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Preparation method of BC (bacterial cellulose) with high deformation resistance

A technology of bacterial cellulose, deformability, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve low-cost effects

Inactive Publication Date: 2018-02-09
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, bacterial cellulose is mostly modified by adding carboxymethyl cellulose, microcrystalline cellulose, starch and other polysaccharides to the liquid medium. At present, it has not been seen to add agar to the medium to produce bacterial cellulose under semi-solid conditions. report

Method used

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  • Preparation method of BC (bacterial cellulose) with high deformation resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Medium preparation (g / L): glucose 7.5, yeast powder 10, peptone 10, Na 2 HPO4 10, adjust the pH to 6.0 with glacial acetic acid, weigh 0.05% (w / v) agar into the liquid medium, and sterilize at 121° C. for 20 minutes.

[0029] (2) Strain activation: pick the Gluconacetobacter xylinum obtained from the three-section line on the plate and put it in a 500mL Erlenmeyer flask containing 150mL liquid medium, shake it at 30°C and 180r / min for 24h, and then inoculum with 6% Transfer to another two bottles of fresh culture medium, and cultivate to the logarithmic phase at 30°C and 180r / min shaking.

[0030] (3) Inoculation: Mix the two bottles of seed liquid in (2) evenly, inoculate into liquid medium, semi-solid medium and solid medium with 6% inoculum amount, and place it in a constant temperature incubator at 30°C for static cultivation 5 days.

[0031] (4) Bacterial cellulose extraction and treatment: use the membrane after it is taken out, and rinse with water several ...

Embodiment 2

[0034] (1) Medium preparation (g / L): glucose 7.5, yeast powder 10, peptone 10, Na 2 HPO4 10, adjust the pH to 6.0 with glacial acetic acid, weigh 0.15% (w / v) agar into the liquid medium, and sterilize at 121° C. for 20 minutes.

[0035] (2) Strain activation: pick the Gluconacetobacter xylinum obtained from the three-section line on the plate and put it in a 500mL Erlenmeyer flask containing 150mL liquid medium, shake it at 30°C and 180r / min for 24h, and then inoculum with 6% Transfer to another two bottles of fresh culture medium, and cultivate to the logarithmic phase at 30°C and 180r / min shaking.

[0036] (3) Inoculation: Mix the two bottles of seed liquid in (2) evenly, inoculate into liquid medium, semi-solid medium and solid medium with 6% inoculum amount, and place it in a constant temperature incubator at 30°C for static cultivation 5 days.

[0037] (4) Bacterial cellulose extraction and treatment: use the membrane after it is taken out, and rinse with water several ...

Embodiment 3

[0040] (1) Medium preparation (g / L): glucose 7.5, yeast powder 10, peptone 10, Na 2 HPO4 10, adjust the pH to 6.0 with glacial acetic acid, weigh 0.2% (w / v) agar into the liquid medium, and sterilize at 121° C. for 20 minutes.

[0041](2) Strain activation: pick the Gluconacetobacter xylinum obtained from the three-section line on the plate and put it in a 500mL Erlenmeyer flask containing 150mL liquid medium, shake it at 30°C and 180r / min for 24h, and then inoculum with 6% Transfer to another two bottles of fresh culture medium, and cultivate to the logarithmic phase at 30°C and 180r / min shaking.

[0042] (3) Inoculation: Mix the two bottles of seed liquid in (2) evenly, inoculate into liquid medium, semi-solid medium and solid medium with 6% inoculum amount, and place it in a constant temperature incubator at 30°C for static cultivation 5 days.

[0043] (4) Bacterial cellulose extraction and treatment: use the membrane after it is taken out, and rinse with water several ti...

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Abstract

The invention discloses a preparation method of BC (bacterial cellulose) with high deformation resistance. The method comprises the steps as follows: (1) agar in the ratio being 0.05%, 0.15% and 0.2%(W / V) is added to a fluid medium, sterilization is performed at 121 DEG C for 20 min, and a semi-solid medium is prepared; (2) the semi-solid medium is inoculated with gluconacetobacter xylinus, the semi-solid medium is placed in a constant-temperature incubator at 30 DEG C for culture for 5 days, and a BC membrane is produced; (2) the membrane is taken out and washed with water repeatedly for removal of the medium and impurities on the membrane surface, the membrane is soaked in 0.1 mol / L of a NaOH solution until the membrane is milky white and semi-transparent, then, the membrane is washed with distilled water repeatedly, and the pH value of the membrane is neutral through slight press of pH test paper; (3) the BC is pressed to be dried at 80 DEG C for mechanical performance tests. The Young modulus of the BC membrane is enlarged, the larger the Young modulus, the probability of deformation is lower, the application range of the BC is further broadened and the application value is increased with the preparation method, and a foundation is laid for preparation of multiple multifunctional composite materials with excellent performance.

Description

technical field [0001] The invention relates to a preparation method of bacterial cellulose, more specifically to a method for preparing bacterial cellulose in a semi-solid medium. Background technique [0002] Cellulose (Bacterial cellulose, BC for short) is a kind of pure cellulose produced by microorganisms. The bacteria capable of producing cellulose mainly include Acetobacter, Agrobacterium, Pseudomonas, Achromobacter, Alcaligenes, Aerobacter, Azotobacter, Rhizobium and Sarcina. Bacterial cellulose is chemically identical to cellulose produced by plants or algae. However, as a new type of biomaterial, bacterial cellulose has high crystallinity and chemical purity, high tensile strength and elastic modulus, excellent shape maintenance ability and tear resistance, high biocompatibility and good biological properties. Degradability, controllability of performance during biosynthesis and other characteristics. Due to its unique properties, bacterial cellulose has broad p...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/02
CPCC12P19/04
Inventor 钟成谢永振贾士儒李思琦侯颖
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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