Preparation method of easily-preserved marine small trash enzymolysis full-fish cream rich in small peptides
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A small miscellaneous fish, enzymatic hydrolysis technology, applied in the field of protein enzymatic hydrolysis, can solve the problems of reduced activity, easy deterioration, difficulty in continuous and automatic enzymatic reactions, etc., and achieve the effect of reducing enzymatic hydrolysis time
Active Publication Date: 2018-05-04
玉环市五丰蒸干脱脂鱼粉厂
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[0005] 2) Enzymolysis efficiency is low
Conventional water-bath enzymolysis needs 55-65°C and more than 7 hours, which is time-consuming and labor-intensive, and is easy to deteriorate; due to the defects of free enzymes, the enzymatic hydrolysis reaction is not conducive to the production and preparation of polypeptides, for example: free enzymes in acid, alkali, heat Variability in media such as organic solvents and organic solvents, the activity is reduced or lost; the enzyme remains in the solution after the reaction, making it difficult to continue and automate the enzyme reaction
[0006] 3) How to store, the storage conditions of the proteolysis solution are harsh. In many areas,
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Embodiment 1
[0054] A preparation method for enzymatically hydrolyzed whole fish paste of small marine miscellaneous fish rich in small peptides, which is easy to preserve, comprising the following steps:
[0055] 1) Granulation, using fresh seafood small miscellaneous fish as raw material, freeze-drying to a water content of 50wt%-70wt%, grading and pulverizing to protein particles with a maximum size of less than 5mm, and storing in dry bulk and freezing;
[0056] 2) Micronization, add pure water at 0-3°C to the protein granules according to the mass ratio of protein granules to water 1:0.2, shear at ultra-high speed to a protein granule slurry above 150 mesh, add pure water to the mass ratio of raw fish and water 1:0.2~10.
[0057] More preferably, the mass ratio of feed to water is 1:0.5-5.
[0058] Specifically, the rotation diameter of the shearing blade is 20-40 mm, the motor speed is above 30,000 revolutions, a conical gathering cover is arranged around the blade, and the inner wa...
Embodiment 2
[0083] Such as figure 2 , 3 As shown, in step 4), the preform is a film 5.3, and a plurality of isolation ribs 5.4 are arranged at intervals on the upper surface of the film 5.3, and the isolation ribs 5.4 are sinusoidally spaced at the same period. The upper and lower surfaces of the film 5.3 are evenly bonded with silicon nuclease particles, and the concentration of the immobilized enzyme is determined by the biquinoline formic acid method, reaching 2-3 μg / cm 2 , Practice has proved that the immobilized enzyme has good activity. The film 5.3 is wound around the outer peripheral surface of the shaft 5.1 to form a pre-rolled body 5 with a certain diameter, and the pre-rolled body 5 is fixed in the cylindrical immobilized enzyme reactor 1 . Since the preset winding body 5 has a more compact interval, a sinusoidal curved path is provided between the isolation rib 5.4 and the upper and lower films, and the curved path extends the chance of the enzyme=protease contacting the pr...
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Abstract
The invention discloses a preparation method of easily-preserved marine small trash enzymolysis full-fish cream rich in small peptides. The preparation method comprises the following steps: (1) granulating, taking fresh marine small trash full fish as raw material fish, and carrying out staging smashing until the fish is of protein grains with the maximum sizes of below 5 mm; (2) carrying out micronization, shearing at superhigh speed until the protein particle slurry is 150 meshes or more; (3) carrying out miniaturization, heating until the miniaturization temperature is 55 to 75 DEG C, enabling an ultrasonic probe to extend into an accommodating groove to act on miniaturized ultrasonic waves to prepare miniaturized protein activated slurry; (4) carrying out enzymolysis, heating around microwave until the enzymolysis reaction temperature is 40 to 65 DEG C, putting protease with the raw material fish weight of 0.01% to 2% into the protein particle slurry, carrying out microwave and ultrasonic enzymatic hydrolysis reaction, circularly and alternately applying 10 to 20 minute per time, and carrying out an enzymatic hydrolysis reaction for 20 to 60 min to obtain the enzymolysis protein slurry; carrying out inactivation fermentation, carrying out vacuum concentration until the water content is 40wt% to 60wt%, namely obtaining the easily-preserved marine small trash enzymolysis full-fish cream rich in small peptides. According to the preparation method, the full-fish cream rich can replace a feed protein source as an aquaculture economic feed.
Description
technical field [0001] The invention relates to the technical field of protein enzymatic hydrolysis, in particular to a method for preparing enzymatically hydrolyzed whole surimi of small marine miscellaneous fish that is easy to preserve and rich in small peptides. Background technique [0002] An important and costly raw material in fish feed is protein. A variety of marine fish is the main target of proteolysis. After marine proteolysis, one of the hydrolyzed products is small peptides. Small peptides should be the general term for small molecular peptides, including molecular small peptides with 2 to 3 amino acid residues, and oligopeptides with less than 10 amino acid residues. The left and right small molecule and small peptide products can be classified as small peptide products. Small peptides have good functional properties, not only can be quickly absorbed by organisms in a complete form, directly participate in the synthesis of tissue proteins, but also have phy...
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