Method for measuring vitamin D3 in soybean protein powder

Abstract

The invention relates to the technical field of analysis of functional components in health food, in particular to a method for measuring vitamin D3 in soybean protein powder. The method comprises thesteps of saponifying, extracting, washing, concentrating, purifying and carrying out high performance liquid chromatography, wherein the step of purifying comprises the processes of sequentially carrying out activation, balancing, sample loading, leaching and elution on a silica gel solid phase extraction column by using chemical methods. Impurities are firstly removed by means of the step of leaching, and the vitamin D3 is completely dissolved and eluted by means of the step of elution, so that all the vitamin D3 in a sample to be measured can be obtained only by collecting eluate, and the amount of the vitamin D3 can be accurately controlled. Compared with the prior art, the improved purifying step of the method is very simple, accurate and easy to operate, and the detection cost is greatly lowered, so that the method has wide market application prospect.

Description

technical field [0001] The invention relates to the technical field of functional component analysis of health food, in particular to a method for measuring vitamin D3 of soybean protein powder. Background technique [0002] Vitamin D3 is a sterol derivative, also known as cholecalciferol, which is mainly derived from dietary intake or synthesis in the body under ultraviolet radiation. It can promote the absorption of calcium and phosphorus in the body, and can treat children's rickets and adult calcium deficiency. Play a very good preventive role. Therefore, vitamin D3 is often used in calcium-supplementing health foods. [0003] With the increasing demand for health food testing, the current detection method for vitamin D3 mainly uses high-performance liquid chromatography, which is mainly based on the fourth method of GB5009. Liquid chromatography, the defects of this detection method are: 1) The steps are cumbersome and the operation time is long, and the technical req...

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Problems solved by technology

[0003] With the increasing demand for health food testing, the current detection method for vitamin D3 mainly uses high-performance liquid chromatography, which is mainly based on the fourth method of GB5009. Liquid chromatography, the defects of this detection method are: 1) The steps are cumbersome and the operation time is long, and the technical requirements for the operator and the conditions of the laboratory equipment are very high, and the operator needs to enter the normal phase immediately after obtaining the pretreated sample liquid The system separates and collects the liquid within the required retention time period, and blows it with nitrogen immediately. If the total processing time is too long, the vitamin content will be easily destroyed; 2) When collecting the vitamin D3 fraction, the operator must first observe the liquid in the liquid to be tested. The retention time of vitamin D3 on the liquid phase instrument, and then collect its fraction at the outlet of the pipeline, so it is difficult to accurately control the outflow time of vitamin D3 in the pipeline, resulting in the loss of vitamin D3; Very high, need two high-performance liquid chromatographs, normal-phase chromatographic columns, the use cost (electricity) of the instrument, and the daily maintenance of the instrument. The cost is very high, which is not conducive to popularization

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