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Indole compounds and application thereof in control of agricultural fungal diseases

A technology of compounds and indoles, applied in the field of chemical agriculture, can solve the problems of less research on the activity of compounds, no literature or reports on agricultural fungicides, etc. Effect

Active Publication Date: 2018-07-20
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are few studies on the activity of this type of compound, so far there is no relevant literature or report on the application of agricultural fungicides in agriculture

Method used

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  • Indole compounds and application thereof in control of agricultural fungal diseases
  • Indole compounds and application thereof in control of agricultural fungal diseases
  • Indole compounds and application thereof in control of agricultural fungal diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Separation, Purification and Structure Confirmation of Compound Indole-3-Acetate Methyl Ester

[0017] Separation and purification method: Carry out liquid culture of the strain, first filter it with gauze to remove mycelia, then use organic solvents with different polarities, petroleum ether, chloroform, ethyl acetate, n-butanol, and the volume ratio of 1.5 for the filtrate: 1 Extract 3 times, concentrate the extract to obtain different polar parts of the fungal fermentation product, extracts from different batches are combined by TLC, and finally perform repeated column chromatography on the different polar extraction parts to separate monomer compounds.

[0018] Structure confirmation: 1H-NMR (600MHz, CD3OD) δ: 7.45 (1H, d, J = 7.9Hz, H-4), 7.27 (1H, d, J = 8.1Hz, H-7), 7.06-7.00 (1H , m, H-6), 6.98-6.92 (1H, m, H-5), 7.08 (1H, s, H-2), 3.69 (2H, s, 2′-H), 3.60 (3H, s, 1'-H). 13C-NMR (150MHz, CD3OD) δ: 174.82 (C-2'), 137.97 (C-8), 128.53 (C-9), 124.63 (C-...

Embodiment 2

[0020] Embodiment 2 compound indole-3-acetic acid methyl ester is to the prevention and treatment of fungal diseases of crops

[0021] Tested strains: Pellicularia filamentosa f.sp.sasakii, Fusarium moniliforme Sheld, Leaf spot fungus, Bacillus wilt, Fusarium wilt, Botrytis cinerea, Target spot fungus, Alternaria, etc. All provided by the Institute of Plant Protection.

[0022] The test drug compound indole-3-acetic acid methyl ester was isolated and purified from bacteria, and after broad-spectrum identification, it was dissolved in deionized water to make a 200 μg / ml mother solution, and stored at 4°C for future use. PDA medium (potato powder 3.0g, glucose 20.0g, agar 14.0g): Weigh 37g of this product, add 1000ml of distilled water, heat and boil to dissolve, aliquot, autoclave at 121℃ for 15min, and set aside.

[0023] The mycelium growth rate method was used to measure the inhibition of mycelial growth. The bacteria (diameter 5mm) was picked from the edge of the pre-culti...

Embodiment 3

[0029] Embodiment 3: the control of rice sheath blight

[0030] Test method: Spray once at the early stage of disease onset, and treat 3 plots for each plot, each plot is 20 square meters. Investigate and count the incidence of disease before and 10 days after the drug, randomly sample at 5 points in each plot, investigate the percentage of the area of ​​the sheath blight on each leaf on the whole plant to the leaf area, and calculate the disease index and control effect.

[0031] Preparation method: Weigh 27% methyl indole-3-acetate, 6% calcium dodecylbenzenesulfonate, 7% sodium lignosulfonate, 35% white carbon black, 25% attapulgite, mix them, and airflow Wettable powder can be obtained after crushing. The preparation prepared according to the above ratio is diluted 50 times and sprayed, and the control effect on rice sheath blight is more than 70%.

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Abstract

The invention discloses indole compounds and an application thereof in control of agricultural fungal diseases and belongs to the technical field of chemical agriculture. The compounds are mainly usedfor control of the fungal diseases and can effectively control fungal diseases of plants, such as banded sclerotial blight, bakanae diseases, bipolaris oryzae, gummy stem blight, wilt, botrytis cinereal, target leaf spot, alternaria alternata and the like. An agricultural fungal disease bactericide prepared from the compounds as an active ingredient has the characteristics of good control effect,wide spectrum, low toxicity, no residues, no pollution and no harm and is particularly beneficial to environment protection. Therefore, some bactericides can be prepared from the compounds to protectagriculture from invasion by fungal diseases.

Description

technical field [0001] The invention belongs to the technical field of chemical agriculture, and in particular relates to an indole compound and its application in the prevention and treatment of agricultural fungal diseases. Background technique [0002] Diseases on crops are not easily detected by people in time like pests and weeds, so they often cause neglect and difficulties in prevention and control, and the damage caused is even more serious. For example, several major fungal diseases such as sheath blight, bakanae disease, flax leaf spot, vine blight, fusarium wilt, gray mold, target spot and Alternaria on crops occur every year, causing crops A large amount of production is reduced and its quality is affected, which brings great economic losses to farmers. According to reports, fungal diseases on crops have a great impact on their yield, and the annual loss of yield is about 20% to 30% of the total yield. [0003] In order to meet people's increasing demand for fo...

Claims

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Application Information

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IPC IPC(8): C07D209/18A01N43/38A01P3/00
CPCA01N43/38C07D209/18
Inventor 折冬梅刘万礼
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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