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Citrobacter sp. strain and application thereof

A technology of Citrobacter and citric acid is applied in the field of environmental microorganisms to achieve the effects of high bacterial activity, stable removal effect and strong adaptability

Active Publication Date: 2018-04-20
江苏博恩环境工程成套设备有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no relevant report on the removal of hydrogen sulfide and ammonia by Citrobacter

Method used

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  • Citrobacter sp. strain and application thereof
  • Citrobacter sp. strain and application thereof
  • Citrobacter sp. strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] This embodiment is the screening and identification of deodorizing strain ZT-S5:

[0033] 1. Cultivate

[0034] The medium used in the following experiments was sterilized at 121°C for 20 minutes during the preparation process.

[0035] Luria-Bertani medium (LB): tryptone 10.0g, sodium chloride (NaCl) 10.0g, yeast extract 5.0g, agar powder 15.0g, add distilled water to 1000mL, pH=7.4, sterilize at 121°C for 20min. Its 12-hour growth curve in LB is as follows figure 1 As shown, the strain can grow to the maximum concentration on LB medium after 18 hours.

[0036] Beef extract-peptone medium (NA): 3.0g beef extract, 5.0g sodium chloride (NaCl), 10.0g peptone, 15.0g agar powder, add distilled water to 1000mL, pH=7.5±0.1, sterilize at 121°C for 20min.

[0037] Ammonia enriched medium: 5.0 g sucrose, 2.0 g peptone, potassium dihydrogen phosphate (KH 2 PO 4 ) 2.0g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.5g, sodium chloride (NaCl) 2.0g, zinc sulfate (ZnSO 4 ) 0.05g, ferr...

Embodiment 2

[0063] In this example, strain ZT-S5 is used for deodorization in the whole process.

[0064] The deodorized wastewater sample is the untreated sewage of Nanjing Chengbei Sewage Treatment Plant. Since ammonia and hydrogen sulfide are mainly produced by the decomposition of organic matter by microorganisms under anaerobic conditions, the experimental condition is to add inoculum to the static sewage. Deodorize the activated sludge of the strain, and measure the content of ammonia and hydrogen sulfide in the sewage at different times, and calculate the removal rate.

[0065] The specific process is:

[0066] (1) Add the ZT-S5 bacterial solution cultivated for 12 hours into the sludge, and stir evenly. The control group is the culture medium without adding bacteria and is added to the sludge according to the same inoculum amount.

[0067] (2) Pack the uniformly stirred sludge into large test tubes, fill it to 1 / 5 of the test tube, and fill the test tube with sewage. The CFU coun...

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Abstract

The invention belongs to the field of environmental microbiology, and relates to a Citrobacter sp. strain and application thereof. The Citrobacter sp. is named as deodorizing bacteria ZT-S5, and is collected in the China General Microbiological Culture Collection Center (CGMCC) with the collection number of CGMCC No.15120 on December 25, 2017. The Citrobacter sp. strain provided by the invention has high vitality and high adaptability, and can be widely applied to the biological treatment process of hydrogen sulfide and ammonia in water and gas. A biological deodorizing device made of the strain by means of adsorption can resist the impact of waste gas well, and the application of biological deodorization is realized.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and relates to a Citrobacter strain and an application thereof. Background technique [0002] With the continuous development of the economy, the continuous advancement of the urbanization process, and the continuous improvement of people's living standards, municipal treatment facilities such as sewage treatment plants, sewage lifting stations, garbage transfer stations, and landfills are getting closer and closer to people's living areas. The malodorous exhaust gas produced during the operation of these facilities has become an important factor affecting people's normal life. Malodorous exhaust gas mainly includes sulfur-containing compounds, nitrogen-containing compounds, halogens and their derivatives, hydrocarbons, oxygen-containing organics, organic acids, etc. [0003] The malodorous gas takes the air as the transmission medium and causes harm to human health through the respir...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B01D53/84B01D53/58B01D53/52C12R1/01
CPCB01D53/52B01D53/58B01D53/84C02F3/34C02F2303/02C12N1/205C12R2001/01Y02A50/20
Inventor 蔡泉生傅小强刘玉安
Owner 江苏博恩环境工程成套设备有限公司
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