Applications of pinoresinol in preparing products for preventing and treating diseases induced by oxidative stress

A technology of oxidative stress and pinoresinol, which is applied in the field of medicine to achieve the effect of increasing GSH levels and inhibiting cell damage and apoptosis

Inactive Publication Date: 2018-08-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cinnamon contains sesquiterpenoids, phenolic acids, lignans, flavonoids, flavanols, phenylpropanoids, coumarins, steroids, alpha, beta -Unsaturated-γ-butyrolactone compounds and other compounds, among which phenylpropanoid compounds (especially

Method used

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  • Applications of pinoresinol in preparing products for preventing and treating diseases induced by oxidative stress
  • Applications of pinoresinol in preparing products for preventing and treating diseases induced by oxidative stress
  • Applications of pinoresinol in preparing products for preventing and treating diseases induced by oxidative stress

Examples

Experimental program
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preparation example Construction

[0044] The preparation and structural confirmation of embodiment pinoresinol

[0045] The obtaining method of pinoresinol is as follows: the cinnamon medicinal material is extracted with 95% ethanol to obtain an ethanol extract, and then sequentially extracted with petroleum ether, ethyl acetate and n-butanol. The ethyl acetate part was eluted with a petroleum ether-ethyl acetate system gradient, and the eluent concentrations were: petroleum ether-ethyl acetate=100:0, petroleum ether-ethyl acetate=95:5, petroleum ether-ethyl acetate Ester=90:10, petroleum ether-ethyl acetate=85:15, petroleum ether-ethyl acetate=80:20, petroleum ether-ethyl acetate=70:20, petroleum ether-ethyl acetate=60:40, Petroleum ether-ethyl acetate=50:50, after combining, 8 fractions (Fr.1-8) were obtained. Fr.4 (petroleum ether-ethyl acetate=85:15 eluted fraction) was eluted through a silica gel column with petroleum ether / ethyl acetate (90:10→50:50) as the mobile phase gradient, and a total of 10 fract...

Embodiment 2

[0048] Example 2: Evaluation of NQO1-inducing activity of pinoresinol

[0049] (1) Culture of mouse hepatoma cell line Hepa 1c1c

[0050] The mouse hepatoma cell line Hepa 1c1c was purchased from the American Type Culture Collection (ATCC), using MEM medium containing 10% fetal bovine serum (FBS), placed at 37 °C, 5% CO 2 cultured in an incubator.

[0051] (2) NQO-inducing activity test

[0052] Hepa 1c1c cells were inoculated on a 96-well plate, and different concentrations of pinoresinol (confirmed in Example 1) were added after the cells adhered to the wall for 24 hours. The cells were lysed with 0.8% digitonin solution, and the detection solution (1.0 mL 0.5 M Tris Hydroxymethylaminomethane-hydrochloric acid (Tris-HCl), 15 mg bovine serum albumin, 6 mg MTT, 150 μL Tween-20, 150 μL 150 mM D-glucose-6-phosphate, 15 μL 7.5 mM flavin adenine dinucleotide, 27 μL 50mM nicotinamide adenine dinucleotide phosphate, 20 μL 50mM menadione), let stand for 3min, and measure the lumin...

Embodiment 3

[0054] Example 3: pinoresinol can up-regulate the protein levels of Nrf2, γGCS and NQO1

[0055] Method: Western blot analysis (Western blot) to detect changes in protein levels in cells

[0056] Beas-2B cells were inoculated in a 35 mm diameter petri dish, cultured until the density reached 70%-80%, and then treated with different concentrations of compounds to be tested for 16 h, washed twice with PBS, added cell lysate (50 μg / ml aprotinin , 0.5mM phenylmethylsulfonyl fluoride, 1mM sodium orthovanadate, 10mM sodium fluoride, 10mMβ-glycerol phosphate), the protein was collected and the protein concentration was determined by Bradford method. Each sample protein (100 μg) was loaded on the sample, and the protein components were separated by SDS-PAGE, and the protein bands were transferred to the nitrocellulose membrane by electrotransfer method. After the film was sealed with 5% skimmed milk powder solution prepared in TBS at room temperature for 1 hour, it was incubated with...

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Abstract

The invention discloses applications of pinoresinol in preparing products for preventing and treating diseases induced by oxidative stress. The chemical formula of pinoresinol is shown in the description, for the pinoresinol, by activating the Nrf2 signal channel, the protein levels of the antioxidant enzyme gamma GCS and the II phase detoxification enzyme NQO1 can be up-regulated, the GSH level of the endogenous antioxidant of cells is increased, and the cellular injuries induced by oxidative stress are inhibited; the pinoresinol has the similar cell protection effects for the human bronchusepithelial Beas-2B cells, the human renal glomerular endothelial cells HRGEC cells, the human nerve SHSY5Y cells and the human breast cancer MDA-MB-231 cells. The lignans can be used for preparing health products and medicines for preventing or treating diseases induced by oxidative stress.

Description

technical field [0001] The invention belongs to the field of medicine and relates to the application of lignan compounds, in particular to the application of a lignan compound in the preparation of medicines or health care products for preventing or treating oxidative stress-induced diseases. Background technique [0002] Chronic non-communicable diseases (referred to as chronic diseases) have become the main cause of death and disability worldwide. Common chronic diseases include: diabetes, hypertension, cardiovascular disease, chronic obstructive pulmonary disease, tumors, etc. These diseases have a common feature - oxidative stress plays an important role in their pathogenesis. Oxidative stress is an imbalance between oxidation and anti-oxidation in the body, and tends to be oxidized. The main feature is the increase in the level of reactive oxygen species and reactive nitrogen. chronic. In daily life, common oxidative stress-inducing factors include: environmental fact...

Claims

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Application Information

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IPC IPC(8): A61K31/34A61K9/20A61P11/00A61P13/12A61P3/10A61P25/00A61P25/28A61P35/00
CPCA61K9/2059A61K31/34A61P3/10A61P11/00A61P13/12A61P25/00A61P25/28A61P35/00
Inventor 沈涛李爱玲任冬梅王小宁娄红祥
Owner SHANDONG UNIV
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