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A kind of immobilized enzyme oil spill degradation agent

A technology for immobilizing enzymes and degrading agents, applied in the direction of immobilizing enzymes, immobilizing enzymes on or in inorganic carriers, enzymes, etc., can solve problems such as hindering plant photosynthesis under the ocean, water hypoxia, stimulating red tides, etc. Activity and degradation efficiency, increased contact area, and the effect of a wide working environment

Active Publication Date: 2021-08-10
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ecological hazards caused by oil pollution include: oil covers the sea surface, blocking the gas exchange of oxygen and carbon dioxide, which seriously causes water hypoxia; the opacity of oil will prevent sunlight from entering the ocean, and the dissolved oxygen will decrease It hinders the photosynthesis of plants under the ocean, and indirectly stimulates the occurrence of red tides; the polycyclic aromatic hydrocarbons contained in petroleum are highly toxic to organisms, and are finally transmitted to the human body through bioaccumulation and food chain transfer, which is very harmful to humans. carcinogenesis

Method used

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  • A kind of immobilized enzyme oil spill degradation agent
  • A kind of immobilized enzyme oil spill degradation agent

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Comparison scheme
Effect test

Embodiment 1

[0018] An immobilized enzyme oil spill degradation agent, the mass percentage concentration of sorghum straw activated carbon in the immobilized enzyme oil spill degradation agent is 1.5-2.2%, the mass percentage concentration of sodium alginate is 6.8-7.9%, and the concentration of the compound enzyme is 6×10 6 -8×10 6 cell / g, the salinity of the immobilized enzyme oil spill degradation agent is 5-55‰, the temperature is 10-30°C, the pH is 6.5-9.0, and the degradation rate of petroleum hydrocarbons can reach 70-90%. Compared with free enzyme solution, its degradation rate is increased by 35%-50%, and it has strong environmental tolerance and mechanical properties. Sorghum straw activated carbon greatly improves the mass transfer of immobilized enzyme oil spill degradation agent. The contact area between the enzyme liquid and petroleum is increased, which greatly improves the activity and degradation efficiency of microorganisms.

Embodiment 2

[0020] The preparation method of immobilized enzyme oil spill degradation agent comprises the following steps:

[0021] 1) Enzyme liquid preparation: Suction filter the chlorella liquid after 7 days of cultivation and in the logarithmic growth phase, use a filter membrane of 0.55 μm, remove the filter membrane containing chlorella, freeze it with liquid nitrogen, and then add 2.8 times of PBS buffer solution, the pH of PBS buffer solution is 6.8, and the concentration is 0.35mol / L; grind at 0°C, pour the grinding solution into a centrifuge tube and centrifuge at 0°C, 8500r / min for 30 minutes, take out the upper The clear liquid is the enzyme liquid, which is stored at 0°C; the chlorella in the logarithmic growth phase is filtered, ground, and centrifuged to obtain the enzyme liquid. The preparation process of the enzyme liquid is simple and easy, and extraction at low temperature will not destroy Enzyme activity;

[0022] Phosphoric acid activation: Weigh the dried sorghum st...

Embodiment 3

[0027] An immobilized enzyme oil spill degradation agent, the mass percentage concentration of sorghum straw activated carbon in the immobilized enzyme oil spill degradation agent is 1.5-2.2%, the mass percentage concentration of sodium alginate is 6.8-7.9%, and the concentration of the compound enzyme is 6×10 6 -8×10 6cell / g, the salinity of the immobilized enzyme oil spill degradation agent is 5-55‰, the temperature is 10-30°C, the pH is 6.5-9.0, and the degradation rate of petroleum hydrocarbons can reach 70-90%. Compared with free enzyme solution, its degradation rate is increased by 35%-50%, and it has strong environmental tolerance and mechanical properties. Sorghum straw activated carbon greatly improves the mass transfer of immobilized enzyme oil spill degradation agent. The contact area between the enzyme liquid and petroleum is increased, which greatly improves the activity and degradation efficiency of microorganisms.

[0028] The preparation method of the immobil...

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Abstract

The invention discloses an immobilized enzyme oil spill degradation agent. The mass percentage concentration of sorghum straw activated carbon in the immobilized enzyme oil spill degradation agent is 1.5-2.2%, and the mass percentage concentration of sodium alginate is 6.8-7.9%. The compound enzyme concentration is 6×10 6 -8×10 6 cell / g, the salinity of the immobilized enzyme oil spill degradation agent is 5-55‰, the temperature is 10-30°C, the pH is 6.5-9.0, and the degradation rate of petroleum hydrocarbons can reach 70-90%. The preparation method includes: enzyme solution preparation, phosphoric acid activation, steam activation, adsorption and cross-linking. The beneficial effects are: the immobilized enzyme oil spill degradation agent has strong environmental tolerance and mechanical properties, the sorghum straw activated carbon greatly improves the mass transfer of the immobilized enzyme oil spill degradation agent, and increases the contact area between the enzyme liquid and oil Large, greatly improving the activity and degradation efficiency of microorganisms.

Description

technical field [0001] The invention relates to the field of oil degradation agents, in particular to an immobilized enzyme oil spill degradation agent. [0002] technical background [0003] There are roughly two types of oil pollution: sudden input and chronic long-term input. Sudden inputs include oil tanker accidents and spills from offshore oil production. Chronic long-term input such as oily sewage discharge from ports and ships, natural seabed seepage, etc. Offshore oil pollution seems far away, but it is actually closely related to our lives. The ecological hazards caused by oil pollution include: oil covers the sea surface, blocking the gas exchange of oxygen and carbon dioxide, which seriously causes water hypoxia; the opacity of oil will prevent sunlight from entering the ocean, and the dissolved oxygen will decrease It hinders the photosynthesis of plants under the ocean, and indirectly stimulates the occurrence of red tides; the polycyclic aromatic hydrocarbons...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/18C12N11/14C12N11/10C12N11/089B09C1/10C02F3/34C02F101/32
CPCB09C1/10C02F3/342C02F3/344C02F2101/32C12N11/08C12N11/10C12N11/14C12N11/18
Inventor 陈庆国鲍博李晶晶刘梅穆军
Owner ZHEJIANG OCEAN UNIV
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