Preparation method and application of extract of Galla chinensis

Abstract

The invention discloses a preparation method and application of extract of Galla chinensis. Specifically, the Galla chinensis is pulverized into powder, the obtained powder is dispersed in a hydrochloric acid solution, and dissolution of active ingredients in the plant is accelerated through a cavitation effect of ultrasonic waves; extraction experiments are carried out by adopting the concentration of hydrochloric acid, times of the quantity of a solvent, the extraction time and the extraction frequency as single factor levels, and optimization is performed by using an orthogonal table; thena concentration gradient of the extract of Galla chinensis is prepared so as to form Galla chinensis antibacterial agents of different concentrations; inhibitory effects of the Galla chinensis antibacterial agents of different concentrations on Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio vulnificus and other vibrio are observed by using a K-B paper-disc agar diffusion method. The growthof the Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio vulnificus and other aquatic pathogenic bacteria can be inhibited to a minimum extent through the Galla chinensis antibacterial agent withthe best antibacterial effect, and therefore the yield of Penaeus vannamei and some other aquatic products is improved greatly, so that greater economic benefits are brought to cultivators, and the economic development of China is further promoted.

Description

technical field [0001] The invention relates to the technical fields of plant extracts, microbial culture, aquaculture and the like, in particular to a preparation method and application of a gallnut extract. Background technique [0002] The main component of gallnut is Tannin Acid, also known as tannin, tannic acid or tannin. In vitro experiments show that the gall extract has obvious bacteriostatic or bactericidal effects on Staphylococcus aureus, Streptococcus, Pneumococcus, Typhoid Bacillus, Paratyphoid Bacillus, Shigella, Bacillus anthracis, Diphtheria, Pseudomonas aeruginosa, etc. Clinical studies have shown that gall extract combined with gentamicin and metronidazole can enhance the bactericidal effect, especially on Pseudomonas aeruginosa and anaerobic bacteria. strong inhibitory effect. Due to the important role of gall extract in antibacterial, the preparation method of gall extract has become a research hotspot. [0003] Existing preparation methods of gallnut...

AI Technical Summary

Problems solved by technology

However, with the continuous expansion of breeding scale and the increase of stocking density in recent years, the diseases caused by bacterial and viral diseases have become increasingly serious, especially the impact of Vibrio, which has caused serious economic losses to farmers and has become the current Bottlenecks restricting the sustainable development of Litopenaeus vannamei aquaculture

At present, antibiotics, amines, quinolones and other chemically synthesized drugs are mainly used for the prevention and treatment of infectious diseases of Litopenaeus vannamei. These drugs have brought about a series of problems such as drug resistance, drug residues and environmental pollution during the long-term use. , many countries and regions have successively made decisions in the form of legislation to prohibit or restrict the addition of chemically synthesized drugs to aquaculture feed

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