New process for preparing chondroitin sulfate (CS)

A new technology of chondroitin sulfate, applied in the direction of fermentation, etc., can solve the problem of insufficient conversion speed and efficiency in the hydrolysis (enzymolysis) link, which affects the comprehensive quality index of CS products, the yield of finished products, the conversion yield of CS and the conversion efficiency of hydrolysis Conflict and other issues, to shorten the hydrolysis time, promote hydrolysis efficiency, improve purity and quality indicators

Inactive Publication Date: 2018-07-10
连云港中海生物科技有限公司
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AI Technical Summary

Problems solved by technology

[0005] At present, the dilute alkali-enzymatic hydrolysis extraction process mainly has the following shortcomings in industrial practice: (1) The product purity is not high, and the residual polypeptides, proteins, nucleic acids and organic solvents in the crude product will bring great harm to the subsequent purification treatment. (2) The production cycle is too long, on the one hand, the conversion speed and efficiency of the hydrolysis (enzymolysis) link are not enough, and the CS conversion yield and hydrolysis conversion efficiency are two There are conflicts between them; on the other hand, there are various technological difficulties in a series of subsequent treatments (including: alcohol precipitation, filtration, ultrafiltration, oxidation and impurity removal, etc.) based on the hydrolyzate (enzymatic solution), and It takes a lot of work time; (3) In large-scale production, due to the inherent defects of unclear quality and viscous system, the recovery and processing of protein by-products is difficult

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0038] Submerge the pig laryngeal cartilage raw material in water, raise the temperature to 90°C and boil for 5 hours, peel off the muscle tissue attached to the surface of the cartilage, and remove the cartilage; put the boiled cartilage into a reaction kettle at 100°C for 1 hour , the upper layer of fat is collected and sold outside.

[0039] Mix the cartilage obtained above with pure water at a ratio of 1:1; add a mixed enzyme containing alkaline hydrolase and papaya hydrolase at a ratio of 0.40% of the weight of the cartilage (the weight ratio of the two in the mixed enzyme is 1:1), Add the mixture of Pingpingjia O-25 and OP-10 according to 5% of the weight of pure water (the weight ratio of the two in the mixture is 1:1), and add ethanol with a mass fraction of 96% according to 25% of the volume of pure water, at 48 ℃, pH9.5 under the conditions of enzymatic hydrolysis reaction for 1.5h; then add the mixed enzyme containing trypsin and pancreatic lipase according to the p...

Embodiment 2

[0047] Submerge the chicken breast cartilage raw material in water, raise the temperature to 90°C and boil for 4 hours, peel off the muscle tissue attached to the surface of the cartilage, and remove the cartilage; put the cartilage after the step of boiling in a reaction kettle at 100°C for 40 minutes, The upper layer of fat is collected for sale.

[0048] Mix the cartilage obtained above with pure water at a ratio of 1:1; add a mixed enzyme containing alkaline hydrolase and papaya hydrolase at a ratio of 0.45% of the weight of the cartilage (the weight ratio of the two in the mixed enzyme is 1:1), Add the mixture of PEG2000 and OP-10 according to 6% of the weight of pure water (the weight ratio of the two in the mixture is 1:1), add ethanol with a mass fraction of 96% according to 27% of the volume of pure water, at 50°C, pH9. Enzymolysis reaction under the condition of 5 for 1.5h; then add a mixed enzyme containing trypsin and pancreatic lipase according to the proportion o...

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PUM

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Abstract

The invention discloses a new process for preparing chondroitin sulfate (CS), aiming at providing a new process which is simple in technology and high in CS recycling yield, enables the obtained CS finished product to be high in purity as well as convenient and fast in aftertreatment and separation, and facilitates the recycling of protein byproduct. The new process is mainly characterized in thata certain amount of an alcohol ketone polar solvent is added into an enzymatic hydrolysis system in a core proteolytic scheme, and a homogenizing agent is introduced into the enzymatic hydrolysis system. According to the technical scheme provided by the invention, an enzymatic hydrolysis reaction is high in conversion rate and conversion efficiency, and the obtained CS product has the characteristic of high purity; the subsequent treatment of the enzymatic hydrolysate obtained after the enzymatic hydrolysis reaction can be easily and conveniently realized, the operation efficiency of ultrafiltration treatment is greatly increased especially, and a better grading effect is achieved; on the one hand, a high-quality CS product can be obtained by means of a concentrated liquid system, and thehigh-quality protein by-product can also be recovered by means of a liquid permeating system; the traditional oxidation and impurity removal operations do not need to be performed in the process route, the reaction conditions of the overall process are mild, and the operation procedures are simple and easy to implement.

Description

technical field [0001] The invention relates to a new process for preparing chondroitin sulfate, which belongs to the technical field of biochemical industry. Background technique [0002] Chondroitin sulfate (CS) is a type of glycosaminoglycan (GAG) that is covalently linked to proteins to form proteoglycans. Chondroitin sulfate is widely distributed in the extracellular matrix and cell surface of animal tissues. The sugar chain is composed of alternating glucuronic acid and N-acetylgalactosamine (also known as N-acetylgalactosamine) disaccharide units. The chain blocks are linked to serine residues of the core protein. There are various CS production processes reported in the literature, mainly including animal cartilage extraction, microbial fermentation and chemical synthesis. From the point of view of industrial production, extracting CS from animal cartilage is still the most economical and effective method at present. According to this method, commonly used animal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C07K1/14C07K1/34C12P21/06
CPCC07K1/145C07K1/34C08B37/0069C12P21/06
Inventor 万延永
Owner 连云港中海生物科技有限公司
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