One-dimensional calcium phosphate nano/micron material as well as preparation method and application thereof
A calcium phosphate and nanotechnology, applied in the field of gene carrier, can solve the problems of difficult control, low transfection efficiency, and expensive phospholipid materials, and achieve the effect of high yield and simple preparation process
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[0077] The invention provides a preparation method of a one-dimensional calcium phosphate nano / micro material, comprising the following steps:
[0078] (1) 3mLNaH 2 PO 4 solution (0.05mol / L) and 3mL of CaCl 2 The solution (0.1mol / L) was added to a mixed solvent composed of 15mL deionized water and 15mL absolute ethanol, and mixed uniformly at room temperature; then the pH value of the mixed solution was adjusted to 7.0±0.5 to obtain a reaction solution A. (2) Pour the reaction solution A into a microwave hydrothermal reaction kettle, heat it with microwave at 110°C for 10 minutes, and take out the reaction kettle when the system naturally cools below 60°C; (3) Centrifuge the product in the reaction kettle at 12000g 10 min, and the separated solid was washed 3 times with absolute ethanol, and finally freeze-dried to obtain a calcium phosphate nano / micro material, and this sample was named sample 1.
[0079] It should be noted that, according to different needs, the pH of sol...
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