Method for measuring vitamin D3 in soybean protein powder

A technology of soybean protein powder and a determination method, which is applied in the field of determination of vitamin D3 in soybean protein powder, can solve the problems of difficulty in accurately controlling the outflow time of vitamin D3, easily destroyed vitamin content, high cost and high cost, and achieves the goal of being beneficial to popularization, popularization and use, and wide application. Market application prospects, accurate and easy-to-operate effects
CN107703239AInactive Publication Date: 2018-02-16GUANGZHOU SAIJIAN BIO TECH
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGZHOU SAIJIAN BIO TECH
Publication Date
2018-02-16
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention relates to the technical field of analysis of functional components in health food, in particular to a method for measuring vitamin D3 in soybean protein powder. The method comprises thesteps of saponifying, extracting, washing, concentrating, purifying and carrying out high performance liquid chromatography, wherein the step of purifying comprises the processes of sequentially carrying out activation, balancing, sample loading, leaching and elution on a silica gel solid phase extraction column by using chemical methods. Impurities are firstly removed by means of the step of leaching, and the vitamin D3 is completely dissolved and eluted by means of the step of elution, so that all the vitamin D3 in a sample to be measured can be obtained only by collecting eluate, and the amount of the vitamin D3 can be accurately controlled. Compared with the prior art, the improved purifying step of the method is very simple, accurate and easy to operate, and the detection cost is greatly lowered, so that the method has wide market application prospect.
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Description

technical field

[0001] The invention relates to the technical field of functional component analysis of health food, in particular to a method for measuring vitamin D3 of soybean protein powder. Background technique

[0002] Vitamin D3 is a sterol derivative, also known as cholecalciferol, which is mainly derived from dietary intake or synthesis in the body under ultraviolet radiation. It can promote the absorption of calcium and phosphorus in the body, and can treat children's rickets and adult calcium deficiency. Play a very good preventive role. Therefore, vitamin D3 is often used in calcium-supplementing health foods.

[0003] With the increasing demand for health food testing, the current detection method for vitamin D3 mainly uses high-performance liquid chromatography, which is mainly based on the fourth method of GB5009. Liquid chromatography, the defects of this detection method are: 1) The steps are cumbersome and the operation time is long, and the technical req...

Claims

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