Fluorescence staining reagent for marking leucorrhoea and cervical exfoliated cell pathogenic bacteria

A technology of exfoliated cells and fluorescent staining, which is applied in the field of biomedical diagnosis, can solve the problems of low positive rate, unintuitive enzymatic reaction results, slow growth of fungi, etc., achieve high sensitivity and positive rate, solve mixed infection, clinical Ease of use

Inactive Publication Date: 2018-07-31
江苏诺鬲生物科技有限公司
View PDF5 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Disadvantages: low positive rate, atypical morphology easily leads to missed diagnosis, large subjective error of inspectors
Disadvantages: Immediate detection of vaginal secretions is required, otherwise the enzyme will lose its activity; the detection process often requires incubation at 37°C, the operation process is a bit cumbersome, and the enzymatic reaction results are not intuitive, which is likely to cause false positives
Disadvantages: Although the detection results of the culture method are highly accurate, due to the slow growth of fungi, the culture often takes days or even

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescence staining reagent for marking leucorrhoea and cervical exfoliated cell pathogenic bacteria
  • Fluorescence staining reagent for marking leucorrhoea and cervical exfoliated cell pathogenic bacteria
  • Fluorescence staining reagent for marking leucorrhoea and cervical exfoliated cell pathogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation of Gynecological Fluorescence Staining Reagent

[0030] Reagent 1: Liquid A configuration: Dissolve fluorescent whitening agent 28 in a solution with a concentration of 1.0% (W / V) potassium chloride, and dissolve sufficiently so that the concentration of fluorescent whitening agent dye is 0.2% (W / V) , add Evans blue dye and dimethyl sulfoxide, the concentration of Evans blue dye is 0.2% (W / V), and the concentration of dimethyl sulfoxide is 20% (V / V). Solution B configuration: Dissolve acridine orange in water so that the concentration of acridine orange is 0.1% (W / V).

[0031] Reagent 2:

[0032] Solution A configuration: Dissolve the fluorescent whitening agent 28 in a solution with a concentration of 1.0% (W / V) potassium chloride, and dissolve sufficiently so that the concentration of the fluorescent whitening agent 28 is 0.2% (W / V). Evans blue dye, dimethyl sulfoxide, glycerol and β-D-glucan binding protein, goat anti-rabbit polyclonal antibod...

Embodiment 2

[0033] Example 2 Staining comparison of leucorrhea specimen

[0034] Select 3 leucorrhea specimens infected by pathogenic bacteria, place them on glass slides, stain with reagents 1 and 2 for 2 minutes, add a cover glass, absorb the excess dye with paper, and then observe under a fluorescent microscope . View the fungus under the UV band and the fungus will appear blue or blue-green. Then switch the B band to observe bacteria and trichomonas, the bacteria are displayed in orange, and the bacteria are observed to adhere to the clue cells. The trichomonad body is marked in red, and there is a specific slash yellow nuclear morphology in 1 / 3. The results showed that both reagents could stain the pathogenic bacteria in leucorrhea specimens. But the effect of reagent 1 is poor, and the color development time is short, while the effect of reagent 2 is good, and the color development time is long.

[0035] Table 1

[0036]

Embodiment 3

[0037] Embodiment 3 solution stability investigation

[0038]Reagent 1 and reagent 2 were left at room temperature for 6 months, and then the leucorrhea specimens were stained. The stability of the reagent was judged from the staining effect. The results are shown in Table 2.

[0039] Table 2

[0040]

[0041] The results showed that after being placed at room temperature for 6 months, the appearance and color of reagent 1 and reagent 2 remained unchanged, and there was no precipitation and crystallization phenomenon, and the reagents remained stable. Reagent 1 stained poorly, while Reagent 2 performed well.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a fluorescence staining reagent for marking leucorrhoea and cervical exfoliated cell pathogenic bacteria. The reagent comprises reagent liquid A and reagent liquid B, wherein the liquid A comprises water, salt, binding protein, specific antibody, fluorescent whitening agent, dissolution promoter, wetting agent and background counterstain, the liquid B comprises water and fluorescein. The fluorescence staining reagent for marking leucorrhoea and cervical exfoliated cell pathogenic bacteria has the advantages that the operation is simple, the staining can be completed just by two operation processes; the staining time is short, observation can be conducted within one minute; compared with a traditional microscopy method, the sensibility and the positive rate are higher; compared with a dry chemical ferment method, the result is more visual and accurate, and the situation of mixed type infection can be better solved, the clinical application is more convenient andquicker, and the diagnostic efficiency of vaginitis and cervicitis is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of biomedical diagnosis, and in particular relates to a fluorescent staining reagent for marking leucorrhea and cervical exfoliated cell pathogenic bacteria. Background technique [0002] Vaginitis is a common gynecological disease caused by pathogenic bacteria in clinical practice. According to the different types of pathogenic bacteria, it can be divided into the following three types: bacterial vaginosis, candida vaginitis, and trichomonas vaginitis. Patients with bacterial vaginosis mainly present with increased vaginal discharge with a fishy smell, especially after sexual intercourse, and may be accompanied by mild genital itching or burning sensation. It is mainly caused by endogenous mixed infection caused by the decrease of Lactobacillus in the vagina and the increase of Gardnerella and anaerobic bacteria. Candidal vaginitis manifests as vulvar erythema, edema, and white membranes. The secretion is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N1/30G01N21/64
CPCG01N1/30G01N21/6428G01N2001/302G01N2021/6439
Inventor 何丹
Owner 江苏诺鬲生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap