, xylene, benzene, and toluene to solid medium containing 5 mM methanol to make plates. Take 1 μl, 2 μl, 3 μl, 4 μl, 5 μl, 6 μl, 7 μl, 8 μl, 9 μl of MR1 bacterial solution with an OD600 of 0.5 and spot it on a solid plate, culture it in a 28°C incubator for 48 hours, and observe its growth status. The results show that the presence of acetaldehyde, ethanol, and isopropanol has no significant effect on the growth of the MR1 strain. Its tolerance to acetaldehyde, ethanol, and isopropanol can reach 20mM, but the growth status on the plate containing isopropanol poor. It can grow on the plate added with xylene, benzene, and toluene, and the resistance to xylene, benzene, and toluene ...

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Abstract

The invention relates to a methylobacterium zatmanni strain MR1. The methylobacterium zatmanni strain MR1 is preserved in the China Center for Type Culture Collection, and is assigned with the accession number of CCTCC M 2017322. The methylobacterium is methylotrophic bacterium obtained by adopting activated sludge embedded by sodium alga acid domesticated by formaldehyde as the material and through separation of an inorganic salt culture medium of the methylobacterium, the methylobacterium can grow in the culture medium taking formaldehyde as the unique carbon source, has the resistance achieving 30mM for formaldehyde on a solid culture medium, grows normally in a liquid culture medium in which 5mM methanol and 2-15 mM formaldehyde are added, and has relatively high removal rate for formaldehyde with high concentration in the culture medium. MR1 also can grow in the inorganic salt culture medium adopting sodium acetate, sodium malate or sodium citrate as the unique carbon source, andthe growth is the best when sodium malate is adopted as the carbon source. When MR1 grows in the culture medium containing sodium acetate, sodium malate or sodium citrate, the added benzene series compounds can be removed, and the removal rate achieves 100%.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and specifically relates to a novel methylobacterium strain with a special formaldehyde metabolism pathway, which can be applied to the removal of formaldehyde and benzene series pollution. Background technique [0002] Formaldehyde (HCHO) is an air pollutant that commonly exists in the indoor environment. Formaldehyde pollution is found in various places after decoration, many factories and pharmaceutical reagent storage warehouses. At the same time, it is also produced as an endogenous metabolite in all In organisms, formaldehyde can be produced by spontaneous dissociation of 5,10-methylenetetrahydrofolate in organisms; in addition, various demethylation reactions can also produce low concentrations of formaldehyde. In the long-term evolution process, almost all organisms have produced formaldehyde detoxification mechanism to prevent the lethal and mutation effects of formaldehyde. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84C12R1/01
CPCB01D53/84B01D2251/95B01D2257/7027B01D2257/704B01D2257/708B01D2258/012B01D2258/06C12N1/205C12R2001/01Y02A50/20
Inventor 陈丽梅李昆志王茹冯永
Owner YUNNAN WAN KUI BIOLOGICAL SCI & TECH CO LTD
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