Preparation method of easily-preserved marine small trash enzymolysis full-fish cream rich in small peptides

Abstract

The invention discloses a preparation method of easily-preserved marine small trash enzymolysis full-fish cream rich in small peptides. The preparation method comprises the following steps: (1) granulating, taking fresh marine small trash full fish as raw material fish, and carrying out staging smashing until the fish is of protein grains with the maximum sizes of below 5 mm; (2) carrying out micronization, shearing at superhigh speed until the protein particle slurry is 150 meshes or more; (3) carrying out miniaturization, heating until the miniaturization temperature is 55 to 75 DEG C, enabling an ultrasonic probe to extend into an accommodating groove to act on miniaturized ultrasonic waves to prepare miniaturized protein activated slurry; (4) carrying out enzymolysis, heating around microwave until the enzymolysis reaction temperature is 40 to 65 DEG C, putting protease with the raw material fish weight of 0.01% to 2% into the protein particle slurry, carrying out microwave and ultrasonic enzymatic hydrolysis reaction, circularly and alternately applying 10 to 20 minute per time, and carrying out an enzymatic hydrolysis reaction for 20 to 60 min to obtain the enzymolysis protein slurry; carrying out inactivation fermentation, carrying out vacuum concentration until the water content is 40wt% to 60wt%, namely obtaining the easily-preserved marine small trash enzymolysis full-fish cream rich in small peptides. According to the preparation method, the full-fish cream rich can replace a feed protein source as an aquaculture economic feed.

Description

technical field [0001] The invention relates to the technical field of protein enzymatic hydrolysis, in particular to a method for preparing enzymatically hydrolyzed whole surimi of small marine miscellaneous fish that is easy to preserve and rich in small peptides. Background technique [0002] An important and costly raw material in fish feed is protein. A variety of marine fish is the main target of proteolysis. After marine proteolysis, one of the hydrolyzed products is small peptides. Small peptides should be the general term for small molecular peptides, including molecular small peptides with 2 to 3 amino acid residues, and oligopeptides with less than 10 amino acid residues. The left and right small molecule and small peptide products can be classified as small peptide products. Small peptides have good functional properties, not only can be quickly absorbed by organisms in a complete form, directly participate in the synthesis of tissue proteins, but also have phy...

AI Technical Summary

Problems solved by technology

[0005] 2) Enzymolysis efficiency is low

Conventional water-bath enzymolysis needs 55-65°C and more than 7 hours, which is time-consuming and labor-intensive, and is easy to deteriorate; due to the defects of free enzymes, the enzymatic hydrolysis reaction is not conducive to the production and preparation of polypeptides, for example: free enzymes in acid, alkali, heat Variability in media such as organic solvents and organic solvents, the activity is reduced or lost; the enzyme remains in the solution after the reaction, making it difficult to continue and automate the enzyme reaction

[0006] 3) How to store, the storage conditions of the proteolysis solution are harsh. In many areas, due to the unqualified storage technology of the enzymatic solution, the nutrient loss is serious during the storage process of the enzymatic solution, resulting in poor quality of the proteolysis solution and serious waste of raw materials

At present, the research on the storage technology of proteolysis solution mainly focuses on the research of additives in the enzymatic solution. By adding additives, its storage effect can be improved and secondary fermentation can be prevented. However, because the additives are greatly affected by the environment and raw materials, the research results are inconsistent There are differences and it is difficult to achieve large-scale application

Images