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Composition, containing lithospermi radix extract as active ingredient, for preventing, alleviating, or treating peripheral neuropathy

A technology for peripheral neuropathy and active ingredients, which is applied in the field of compositions containing comfrey root extract as an active ingredient for preventing, improving or treating peripheral neuropathy, and can solve problems such as no technology for peripheral neuropathy reported for comfrey roots, To achieve the effect of reducing sensitivity and alleviating inhibition

Inactive Publication Date: 2018-07-31
KOREA INST OF ORIENTAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The drug is known to be used as a purple dye, or to lower body temperature, purify blood, and improve blood circulation, and Korean Patent No. 0558159 discloses health using Lithospermi Radix root which has excellent effects on recovery from fatigue and child development Preparation method of supplementary food, Korean Laid-open Patent No. 2015-0047173 discloses a composition containing comfrey extract as an active ingredient for preventing or improving osmotic inflammation of the lungs caused by acute pancreatitis metastasis, but has not yet reported Peripheral Neuropathy Related Techniques of Comfrey Root

Method used

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  • Composition, containing lithospermi radix extract as active ingredient, for preventing, alleviating, or treating peripheral neuropathy
  • Composition, containing lithospermi radix extract as active ingredient, for preventing, alleviating, or treating peripheral neuropathy
  • Composition, containing lithospermi radix extract as active ingredient, for preventing, alleviating, or treating peripheral neuropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0143] Embodiment 1. The HPLC index analysis of comfrey root extract

[0144] Using the comfrey root extract (WLR) prepared according to [Material and Method 1], HPLC analysis was carried out according to [Material and Method 2]. When comparing the retention time (retention time, RT) and UV spectrum of the components contained in the comfrey root extract and the standard, five peaks (peaks) ( figure 1 ). The five confirmed peaks are: rosmarinic acid with RT value of 29.745 minutes, lithospermic acid with RT value of 30.101 minutes, salvianolic acid B with RT value of 31.662 minutes B), salvianolic acid A (salvianolic acid A) with RT value of 33.772 minutes, and salvianolic acid C (salvianolic acid C) with RT value of 38.551 minutes.

Embodiment 2

[0145] Example 2. In vitro (in vitro) confirmation of the neuroprotective effect of the comfrey root extract

[0146] 2-1. Neuroprotective effect of comfrey root extract - qualitative analysis

[0147] The neuroprotective effect of the comfrey root extract of the present invention was confirmed using a neurite outgrowth assay kit (NS225, Millipore) using a membrane with a pore of 1 μm (membrane).

[0148] Specifically, DMEM low-serum differentiation medium containing 1% horse serum, 100 units / ml of penicillin, 100 μg / ml of streptomycin, and 100 ng / ml of nerve growth factor (NGF) was used before the experiment. (lowerum differentiation media) PC-12 cells cultured according to [Materials and methods 3-1] were cultured for 72 hours, and membranes with pores of 1 μm were soaked in 400 μl / well containing 10 μg / ml collagen Protein solutions were coated in 24-well plates at 37 °C for 2 hr. The coated membrane was transferred to a well containing 600 µl of a differentiation medium,...

Embodiment 3

[0154] Example 3. Confirmation of CIPN inhibitory effect of comfrey root extract in vivo

[0155] The CIPN-inhibiting effect of the comfrey root extract of the present invention was confirmed by measuring mechanical allodynia in an animal model of oxaliplatin-induced peripheral neuropathy.

[0156] Specifically, oral administration of 250 mg / kg of comfrey root extract prepared according to [Material and Method 1] was started 2 weeks after the induction of pain in the animal model of peripheral neuropathy prepared according to [Material and Method 4]. (WLR), or 0.5% carboxymethylcellulose (carboxymethyl cellulose, CMC) as negative control group, administered 6 times per week, used 4 weeks altogether; As positive control group, intraperitoneal administration of 100mg / kg amifostine , administered twice a week for a total of 4 weeks. From 2 weeks after the induction of the pain sensation, a von Frey filament test in which 0.4 g of the same stimulus was applied to the hind paw (hi...

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Abstract

The present invention relates to a composition, containing a Lithospermi Radix extract as an active ingredient, for preventing, alleviating, or treating peripheral neuropathy, especially, chemotherapy-induced peripheral neuropathy (CIPN). Specifically, it was verified that the Lithospermi Radix extract of the present invention causes no toxicity in normal cells, does not influence an intrinsic cytotoxic effect of an anticancer drug in a human cancer cell line, mitigates the inhibition of neurite growth due to an anticancer drug, and effectively reduces the sensitivity to increased pain stimulation in a chemotherapy-induced peripheral neuropathy animal model, and thus the Lithospermi Radix extract can be favorably used as an active ingredient of the composition for preventing, alleviating,or treating chemotherapy-induced peripheral neuropathy.

Description

technical field [0001] The present invention relates to a composition for preventing, improving or treating peripheral neuropathy, which contains Lithospermi Radix root extract as an active ingredient. Background technique [0002] Peripheral neuropathy is caused by diseases that directly damage or affect nerve tissue. According to the type of nerve tissue affected, it can be divided into sensory neuropathy and motor neuropathy. (motor neuropathy), autonomic neuropathy (autonomic neuropathy). In the case of sensory neuropathy, tactile numbness and shaking phenomena are felt, the sensation of temperature changes is decreased, or tingling or burning sensations are felt, and allodynia is felt on the skin, etc. Motor neuropathy is accompanied by a weakened sense of balance and muscle strength. Autonomic neuropathy, depending on the organ affected by the nerve, weakens the function of regulating the bladder, leading to urinary incontinence, or causing Abnormal blood pressure an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/30A61K31/282A61K31/337
CPCA61K31/282A61K31/337A61K36/30A61P25/02A61K2300/00
Inventor 金老洙朴钟植李镇武李裕珍赵恩常金镇喜林采俊方玉仙金荣阿
Owner KOREA INST OF ORIENTAL MEDICINE
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