Drug-resistance bacteria resisting compound nano preparation and preparation method thereof
A nano-preparation and drug-resistant bacteria technology, which is applied in the direction of antibacterial drugs, pharmaceutical formulas, and medical preparations with non-active ingredients, can solve the problems of antibiotic aggravation, achieve low cost, high activity, and less drug resistance.
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Embodiment 1
[0023] The composite nano-preparation of anti-drug-resistant bacteria of the present invention is prepared by the following method:
[0024] Step 1, dissolve egg yolk lecithin, cholesterol, and dihexadecyl phosphate in 10 mL of chloroform at a ratio of 5:3:1, then add a certain amount of erythromycin, fully dissolve, and rotate to evaporate to obtain a lipid film , hydrated and incubated for 1 hour, vortexed once every 10 minutes, and the hydrated suspension was sonicated in a water bath for 15 minutes, ultrafiltered, centrifuged, and concentrated to obtain liposomes;
[0025] Step 2, add appropriate amount of water and antimicrobial peptide KIWVIRWR to the liposome prepared in step 1, and assemble at 4° C. for 48 hours.
[0026] The initial concentration of antimicrobial peptide was 32 μg / mL, and the initial concentration of erythromycin was 32 μg / mL. Methicillin-resistant Staphylococcus aureus (MRSA) was cultured in MH broth containing oxacillin and sodium chloride respectiv...
Embodiment 2
[0028] The composite nano-preparation of anti-drug-resistant bacteria of the present invention is prepared by the following method:
[0029] Step 1: Dissolve dilinoleoylphosphatidylcholine, cholesterol, 1,2-dioleoyl-sn-glycerol-3-phosphoryl-rac-(1-glycerol) in 10mL at a ratio of 4:1:1 In the mixed solvent of chloroform / methanol, add a certain amount of doxycycline, fully dissolve and then rotary evaporate to obtain a lipid film, hydrate and incubate for 1 hour, vortex once every 10 minutes, and hydrate the suspension in a water bath Ultrasound for 15 minutes, ultrafiltration, centrifugation, and concentration to obtain liposomes;
[0030] Step 2, add appropriate amount of water and antimicrobial peptide RIWVIWRR to the liposome prepared in step 1, and assemble at 4°C for 24 hours.
[0031] Meropenem, doxycycline, and ciprofloxacin were selected to conduct in vitro induction experiments on Acinetobacter baumannii. Three treatment concentrations (selected from 1 / 4, 1 / 2, 1, 2 o...
Embodiment 3
[0034] The composite nano-preparation of anti-drug-resistant bacteria of the present invention is prepared by the following method:
[0035]Step 1, dissolve polyethylene glycol-modified dipalmitoylphosphatidylcholine, cholesterol, and phosphatidylglycerol in a 7:2:1 ratio in 10 mL of chloroform / methanol mixed solvent, and then add a certain amount of thiamphenicol, After fully dissolved, the lipid film was prepared by rotary evaporation, hydrated and incubated for 1 hour, vortexed once every 10 minutes, the hydrated suspension was ultrasonicated in a water bath for 15 minutes, ultrafiltered, centrifuged, and concentrated to obtain liposomes;
[0036] Step 2, add appropriate amount of water and antimicrobial peptide RIWVIRWR to the liposome prepared in step 1, and assemble at 4° C. for 24 hours.
[0037] The initial antimicrobial peptide concentration was 16 μg / mL, and the initial thiamphenicol concentration was 8 μg / mL. Methicillin-resistant Staphylococcus epidermidis (MRSE) w...
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