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A kind of cationic polymer and its preparation method and application

A polymer and copolymer technology, applied in the field of cationic polymers and their preparation, can solve the problems of insufficient protonation buffer capacity, difficult drug and gene release, and lack of endosome destruction ability, and achieve good biocompatibility and Biodegradable performance, good transfection effect, easy to popularize effect

Active Publication Date: 2021-03-30
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of these cationic polymer materials lack degradability, which not only makes the release of drugs and genes difficult, but also may lead to toxicity to organisms.
As a cationic polypeptide, polylysine has good biocompatibility, excellent biodegradability and unique advanced conformation, but its side chain cationic groups are mainly primary amine structures, and the protonation buffer capacity is not high enough , leading to relatively low cell entry efficiency, lack of endosome disrupting ability, and transfection activity is an order of magnitude lower than that of branched PEI, which limits its application

Method used

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  • A kind of cationic polymer and its preparation method and application
  • A kind of cationic polymer and its preparation method and application
  • A kind of cationic polymer and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Add 0.2g polylysine (number-average molecular weight: 1500) and 0.01g potassium hydroxide to the reaction flask, add 8 mL of acetonitrile / distilled water (v:v=2:1) ​​mixed solvent and stir to dissolve; then add 2,3-cyclo Oxypropyltrimethylammonium chloride (0.49g, the molar ratio of it to lysine residues, that is, the reaction equivalent ratio is 2:1) in ethanol solution, reacted at room temperature for 12h; after the reaction, the reaction solution was dialyzed After freeze-drying, the cationic polymer was obtained.

Embodiment 2

[0048] 0.45g polyethylene glycol-polylysine copolymer (polyethylene glycol number average molecular weight is 5000, polylysine number average molecular weight is 2560) and 0.02g sodium hydroxide are added reaction flask, add methanol / distilled water ( v:v=3:1) mixed solvent 12mL stirred and dissolved; then added (3-dimethylaminopropyl) acrylamide (0.58g, its molar ratio to lysine residues, that is, the reaction equivalent ratio was 3: 1) was reacted in methanol solution under ice bath for 24 hours; after the reaction was completed, the reaction solution was dialyzed and freeze-dried to obtain a cationic polymer.

Embodiment 3

[0050] Add 0.45g of polyethylene glycol-polylysine copolymer (the number average molecular weight of polyethylene glycol is 5000, and the number average molecular weight of polylysine is 2560) and 0.025g of sodium hydroxide into the reaction flask, add 12mL of tetrahydrofuran and stir to dissolve ; Then add (3-acrylamidopropyl)-trimethylammonium chloride (0.77g, its molar ratio with lysine residues, that is, the reaction equivalent ratio is 3:1) methanol solution, under ice bath React for 2 hours, and then react for 48 hours at room temperature; after the reaction, the reaction solution is dialyzed and freeze-dried to obtain a cationic polymer.

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Abstract

The invention belongs to the technical field of polymer materials, and in particular relates to a cationic polymer and its preparation method and application. The present invention provides a kind of cationic polymer, described cationic polymer contains the structure described in formula I, in formula I, R 1 It is a tertiary amine or quaternary ammonium group with 5 to 40 carbon atoms, R 2 is a hydrogen atom or R 1 ;0≤m<156,0

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a cationic polymer and its preparation method and application. Background technique [0002] In recent decades, polymer nanomaterials have been extensively and intensively studied due to their promising applications in the fields of drug delivery, gene transfection, and anti-infection. However, due to the complex physiological environment in the human body, it is difficult for polymer nanomaterials to cross multiple physiological barriers and cell barriers to reach the target. Since the cell membrane surface of all organisms is negatively charged (T.Yeung, et al., Science, 2008, 319, 210-213), researchers have found that nanoparticles with cations can interact with the negatively charged cell surface through electrostatic Combined with the interaction, and effectively penetrate the cell membrane (A. Verma, et al., Small, 2010, 6, 12-21; Y. Obata, et al., Jo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G69/48C08F287/00C08F283/00C08F220/60C08F220/34A61K47/32A61K47/34A61K48/00A61P31/04A61P31/10
CPCA61K47/32A61K47/34A61K48/00C08F283/00C08F287/00C08G69/48C08F220/60C08F220/34
Inventor 丁明明刘航成澄杨艳雪谭鸿傅强
Owner SICHUAN UNIV
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