Silk fibroin hydrogel

A technology of silk fibroin and hydrogel, which is applied in the fields of medical science, prosthesis, tissue regeneration, etc., can solve the problem of poor biocompatibility, high osmotic pressure of small molecular weight polyethylene glycol, and inability to reflect the biological phase of silk fibroin. Capacitance and other issues

Inactive Publication Date: 2018-08-17
SUZHOU SIMEITE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The invention patent of Chinese Patent Publication No. CN 106267370 A discloses a silk fibroin/cellulose 3D printing ink using genipin as a cross-linking agent. Although the article discloses that the material can be used to embed cells, however Tests have proved that genipin has poor biocompatibility, resulting in insufficient overall bioidentity of the material, which cannot be used for embedding cells, and its chemical cross-linking is complex, and its biological safety is yet to be confirmed
[0010] The Chinese Patent Publication No. CN 106362208 A discloses a silk fibroin/xanthan gum hydrogel, which uses silk fibroin powder instead of regenerated silk fibroin. Cross-linking does not form cross-linking between silk fibroin. At this time, silk fibroin powder is no different from other particle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0039] Example one

[0040] This embodiment provides an experimental process and results of the effect of polyethylene glycol of different molecular weights on cell proliferation, which are specifically as follows:

[0041] S1. P2 human bone marrow mesenchymal stem cells are re-thawed and frozen, inoculated into T175 cell culture flasks, and cultured in DMEM medium containing 10% FBS and 1% double antibody. When the cells are expanded to cover 80% of the bottom of the culture flask, wash twice with PBS, digest with 2mL 0.25% trypsin, and add 4mL cell culture solution to terminate the digestion. Centrifuge, resuspend in cell culture medium, and count.

[0042] S2, 5000 cells / well were seeded in a 24-well cell culture plate, and then cultured in DMEM medium containing 10% FBS and 1% double antibody for 12 hours.

[0043] S3. Discard the supernatant, add a culture solution containing 5% polyethylene glycol with different molecular weights and a mass concentration of 5%, and continue cul...

Example Embodiment

[0054] Example two

[0055] This example provides a silk fibroin hydrogel prepared by polyethylene glycol of different molecular weights and combinations thereof, and the three-dimensional structure of the hydrogel is studied using scanning electron microscope to detect the effect of polyethylene glycol of different molecular weight on silk fibroin. The influence of protein gel structure. Specific steps are as follows:

[0056] S1. Cut the silkworm cocoon silk into small pieces and weigh 100 grams, boil it in 40L sodium carbonate aqueous solution with a molar concentration of 0.02M for 30 minutes to remove sericin, and then repeatedly scrub with deionized water for 3 times. After washing, put it in a fume hood Dry and set aside.

[0057] S2. Weigh 10 grams of degummed silk and place it in 40 mL of 9.3M lithium bromide solution at 60°C for 4 hours. Place in a dialysis device for dialysis against ionized water for 48 hours, and centrifuge to remove insoluble particles.

[0058] S3. D...

Example Embodiment

[0070] Example three

[0071] This embodiment provides a silk fibroin solution that simultaneously acts on large molecular weight polyethylene glycol and simultaneously adds silk fibroin promoted by small molecular weight polyethylene glycol to form a hydrogel. The comparison of the results of the gel formation speed includes the following step:

[0072] S1. Cut the silkworm cocoon silk into small pieces and weigh 100 grams, boil it in 40L sodium carbonate aqueous solution with a molar concentration of 0.02M for 30 minutes to remove sericin, and then repeatedly scrub with deionized water for 3 times. After washing, put it in a fume hood Dry and set aside.

[0073] S2. Weigh 10 grams of degummed silk and place it in 40 mL of 9.3M lithium bromide solution at 60°C for 4 hours. Place in a dialysis device for dialysis against ionized water for 48 hours, and centrifuge to remove insoluble particles.

[0074] S3. Add large molecular weight polyethylene glycol to the silk fibroin solution o...

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Abstract

The invention relates to a hydrogel material, and in particular relates to silk fibroin hydrogel; the silk fibroin hydrogel provided by the invention is the silk fibroin hydrogel prepared from a silkfibroin solution under the action of an inducer containing large molecular weight polyethylene glycol, wherein the large molecular weight polyethylene glycol refers to polyethylene glycol having a weight average molecular weight of 3,000 to 8,000; the silk fibroin hydrogel provided by the invention has good biocompatibility, can promote cell proliferation, is matched with natural tissue in mechanical strength, injectable, and easy to implant, and has no toxic and side effects.

Description

technical field [0001] The invention relates to a hydrogel material, in particular to a silk fibroin hydrogel Background technique [0002] For the treatment of defective or unsightly tissues (such as from trauma, surgical resection or congenital defects), there are two types of tissue sources: autologous tissue transplantation and artificial filling; from the implantation method, it can be divided into window implantation and implantation. Injection fills both. Autologous tissue is limited by sources and is difficult to be widely used, and it will cause secondary trauma to the injured. Artificial filling materials are more and more widely used in clinical practice; injection filling, due to its small wound, simple operation, and less pain for patients, etc. It is widely used in the fields of filling and cosmetic plastic surgery after soft tissue trauma or surgical resection. Therefore, the research and development of injectable soft tissue filling materials has become a r...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/22A61L27/26A61L27/52
CPCA61L27/18A61L27/227A61L27/26A61L27/52A61L2400/06A61L2430/34C08L89/00C08L71/02
Inventor 王晓沁郑兆柱
Owner SUZHOU SIMEITE BIOTECH CO LTD
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