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Application of cinchonain Ib to preparation of medicament for preventing and treating rheumatoid arthritis

A technology for rheumatoid and arthritis, which is applied in the application field of cinchonine Ib in the preparation of drugs for the prevention and treatment of rheumatoid arthritis, and can solve the problems of toxic side effects affecting patient compliance, high price, and difficulty in popularization

Inactive Publication Date: 2018-06-29
天津华泰至成医药科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high price of biological agents, it is difficult to popularize, and the antirheumatic drugs that relieve the disease also seriously affect the patient's compliance due to their toxic side effects

Method used

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  • Application of cinchonain Ib to preparation of medicament for preventing and treating rheumatoid arthritis
  • Application of cinchonain Ib to preparation of medicament for preventing and treating rheumatoid arthritis
  • Application of cinchonain Ib to preparation of medicament for preventing and treating rheumatoid arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Extraction: 5kg fresh Fuji apple pomace black 40L water 80 ℃ reflux extraction 3 times, the extraction time is 3, 2, 1 hour respectively, filter, the mesh number of the filter is 120, the extracts are combined and filtered, filtered The mesh number of the net is 100, and the supernatant is obtained; or select 200g of commercially available apple polyphenols (provided by Tianjin Jianfeng Natural Products Research and Development Co., Ltd., polyphenol content 80%), add 2000mL of water at room temperature and stir to dissolve, set aside ;

[0022] (2) Adsorption and separation of macroporous resin: Adsorb the apple polyphenol solution in (1) through HP20 macroporous resin, and use water and ethanol aqueous solution gradients with volume percentage concentrations of 10%, 30%, 50%, 70%, and 95%. For elution, each half of the column bed volume is used as a receiving volume, and the components rich in cinchonaine Ib are collected by thin-layer chromatography combined with ...

Embodiment 2

[0031] The maximum tolerated dose test and anti-inflammatory activity test of cinchonine Ib.

[0032] 1. Test materials: Animals: ICR mice, 18~22g, both male and female; Wistar rats, weighing 160~190g, male, provided by Beijing Experimental Animal Research Center.

[0033] 2. Reagents: Cinconate Ib (made in the laboratory according to the method in the example, with a purity of 95%); aspirin was purchased from Hunan Yongke Light Industrial Products Import and Export Co., Ltd.; carrageenan was purchased from China Lianxing Industrial Co., Ltd.; Analytical balance, vortex mixer.

[0034] 3. Test method: (1) Maximum tolerance test: divide 40 ICR mice into two groups, group 1, normal water control group; group 2, cinchonaine Ib group. There were 20 mice in each group, half male and half male. After fasting for 12 hours, intragastric administration of cinchonaine Ib with a maximum concentration of 0.2 g / mL was administered to the mice once every 4 hours. Administer 3 times a da...

Embodiment 3

[0046] Analgesic activity test of cinchonaine Ib.

[0047] 1. Test materials: Animals: ICR mice, 18-22g, both male and female; provided by Beijing Experimental Animal Research Center.

[0048] 2. Reagents: Cinchonaine Ib (self-made in the laboratory, Cinchonaine Ib content 95%); Aspirin was purchased from Hunan Yongke Light Industrial Products Import and Export Co., Ltd.; acetic acid was of analytical grade, electronic analytical balance, vortex mixer.

[0049] 3. Test methods: (1) Acetic acid writhing test: 40 mice, half male and half male, were randomly divided into 5 groups, respectively administered with drugs or the same amount of water, and 1 hour after the drug, each mouse was intraperitoneally injected with 0.7% HAC normal saline solution 0.2 ml, observe the number of writhing reactions in mice within 20 min after HAC injection, and calculate the analgesic inhibition percentage.

[0050]

[0051] (2) Mouse hot plate test: test according to Zhao Yi’s method. Fema...

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Abstract

The invention discloses application of cinchonain Ib to preparation of a medicament for preventing and treating rheumatoid arthritis and a healthy food for preventing rheumatoid arthritis. Experimentsshow that the cinchonain Ib can significantly inhibit the auricle swelling of mice caused by xylene and effectively relieve foot swelling of rats caused by carrageenin. An acetic acid abdominal constriction test and a hot plate test show that the cinchonain Ib has a certain analgesic effect, and the acting effect becomes more remarkable along with the acting time. Moreover, the cinchonain Ib hasa good antagonistic effect on primary plantar swelling induced by adjuvants, which indicates that the cinchonain Ib is to have a very good application prospect in the prevention and treatment of rheumatoid arthritis.

Description

technical field [0001] The invention relates to the application of cinchonaine Ib in the preparation of drugs for preventing and treating rheumatoid arthritis. Background technique [0002] Flavonoid lignans are a new class of flavonoids with C-9 substituents, that is, compounds formed by condensation of flavonoids and phenylpropanoid derivatives. Tumor, bactericidal and other biological activities [1] . Cinchonaine Ib is a flavonoid lignan compound, which is derived from the genus Brasilia of Meliaceae, the genus Panax notoginseng in the family Polygonaceae, the genus Panax notoginseng in the family Polygonaceae, the genus Margarita genus in the family Primulaceae, the genus P. The roots, stems, fruits and seeds of plants belonging to 6 families and 8 genera, including the genus Red Sea Lavender, the genus Candela, the genus Smilax in the family Liliaceae, the genus Smilax in the family Rosaceae, the genus Loquat and the genus Apple, are used as raw materials. Current st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P19/02A23L33/105
CPCA61K31/352A23L33/105A23V2002/00A61K36/73A23V2250/21A23V2200/30
Inventor 刘成航王春雷付山
Owner 天津华泰至成医药科技发展有限公司
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