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Scotogramma trifolii rottemberg attractant composition and preparation method of nano preparation thereof

A technology of Spodoptera frugiperda and nano-preparation, which can be applied in the fields of botanical equipment and methods, insect attractants, chemicals used for biological control, etc. Insufficient board viscosity and other problems, to achieve the effect of good environmental compatibility, reducing the dosage, and enhancing immunity

Active Publication Date: 2018-04-24
河北军星生物化工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the strong volatility of pheromones, the current pheromone lure carrier has the defect of short duration, and it is necessary to screen and optimize the lure carrier to provide its long-term slow-release performance
When trapping with sex pheromones, the attracted adults often break free due to insufficient sticky board viscosity or insufficient surfactant in the barrel trap

Method used

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  • Scotogramma trifolii rottemberg attractant composition and preparation method of nano preparation thereof
  • Scotogramma trifolii rottemberg attractant composition and preparation method of nano preparation thereof
  • Scotogramma trifolii rottemberg attractant composition and preparation method of nano preparation thereof

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Experimental program
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Effect test

Embodiment 1

[0023] The preparation of embodiment 1 polymer nanofiber

[0024] Dissolve the drug in a specific solvent (tetrahydrofuran: chloroform = 3:1) at a mass ratio of 1:5 to the amphiphilic copolymer. After ultrasonically dissolving completely, stir with a magnetic stirrer at a speed of 500 r / min to homogenize After stirring overnight, use a 5mL syringe pump to add the polymer solution to the electrospinning device at a rate of 0.05mL / min to prepare a 100-450nm non-woven fabric with a drug loading of 10-30% and a wrapping rate of more than 60%. Shaped polymer nanofibers, that is, trapping and killing compositions and nano preparations of Spodoptera moth.

Embodiment 2

[0025] The preparation of embodiment 2 polymer nanofibers

[0026] Dissolve the drug in a specific solvent (tetrahydrofuran: chloroform = 5:1) at a mass ratio of 1:10 to the amphiphilic copolymer. After ultrasonically dissolving completely, stir with a magnetic stirrer at a speed of 500r / min to homogenize After stirring overnight, use a 5mL syringe pump to add the polymer solution to the electrospinning device at a rate of 0.03mL / min to prepare an 80-300nm non-woven fabric with a drug loading of 10-30% and a wrapping rate of more than 75%. Shaped polymer nanofibers, that is, trapping and killing compositions and nano preparations of Spodoptera moth.

Embodiment 3

[0027] Embodiment 3 Antenna potential test

[0028] Place the male Spodoptera worms in a refrigerator at 4°C for 5 minutes, carefully remove the complete antennae from the antennal fossa with precision forceps, and cut off the base of the antennae that the forceps have caught with a sharp blade under a dissecting microscope. In order to ensure the smooth flow of the current path during the test, the antennae were connected to the electrode with a glass pipette electrode with a small hole in the tip and a diameter of 1.5mm filled with 0.1M potassium chloride solution. During EAG measurement, the CS-55 stimulating airflow controller outputs continuous airflow and compensating airflow through the Y-shaped tube, overwash the gas cylinder, and then flow through the flowmeter and then pass through the steel pipe to reach the antennae, and the stimulating airflow passes through a certain volume of Spodoptera moth Pasteur tubes of polymeric nanofiber microspheres of the decoy composit...

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Abstract

The invention discloses scotogramma trifolii rottemberg attractant composition and a preparation method of a nano preparation thereof in the technical field of preparation of agricultural chemicals. According to the preparation method, an amphiphilic copolymer is adopted and subjected to self-assembly in an aqueous solution, nano-sized micelle with an obvious core-shell structure is formed, and anano-pesticide is prepared from the nano-sized micelle with an electrospinning technology. The preparation method is simple and convenient, the raw materials are easily available, the amphiphilic copolymer has quite good environmental compatibility, is biodegradable and is free of residual risk, and furthermore, a degradation product can be absorbed and used by crops; the prepared nano-pesticide is smaller and uniform in grain size and good in permeability and has certain slow-release effect, so that lasting period of scotogramma trifolii rottemberg sex pheromone and pyrethriods pesticides isprolonged, and the use dosage of the pesticide is reduced.

Description

technical field [0001] The invention belongs to the technical field of preparation of agricultural chemicals, and in particular relates to a composition for trapping and killing Spodoptera spp. and a preparation method of nano preparations thereof. Background technique [0002] Spodoptera moth (Scotogramma trifolii Rottemberg), belonging to the order Lepidoptera, family Noctuidae, is an agricultural pest that has caused local outbreaks in farming and pastoral areas in northern my country, and its damage has gradually increased in recent years. The larvae have the characteristics of concealment, explosiveness, and migration hazards. The host crops include cotton, wheat, corn, etc. There are more than 40 kinds of 8 families, including more than 20 kinds of crops and more than 20 kinds of weeds. In Xinjiang, Tibet, Qinghai, Hebei and other provinces and autonomous regions are distributed. Larvae mostly feed on the back of leaves. Young larvae often feed on the mesophyll on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/02A01N31/02A01N53/08A01N25/10A01M1/10A01P7/04A01P19/00
CPCA01M1/106A01N25/10A01N31/02A01N37/02A01N53/00
Inventor 梅向东翁爱珍王安佳王留洋折冬梅宁君
Owner 河北军星生物化工有限公司
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