High-throughput microalgae growth testing device and method

Abstract

The invention provides a high-throughput microalgae growth testing device and method. The device comprises a culture frame body, a light source, a CO2 or air source, a digital gas flowmeter, milliliter culture flasks, a gas multi-valve parallel regulator, and a gas filter; the culture frame body is provided with a plurality of locating structures used for accommodating the milliliter culture flasks. The high-throughput microalgae growth testing device not only keeps the various advantages of small system, high parallelity, wide scope of application and the like, but also makes a breakthrough on the aspects of high throughput, flexibility and stability, the high throughput of 1200 groups of reaction culture flasks at most is realized in one cubic meter, and the time spent on research is greatly saved. The illumination and the air supply for one single reaction culture flask are accurately controlled; the in-situ sampling is realized through an injector, so that the sampling is accurate,the risk of contamination is also reduced, the reaction system is constant in the whole culture process, and the accuracy of the result is improved.

Description

technical field [0001] The invention relates to the technical field of photosynthetic microorganism cultivation and detection, in particular to a high-throughput microalgae growth testing device and method. Background technique [0002] The cultivation and testing of microalgae is an indispensable part of phycology research. In the research of microalgae bioenergy, ecotoxicology, water eutrophication, water body plankton community structure and many other fields, generally by setting a normal control group Compared with the treatment group (such as adding toxic and harmful components such as heavy metals; adding sewage with high N and P content; adding different doses of nutrient salts; adding different algae species; adding different plankton, etc.), compare the growth of the two groups of algal cells, and determine Important evaluation indicators such as algae biomass, oil pigment content, photosynthetic efficiency, and culture medium composition provide data support for t...

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Problems solved by technology

[0004] However, the culture system of Erlenmeyer flasks and well plate dishes has many shortcomings: for example, gas sources such as carbon dioxide cannot be supplied, the cells in the culture system are not uniformly dispersed, and the growth rate is slow; the culture space is easily limited, and for tens of milliliter systems, the unit The number of groups in the space setting is limited, that is, the flux is low; the optical density of each group is easily uneven, and the parallelism is poor; the sampling is cumbersome, easy to be infected with bacteria, and the culture system is not stable enough, etc.

[0006] However, these photobioreactors do not reflect the importance of throughput, generally only a few or more than a dozen groups of reaction units can be set up, and the culture system is relatively large

With the rapid development of microalgae biotechnology, microalgae culture tests are more widely used in ecotoxicology, water eutrophication, microalgae bioenergy and other fields. Low-throughput reactors will greatly increase the time cost of research. , hindering the rapid development of microalgae biotechnology

And the current photoreactor has done a lot of work on parallelism, but it ignores the flexible regulation of a single reactor unit, which is not enough to support more complex and changeable experimental research

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