A kind of monolithic material of phospholipid organic polymer and its preparation method and application

A technology of monolithic materials and polymers, applied in the field of protein purification, can solve the problems of incompatibility between specificity and recovery, lack of specificity, reproducibility, and purity to be further verified
CN108456280BActive Publication Date: 2020-10-27JINAN UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JINAN UNIVERSITY
Publication Date
2020-10-27

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Abstract

The invention belongs to the technical field of protein purification, and discloses a phospholipid organic polymer whole material and a preparation method and application thereof. The method comprisesthe steps that phospholipid monomeric compound, crosslinking agent, porogen and initiator are mixed and then poured into a container after ultrasonic dissolving and deaeration are conducted, aggregation reaction is conducted under the temperature of 40-70 DEG C or under ultraviolet irradiation, and the phospholipid organic polymer whole material is obtained. The phospholipid organic polymer wholematerial has the advantages of high reproducibility, good biocompatibility and protein nonspecific adsorption resistance, and the existing defects of a traditional CRP purified material are effectively overcome. According to the material, by taking the phospholipid organic polymer whole material as an absorbent purifying standard protein mixed sample or an actual sample, CRP proteins with high purity can be obtained simply by one purifying strategy, the operation is easy, and the recovery rate is close to 100%.
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Description

technical field

[0001] The invention belongs to the technical field of protein purification, and in particular relates to a phospholipid organic polymer integral material, a preparation method and application thereof. Background technique

[0002] In 1930, Tillett and Francis discovered that in patients with pneumonia, there is a substance that can react with the phospholipid components on the cell wall of Streptococcus pneumoniae C polysaccharide (CPS) to produce a precipitate [W.S.Tillett,, T.Jr.Francis, J.Exp .Med.,1930,52:561], later called C-reactive protein (CRP). Native CRP is a non-covalent pentameric structure composed of 5 independent subunits, produced by hepatocytes, and mainly present in serum, ascites and cerebrospinal fluid [J.P.Atkinson,Arthritis Rheum.,2001,44:995– 996.], its content is very low in the normal body, and its content will increase hundreds of times during inflammatory reactions, so it has become a non-specific acute phase protein [M.McCarty, J...

Claims

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