Grape seed oil and preparation method thereof
A technology of grape seed oil and grape seed powder, which is applied in the direction of fat oil/fat refining, edible oil/fat, fat oil/fat production, etc. Effect
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Embodiment 1
[0062] Embodiment 1. The influence of mixed enzyme types and additions on the extraction of grapeseed oil
[0063] (1) Grape seed pretreatment: The grape seeds are pulverized by a universal mill and passed through a 50-mesh sieve to obtain the raw material of grape seed powder. %, and the fiber content is 30.6%.
[0064] (2) Enzymatic hydrolysis with mixed enzymes: Mix distilled water and grape seed powder in a volume-to-mass ratio of 5 (mL): 1 (g) to obtain a slurry, add hydrochloric acid to adjust the pH of the slurry to 5, and add mixed enzymes for enzymatic hydrolysis. The hydrolysis reaction temperature was 50 °C, the enzymatic hydrolysis reaction time was 2 h, and the addition scheme of the mixed enzyme was shown in Table 1;
[0065] (3) Acid protease enzymatic hydrolysis: add hydrochloric acid to the slurry, adjust the pH to 4.5, and add acid protease for enzymatic hydrolysis, the addition amount is 0.1%, the enzymatic hydrolysis reaction temperature is 50 ℃, and the e...
Embodiment 2
[0071] Example 2 Effects of protease types and additions on the extraction of grapeseed oil
[0072] (1) Grape seed pretreatment: The grape seeds are pulverized by a universal mill and passed through a 50-mesh sieve to obtain the raw material of grape seed powder. %, and the fiber content is 30.6%.
[0073] (2) Mixed enzyme enzymolysis: Mix distilled water and grape seed powder in a volume-to-mass ratio of 5 (mL): 1 (g) to obtain a slurry, add hydrochloric acid to adjust the pH of the slurry to 5, and add 1% mixed enzyme ( The ratio of cellulase: pectinase: hemicellulase: xylanase is 0.6:0.1:0.2:0.1), the enzymatic hydrolysis reaction temperature is 50°C, and the enzymatic hydrolysis reaction time is 2h;
[0074] (3) Protease hydrolysis: add protease for enzymolysis, the enzymolysis reaction temperature is 50°C, the enzymolysis reaction time is 2h, and the amount of protease added and pH are shown in Table 2.
[0075] (4) lye extraction: sodium hydroxide and distilled water ...
Embodiment 3
[0080] Example 3 Effects of acid protease hydrolysis temperature, time and pH on extraction of grapeseed oil
[0081] (1) Grape seed pretreatment: The grape seeds are pulverized by a universal mill and passed through a 50-mesh sieve to obtain the raw material of grape seed powder. %, and the fiber content is 30.6%.
[0082] (2) Mixed enzyme enzymolysis: Mix distilled water and grape seed powder in a volume-to-mass ratio of 5 (mL): 1 (g) to obtain a slurry, add hydrochloric acid to adjust the pH of the slurry to 5, and add 1% mixed enzyme ( The ratio of cellulase: pectinase: hemicellulase: xylanase is 0.6:0.1:0.2:0.1), the enzymatic hydrolysis reaction temperature is 50°C, and the enzymatic hydrolysis reaction time is 2 h.
[0083] (3) Enzymatic hydrolysis with acid protease: add hydrochloric acid to the slurry, and add acid protease for enzymatic hydrolysis, and the addition amount is 1%. The enzymatic hydrolysis temperature, enzymatic hydrolysis time and pH of acidic proteas...
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