Cryopreserved protection liquid of human umbilical cord watton's gum tissues, and preparation and application thereof

A Wharton's jelly tissue and protection solution technology, applied in the field of human umbilical cord Wharton's jelly tissue cryopreservation protection solution, can solve the problems of chemical damage to cells and limited improvement of cryopreservation effect, reduce cell activity loss, and facilitate Handling and storage, reducing the effect of physical damage

Inactive Publication Date: 2018-06-22
北京臻溪谷医学研究中心(有限合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, vitrification requires the use of cytotoxic dimethyl sulfoxide, which is likely to cause chemical damage to the cells; and simply increasing or

Method used

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  • Cryopreserved protection liquid of human umbilical cord watton's gum tissues, and preparation and application thereof
  • Cryopreserved protection liquid of human umbilical cord watton's gum tissues, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A cryopreservation solution for human umbilical cord Wharton's jelly tissue, comprising the following components in parts by weight:

[0047] 15 parts of osmotic protectant, 10 parts of non-permeable protectant, 8 parts of protein composition and 1 part of hydrophilic colloid;

[0048] The penetration protectant includes 10 parts of dimethyl sulfoxide and 5 parts of glycerol;

[0049] The non-penetrating protective agent is polyethylene glycol;

[0050] The protein composition includes 5 parts of bovine serum albumin and 3 parts of casein;

[0051] The hydrophilic colloid is gelatin.

Embodiment 2

[0053] A cryopreservation solution for human umbilical cord Wharton's jelly tissue, comprising the following components in parts by weight:

[0054] 20 parts of osmotic protectant, 15 parts of non-permeable protectant, 4 parts of protein composition and 0.8 part of hydrophilic colloid;

[0055] The osmoprotectant includes 12 parts of proline and 8 parts of mannitol;

[0056] The impermeable protective agent includes 10 parts of polysucrose and 5 parts of polyvinylpyrrolidone;

[0057] The protein composition includes 3 parts of casein and 1 part of γ-globulin;

[0058] The hydrophilic colloid includes 0.5 part of gelatin and 0.3 part of tragacanth gum.

Embodiment 3

[0060] A cryopreservation solution for human umbilical cord Wharton's jelly tissue, comprising the following components in parts by weight:

[0061] 15 parts of osmotic protectant, 15 parts of non-permeable protectant, 4 parts of protein composition and 0.8 part of hydrophilic colloid;

[0062] The osmoprotectant includes 3 parts of dimethyl sulfoxide, 3 parts of proline and 9 parts of inositol;

[0063] The impermeable protective agent includes 5 parts of trehalose and 10 parts of hydroxyethyl starch;

[0064] The protein composition includes 1 part of whey protein, 1 part of antifreeze protein and 2 parts of plant-derived recombinant human serum albumin;

[0065] The hydrophilic colloid includes 0.4 part of tamarind gum and 0.4 part of peach gum.

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Abstract

The invention provides cryopreserved protection liquid of human umbilical cord watton's gum tissues, and preparation and application thereof. The electrolyte concentration inside and outside cells arerespectively balanced through a permeating protection agent and a non-permeating protection agent; meanwhile, the free water content is reduced; the formation of ice crystals is reduced; on one hand,the cell damage caused by cell excessive dehydration due to external high-permeation environment can be avoided; on the other hand, the physical damage of the ice crystals on the cells can be reduced; a protein composition is added, so that the required nutrients can be provided for the cells; the cell survival rate is improved; lyophilic colloids can form thixotrope in the water, so that the distribution of all ingredients in the permeating protection agent is uniform; the cells can be uniformly dispersed; precipitates cannot easily occur; in addition, the taking, the use and the storage areeasy. Through the combined use of the ingredients, the activity and the stress resistance of the watton's gum tissues can be effectively improved; the survival rate of the cells during cryopreservation and recovery can be improved; the cell activity loss due to cryopreservation is reduced.

Description

technical field [0001] The invention belongs to the technical field of human tissue cryopreservation, in particular to a human umbilical cord Wharton jelly tissue cryopreservation protection solution and its preparation and application. Background technique [0002] The umbilical cord is a tubular structure that connects the fetus and the placenta in mammals, and is used to provide nutrients to the fetus and transport metabolites. Umbilical cord blood is rich in mesenchymal stem cells. Mesenchymal stem cells are important members of the stem cell family, primitive cells with self-replication and multi-directional differentiation potentials, and primitive cells that form various tissues and organs of the human body. Under certain conditions, it can differentiate into a variety of functional cells or tissues and organs, which are called "universal cells" in the medical field, and are suitable as seed cells for the repair of tissue and organ damage caused by aging and disease. ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 曹毓琳刘俊江时兆田白志惠
Owner 北京臻溪谷医学研究中心(有限合伙)
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