Preparation method of drosophila melanogaster attractant

A trap and fruit fly technology, applied in the field of pesticides, can solve the problems of secondary pollution of the human body and the environment, poor biodegradability, and short effective time, and achieve the effects of protecting the environment, enhancing activity, and improving extraction efficiency

Inactive Publication Date: 2018-06-22
高产明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The technical problem to be solved by the present invention: for the existing fruit fly traps, they are basically chemical compounds with short effective time, poor biodegradability, e

Method used

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  • Preparation method of drosophila melanogaster attractant

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preparation example Construction

[0029] A preparation method of fruit fly attractant, comprising the steps of:

[0030] (1) Take Baker's yeast and add yeast medium at a mass ratio of 1:5, and cultivate at 28-30°C for 18-24 hours. Take the culture and add ethyl acetoacetate and ethyl chloroacetate at a mass ratio of 10:5:2. React at 25~28°C for 10~15h, centrifuge, take the filter residue, add ethyl acetate to the filter residue at a mass ratio of 1:8 and mix, filter through a diatomite adsorption layer, take the filtrate and add sodium chloride at a mass ratio of 1:10, and use acetic acid Ethyl ester extraction, the extract was washed with a 26% sodium chloride solution by mass fraction, dried, and rotary evaporated to obtain a matrix;

[0031](2) Take the base material and add dihydropyran and lithium aluminum hydride in a mass ratio of 1:4:1 to mix, and react at 23~25°C for 1~2h to obtain reactant A. Add p-toluenesulfonyl chloride and mix at a mass ratio of 3:1 to obtain a mixture, take magnesium powder and...

Embodiment 1

[0034] Baker's yeast: purchased from Sigam Company.

[0035] Drosophila larvae reared artificially: the feeding conditions are: temperature 28°C, relative humidity 40%, photoperiod L / D=16h / 8h.

[0036] Soil collection: Take the soil under the bayberry fruit tree, remove the topsoil, select the soil within 20cm below the surface, pass through an 80-mesh sieve to remove stones and debris, mix well, and store in a sealed container at 4°C.

[0037] Separation and purification medium: in parts by mass, take 180 parts of potatoes, 20 parts of glucose, 20 parts of agar, 1000 parts of distilled water, and sterilize at 121°C for 15 minutes.

[0038] Yeast culture medium: in parts by mass, take 1 part of NaH 2 PO 4 , 0.8 parts (NH 4 ) 2 SO 4 , 0.2 parts MgSO 4 ·7H 2 O, 0.3 part CaCl 2 2H 2 O, mix 1 part of yeast extract, 15 parts of glucose, 1 part of peptone, and 500 parts of water, and sterilize at 121°C for 30 minutes.

[0039] Fermentation medium: in parts by mass, take 10...

Embodiment 2

[0045] Baker's yeast: purchased from Sigam Company.

[0046] Drosophila larvae reared artificially: the feeding conditions are: temperature 29°C, relative humidity 50%, photoperiod L / D=16h / 8h.

[0047] Soil collection: Take the soil under the bayberry fruit tree, remove the topsoil, select the soil within 20cm below the surface, pass through an 80-mesh sieve to remove stones and debris, mix well, and store in a sealed container at 4°C.

[0048] Separation and purification medium: In terms of parts by mass, take 190 parts of potatoes, 22 parts of glucose, 22 parts of agar, and 1000 parts of distilled water, and sterilize at 121° C. for 15 minutes.

[0049] Yeast culture medium: in parts by mass, take 1 part of NaH 2 PO 4 , 1.0 parts (NH 4 ) 2 SO 4 , 0.3 parts MgSO 4 ·7H 2 O, 0.5 part CaCl 2 2H 2 O, mix 1 part of yeast extract, 20 parts of glucose, 1 part of peptone, and 500 parts of water, and sterilize at 121°C for 30 minutes.

[0050] Fermentation medium: in parts b...

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Abstract

The invention relates to the technical field of pesticide, in particular to a preparation method of a drosophila melanogaster attractant. According to the method, ethyl acetoacetate and ethyl chloroacetate are taken as raw materials, a product similar to sex hormone in male drosophila melanogaster is formed through enzymatic reduction reaction, Baker yeast has high reproductivity and relatively high activity, the obtained product is high in purity and activity through enzymatic reaction in the condition, the extraction efficiency is further improved, heterosexual drosophila melanogaster is attracted to be aggregated through the divergence of sex pheromone of drosophila melanogaster, natural enemies are effectively protected and utilized, pollution is reduced, the environment is protected,the method has good efficacy for intensively killing drosophila melanogaster population, the killing base is high, and the control effect is good. The method has the advantages that a sex attractant and pathogenic microbial flora are combined, drosophila melanogaster is firstly aggregated and is then infected and killed biological control microbial strain, chemical residues do not exist during theprocess, and secondary pollution cannot be produced, so that the effect of preventing, treating and controlling drosophila melanogaster is achieved.

Description

technical field [0001] The invention relates to the technical field of pesticides, in particular to a preparation method of a fruit fly attractant. Background technique [0002] At present, the new type of agriculture is in a state of development, and the types and areas of orchards are constantly expanding. However, the fruit fly is a pest of fruit trees. Its living habits make it distributed throughout the tropical and subtropical regions. There is always a large part of fruit in the orchard that suffers from the damage of fruit flies, which makes the fruit growers suffer heavy losses. [0003] Drosophila is a small insect belonging to the family Diptera. It is distributed in all continents of the world and harms fruits and vegetables. There are 253 known varieties, causing huge economic losses to fruit and vegetable farmers all over the world. Fruit flies spread diseases in Africa and endanger human health. [0004] The emergence of fruit flies in the southern provinces...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P19/00A01P7/04
CPCA01N63/30
Inventor 高产明郑薇林凡
Owner 高产明
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