Antarctic krill peptide preparation method

An Antarctic krill and oligopeptide technology, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of restricting the processing and utilization of Antarctic krill, high fluorine content and high fluorine content in Antarctic krill, and achieves High practical value, low fluorine content and small molecular weight

Inactive Publication Date: 2018-05-01
DALIAN SHENLAN PEPTIDE TECH R & D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Excessive fluorine levels in Antarctic krill
Similarly, Antarctic krill peptide also has the problem of high fluorine content, which limits the further processing and utilization of Antarctic krill. Therefore, it is extremely important to develop low-fluorine hydrolysis technology for Antarctic krill

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Antarctic krill is used as raw material, and the homogenizer is used for chopping and homogenizing.

[0020] (2) Mix Antarctic krill pulp and pure water evenly according to the mass ratio of 1:3, adjust the pH value to 9.0 with sodium hydroxide solution, add 0.5% (total protein) alkaline protease, and stir at 50°C Enzyme hydrolysis for 1h. Heat up to 95°C for 20min to inactivate the enzyme.

[0021] (3) Centrifuge the enzymatic hydrolysis solution after cooling to room temperature, and the centrifuged enzymatic hydrolysis solution is divided into free oil layer + emulsion layer 1, water layer, emulsion layer 2, and residue;

[0022] (4) Collect the aqueous layer solution, adjust the substrate concentration to 5%, adjust the pH to 9.0 with sodium hydroxide solution, add 1.0% (total protein) complex protease, and stir at 50°C for 4 hours. Heat up to 95°C for 20min to inactivate the enzyme.

[0023] (5) Defluorinate the enzymatic hydrolysis solution. Add 0.2% calci...

Embodiment 2

[0027] (1) Antarctic krill is used as raw material, and the homogenizer is used for chopping and homogenizing.

[0028] (2) Mix Antarctic krill pulp and pure water evenly according to the mass ratio of 1:3, adjust the pH value to 9.0 with sodium hydroxide solution, add 0.5% (total protein) alkaline protease, and stir at 50°C Enzymolysis 2h. Heat up to 95°C for 20min to inactivate the enzyme.

[0029] (3) Centrifuge the enzymatic hydrolysis solution after cooling to room temperature, and the centrifuged enzymatic hydrolysis solution is divided into free oil layer + emulsion layer 1, water layer, emulsion layer 2, and residue;

[0030] (4) Collect the aqueous layer solution, adjust the substrate concentration to 10%, adjust the pH to 9.0 with sodium hydroxide solution, add 1.0% (total protein) complex protease, and stir at 50°C for 5 hours. Heat up to 95°C for 20min to inactivate the enzyme.

[0031] (5) Defluorinate the enzymatic hydrolysis solution. Add 0.3% calcium oxide ...

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PUM

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Abstract

The invention provides an Antarctic krill peptide preparation method. Antarctic krills as raw materials undergo homogenization, enzymolysis and centrifuging operation, so that layered enzymatic hydrolysate is obtained, wherein a free oil layer and an emulsion layer can be used for the extraction of Antarctic krill oil. A solution of a water layer is collected, compound protease is added for secondary enzymolysis, enzymatic hydrolysate is added with calcium oxide, and defluorination and centrifuging are carried out. After centrifuging, clear liquid passes through membrane separation equipment to intercept macromolecular substances, filtrate undergoes vacuum concentration, and spray-drying is carried out, so that Antarctic krill oligopeptide powder with low fluorin content and small molecular weight is obtained. The process is easy to operate, the fluorin content of the obtained Antarctic krill peptide is low, the destruction of nutritional ingredients is little, moreover, the molecularweight is low, the absorptivity of a human body is high, and a foundation is laid for the development of functional low-fluorin Antarctic krill oligopeptide.

Description

technical field [0001] The invention relates to the deep processing technology of Antarctic krill. Background technique [0002] Antarctic krill is one of the largest and most successful single species of biological resources on earth. According to statistics, the biomass of Antarctic krill is about 125 to 725 million tons, and the annual catchable amount is more than 13 million tons, which is an important potential fishery resource. Since 2009, my country has started probing Antarctic krill, and the catch in 2013 has exceeded 20,000 tons. The development of Antarctic krill resources has become a national strategy. [0003] Antarctic krill peptides are rich in various amino acids, active peptides, organic acids, etc., especially two kinds of lysine and leucine that are in great demand for the human body, and are easy to absorb, and can be used as a good amino acid nutrition for the human body Supplement; also contains a large amount of highly active angiotensin-converting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/14
CPCC12P21/06C07K1/145C07K1/34
Inventor 包卫洋张延胜王祖哲杨大苹
Owner DALIAN SHENLAN PEPTIDE TECH R & D CO LTD
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