Silk fibroin hydrogel

Abstract

The invention relates to a hydrogel material, and in particular relates to silk fibroin hydrogel; the silk fibroin hydrogel provided by the invention is the silk fibroin hydrogel prepared from a silkfibroin solution under the action of an inducer containing large molecular weight polyethylene glycol, wherein the large molecular weight polyethylene glycol refers to polyethylene glycol having a weight average molecular weight of 3,000 to 8,000; the silk fibroin hydrogel provided by the invention has good biocompatibility, can promote cell proliferation, is matched with natural tissue in mechanical strength, injectable, and easy to implant, and has no toxic and side effects.

Description

technical field [0001] The invention relates to a hydrogel material, in particular to a silk fibroin hydrogel Background technique [0002] For the treatment of defective or unsightly tissues (such as from trauma, surgical resection or congenital defects), there are two types of tissue sources: autologous tissue transplantation and artificial filling; from the implantation method, it can be divided into window implantation and implantation. Injection fills both. Autologous tissue is limited by sources and is difficult to be widely used, and it will cause secondary trauma to the injured. Artificial filling materials are more and more widely used in clinical practice; injection filling, due to its small wound, simple operation, and less pain for patients, etc. It is widely used in the fields of filling and cosmetic plastic surgery after soft tissue trauma or surgical resection. Therefore, the research and development of injectable soft tissue filling materials has become a r...

AI Technical Summary

Problems solved by technology

[0009] The invention patent of Chinese Patent Publication No. CN 106267370 A discloses a silk fibroin / cellulose 3D printing ink using genipin as a cross-linking agent. Although the article discloses that the material can be used to embed cells, however Tests have proved that genipin has poor biocompatibility, resulting in insufficient overall bioidentity of the material, which cannot be used for embedding cells, and its chemical cross-linking is complex, and its biological safety is yet to be confirmed

[0010] The Chinese Patent Publication No. CN 106362208 A discloses a silk fibroin / xanthan gum hydrogel, which uses silk fibroin powder instead of regenerated silk fibroin. Cross-linking does not form cross-linking between silk fibroin. At this time, silk fibroin powder is no different from other particles that can be cross-linked, and cannot reflect the good biocompatibility of silk fibroin. At the same time, silk fibroin powder The surface is hydrophobic, which is not conducive to cell adhesion and growth

[0011] Chinese Patent Publication No. CN 103289107 A invention patent discloses a silk fibroin in situ gel, which uses a single small molecular weight polyethylene glycol to induce silk fibroin gel, but the low molecular weight polyethylene glycol has a high osmotic pressure , causing the silk fibroin gel to inhibit cell adhesion and proliferation

[0012] The Chinese publication number is CN 101772348 B, which uses ultrasonic method to prepare silk fibroin gel. The silk fibroin hydrogel is not injectable, and when the silk fibroin concentration is higher than 4%, the cells cannot proliferate normally.

Images