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Preparation method of composite small-caliber artificial blood vessel, and product thereof

An artificial blood vessel and small-caliber technology, which is applied to the human tubular structure devices, blood vessels, packaged food, etc., can solve the problems of accelerated elastin degradation, reduced blood vessel mechanical properties, low patency rate, etc., to promote adhesion and increase mechanical properties. , the effect of facilitating reconstruction

Inactive Publication Date: 2018-09-04
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the vascular material is decellularized, the endothelial cells on the inner wall of the blood vessel are removed, and the subintimal collagen is exposed, which can activate the coagulation system. Therefore, if the decellularized blood vessel is directly used as a substitute material for implantation in the body, it is easy to cause thrombus, and its patency The rate is not high, and after decellularization, the mechanical properties of blood vessels are reduced, and the degradation of elastin is accelerated, which leads to the formation of aneurysms.

Method used

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  • Preparation method of composite small-caliber artificial blood vessel, and product thereof
  • Preparation method of composite small-caliber artificial blood vessel, and product thereof
  • Preparation method of composite small-caliber artificial blood vessel, and product thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1, the preparation method of composite small-diameter artificial blood vessel

[0026] The preparation method of composite small-diameter artificial blood vessel, the specific steps are as follows:

[0027] (1) Collection of vascular material

[0028] Immediately after taking out the heart, wash it with sterile saline, then place it in frozen saline and transport it to the laboratory, then use forceps and scissors to carefully separate the porcine cardiovascular, after removing fat and adherent tissue, clean it with sterile cold saline Thorough flushing of the coronary arteries;

[0029] (2) Preparation of decellularized blood vessels

[0030] Place the isolated coronary artery in sterile distilled water for 24 hours of hypotonic treatment on a shaking table, take it out at the 4th hour and place it in a -80°C refrigerator for 30 minutes, then place it at room temperature for 30 minutes, and repeat the above operation twice; after 24 hours of hypotonic trea...

Embodiment 2

[0039] Embodiment 2, artificial blood vessel is implanted in rat body

[0040]SD rats with a body weight of about 300 g were used as the experimental objects. They were fasted 48 hours before the artificial blood vessel replacement experiment and fed with glucose water instead. Anesthetized by intraperitoneal injection of 0.5ml / 100g of chloral hydrate with a mass fraction of 7%. After the SD rats were completely anesthetized, the hair on the abdomen was removed with a shaver, and the rats were placed in a supine position with their limbs stretched and fixed on the operating table. superior. Disinfect the surgical site with 75% medical alcohol by volume, turn on the shadowless lamp and aim at the abdomen, cut the skin, open the abdominal cavity along the midline of the abdomen, push the intestines and stomach to both sides with wet cotton balls, expose the abdominal aorta, and then use forceps to carefully Isolate the abdominal aortic vessel. Then heparin sodium was injected ...

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Abstract

The invention relates to a preparation method of a composite small-caliber artificial blood vessel, and a product thereof. The preparation method comprises the following steps of decellularizing a coronary artery to obtain a decellularized blood vessel; then heparinizing the decellularized blood vessel, using a mixed electrospinning solution of gelatin and poly L-lactide-caprolactone (PLCL) for spinning, and removing an organic solvent, thus obtaining the small-caliber artificial blood vessel. The prepared artificial blood vessel is a double-layer tissue engineering blood vessel, adopts decellularized porcine coronary artery as an artificial blood vessel inner layer, has good compliance and cytocompatibility, and can promote cell adhesion, proliferation and differentiation, and spreading so as to accomplish reendothelialization of the blood vessel; an outer layer is a blending layer of the PLCL and the gelatin, so that the mechanical property of the blood vessel is improved, cells of smooth muscle and the like are favorably intruded, and a middle layer of the blood vessel is formed; the inner surface of the inner layer can be modified by different drugs such as heparin, so that theantithrombus of the artificial blood vessel is improved, the blood vessel intima formation is promoted, and the blood vessel is favorably rebuilt.

Description

technical field [0001] The invention belongs to the field of medical device preparation, relates to a method for preparing a composite small-diameter artificial blood vessel, and also relates to a product prepared by the method. Background technique [0002] With the increasing number of people suffering from cardiovascular disease (Coronary artery disease, CAD), the demand for artificial blood vessel transplantation is increasing year by year. Clinically, large-diameter blood vessels have been well used, but small-diameter blood vessels (diameter <6 mm) made of the same material are prone to thrombosis, and the reason may be related to the performance of the graft material itself. Autologous blood vessels are considered to be the gold standard for clinical use, but the use of autologous blood vessels will cause great trauma to patients and the number of desirable ones is limited. Therefore, it is particularly important to find blood vessel materials that are similar in p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/22A61L27/26A61L27/36A61L27/50A61L33/10A61F2/06
CPCA61F2/062A61F2210/0076A61F2210/0085A61L27/18A61L27/222A61L27/26A61L27/3625A61L27/3687A61L27/507A61L33/0011A61L33/08C08L5/10C08L89/00C08L67/04
Inventor 胡廷章李文萍冉小琳蔺松王贵学
Owner CHONGQING UNIV
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