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Dendrobium crepidatum tissue culture method

A technology of Dendrobium rosea and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low rooting rate, low survival rate, low germination rate, etc. rooting effect

Inactive Publication Date: 2018-04-20
容县明曦铁皮石斛种植场(微型企业)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But no matter adopting the method of rooting outside the bottle or rooting in the bottle, there are still problems of low germination rate and low rooting rate, and the survival rate of seedlings is very low.

Method used

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  • Dendrobium crepidatum tissue culture method
  • Dendrobium crepidatum tissue culture method
  • Dendrobium crepidatum tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0025] Take mature Dendrobium rosea seeds for disinfection treatment, rinse with tap water and detergent for 3 minutes, then disinfect with 75% alcohol for 30 seconds, then disinfect with 35% hydrogen peroxide for 10 minutes, finally rinse with sterile water three times and sow in the seed medium Cultivate, after turning green, continue to cultivate for 20-30 days, after the seed embryo expands and germinates to form a green protocorm. The culture conditions are 25-26°C, the light is 1600-1800lx, and the light time is 10h / d.

[0026] The seed culture medium that the present invention adopts is to add NAA 0.5-2.0mg / L, 6-BA0.5-3.0mg / L, potato 100000mg / L and The composition of hydrolyzed milk protein 700mg / L. The seed improved MS medium contains the following components: potassium nitrate 1600mg / L, ammonium nitrate 1250mg / L, magnesium sulfate 370mg / L, potassium dihydrogen phosphate 170mg / L, calcium chloride 440mg / L, copper sulfate 0.025mg / L L, cobalt chloride 0.025mg / L, mangane...

experiment example 2

[0031] Transfer the protocorms of Dendrobium rosea to the strong seedling medium for strong seedling cultivation, and carry out the strong seedling cultivation for 80-90 days. The cultivation conditions are: temperature 25-26°C, light intensity 1900-2100lx, light time 12h / d.

[0032] The strong seedling culture medium that the present invention adopts is to add NAA0.5~2.0mg / L, 6-BA0.5~3.0mg / L and banana on the basis of strong seedling improved MS medium (hereinafter referred to as strong seedling improved MS) 200000mg / L composition. Among them, the improved MS medium for strong seedlings contains the following components: potassium nitrate 2000mg / L, ammonium nitrate 1750mg / L, magnesium sulfate 370mg / L, potassium dihydrogen phosphate 170mg / L, calcium chloride 550mg / L, copper sulfate 0.025mg / L, cobalt chloride 0.025mg / L, manganese sulfate 16.9mg / L, zinc sulfate 8.6mg / L, boric acid 6.2mg / L, potassium iodide 0.83mg / L, sodium molybdate 0.25mg / L, Na 2 -EDTA 37.3mg / L, ferrous sulfa...

experiment example 3

[0037] The seedlings obtained in step (2) are transferred to the rooting medium to induce rooting, and grow into rooted seedlings with a well-developed main root system.

[0038] The rooting culture medium that the present invention adopts is to add NAA0.5-2.0mg / L, 6-BA0.5-3.0mg / L and Ai Bidi on the basis of rooting improved MS medium (hereinafter referred to as rooting improved MS) The composition of powder is 0.5-2.5mg / L. Among them, the rooting improved MS medium contains the following components: potassium nitrate 1650mg / L, ammonium nitrate 1350mg / L, magnesium sulfate 270mg / L, potassium dihydrogen phosphate 130mg / L, calcium chloride 450mg / L, copper sulfate 0.025mg / L L, cobalt chloride 0.025mg / L, manganese sulfate 16.9mg / L, zinc sulfate 8.6mg / L, boric acid 6.2mg / L, potassium iodide 0.83mg / L, sodium molybdate 0.25mg / L, Na2-EDTA 37.3mg / L L, ferrous sulfate 27.8mg / L, glycine 2.0mg / L, thiamine hydrochloride 0.2mg / L, pyridoxine hydrochloride 0.65mg / L, niacin 0.5mg / L, inositol 5...

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Abstract

The invention discloses a dendrobium crepidatum tissue culture method. The method comprises the following four steps: culturing and germinating dendrobium crepidatum seeds to obtain protocorm, performing strong seedling culture, performing root induction and hardening seedlings. In the step of culturing and germinating dendrobium crepidatum seeds to obtain protocorm, the used seed medium comprisesthe following components: 1600mg / L of potassium nitrate, 1250mg / L of ammonium nitrate, 370mg / L of magnesium sulfate, 170mg / L of monopotassium phosphate, 440mg / L of calcium chloride, 0.025mg / L of copper sulfate, 0.025mg / L of cobalt chloride, 16.9mg / L of manganese sulfate, 8.6mg / L of zinc sulfate, 6.2mg / L of boric acid, 0.83mg / L of potassium iodide, 0.25mg / L of sodium molybdate, 37.3mg / L of Na2-EDTA, 21.8mg / L of ferrous sulfate, 2.0mg / L of glycine, 0.1mg / L of thiamine hydrochloride, 0.5mg / L of pyridoxine hydrochloride, 0.5mg / L of nicotinic acid, 100mg / L of inositol, 0.5-2.0mg / L of NAA, 0.5-3.0mg / L of 6-BA, 100000mg / L of potatoes, 250mg / L of carbon dust, 30000mg / L of sucrose, 700mg / L of lactoalbumin hydrolysate and 4500mg / L of agar. According to the method, the dendrobium crepidatum seed germination rate is high to 90% or higher, and the reproduction rate is greatly improved.

Description

technical field [0001] The invention relates to a tissue culture method of Dendrobium rosea. Background technique [0002] Dendrobium rose (scientific name: Dendrobium crepidatum Lindl.ex Paxt.): the stem is pendulous, fleshy and thick, green, cylindrical, unbranched, multi-noded, and the length of the internode is 3-4 cm. Leaves subleathery, narrowly lanceolate. The raceme is very short, issued from the upper part of the old stem with fallen leaves, with 1-4 flowers; the flower texture is thick, spreading; the sepals and petals are white, the middle and upper part are lavender, waxy after drying; the petals are broad obovate, long 2.1 cm, 1.2 cm wide, apex subrounded, with 5 veins; lip above middle part lavender, below middle part golden yellow, subrounded or broadly obovate. The flowering period is March-April. Born on tree trunks or rocks in valleys in mountainous forests at an altitude of 1000-1800 meters. Distributed in China, India, Nepal, Sikkim, Bhutan, Myanmar, ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曾少兰涂湘炎吴华球
Owner 容县明曦铁皮石斛种植场(微型企业)