Method for extraction and enrichment of cysteine-containing peptides based on fluorine solid phase and mass spectrometry

Abstract

The invention belongs to the field of analytical chemistry and protein analysis and relates to a novel method for fluorine solid phase extraction and particularly relates to a method for separating and enriching cysteine-containing peptides by fluorinated graphite as a solid phase adsorbent. Through use of fluorinated graphite as a solid phase adsorbent for fluorine solid phase extraction and thefluorine-fluoride action between the fluorinated graphite surface and the target analyte fluorine label, the target analyte and impurities are effectively separated. Compared with the conventional fluorine solid phase extraction adsorbent, the fluorinated graphite prevents the use of a high-purity silica matrix and a highly fluorinated silane coupling agent. The method has the characteristics of low cost, good selectivity, low detection limit, high recovery rate and high popularity and can realize separation, enrichment and mass spectrometry identification of cysteine-containing peptides.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and protein analysis, and relates to a new method of fluorine solid-phase extraction, in particular to a fluorine solid-phase extraction technology using graphite fluoride as a solid-phase adsorbent, and using the technology to separate and enrich proteolytic peptides Method for cysteine ​​peptides. Background technique [0002] The prior art discloses that fluorine solid phase extraction is a widely used technique in organic synthesis and catalysis. This technology derivatizes the target compound with a fluorine reagent, utilizes fluorine-fluorine interaction, and relies on highly fluorinated solid-phase adsorbents to separate and purify the target compound. Practice has shown that compared with traditional biotin tags, fluorine tags are more compatible with mass spectrometry, so in recent years it has been used in various "omics" research (such as proteomics, glycomics, metabolomics) have b...

AI Technical Summary

Problems solved by technology

Studies have shown that with the development of organic fluorine chemistry, there are a wide variety of derivative reagents with fluorine labels, which greatly promotes the development of fluorine solid-phase extraction technology. As a matrix, it is made by bonding a highly fluorinated silane coupling agent, and its cost is relatively high, which limits the further popularization of fluorine solid phase extraction technology

Fluorinated graphite is a kind of cheap and easy-to-obtain high-fluorinated materials, which are often used as solid-phase lubricants and lithium battery cathode materials in industry, but fluorinated graphite materials have not been used as solid-phase adsorption for fluorine solid-phase extraction. agent reports

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