Highly efficient phosphate-solubilizing bacteria for soil improvement, and application of highly efficient phosphate-solubilizing bacteria

A phosphate-dissolving bacteria and high-efficiency technology, which is applied in the field of microbial engineering, can solve the problems that phosphorus cannot be absorbed and utilized by plants, the lack of phosphorus utilization, water pollution and soil, etc., to solve the problem of insufficient phosphorus supply in the soil, and the cultivation conditions and methods are simple and easy Line, reduce the effect of chemical fertilizer application

Active Publication Date: 2018-09-14
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, at present, more than 130,000 tons of phosphorus accumulated in the soil in my country cannot be absorbed and utilized by plants.
Long-term application of phosphorus fertilizers will lead to the accumulation of slow-acting phosphorus in the soil, which will cause soil compaction and fertility decline while increasing production and income, and lead to a series of environmental problems such as water pollution and soil pollution.
Therefore, phosphorus deficiency and low utilization rate are important problems to be solved urgently in the production of agriculture in our country.

Method used

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  • Highly efficient phosphate-solubilizing bacteria for soil improvement, and application of highly efficient phosphate-solubilizing bacteria
  • Highly efficient phosphate-solubilizing bacteria for soil improvement, and application of highly efficient phosphate-solubilizing bacteria
  • Highly efficient phosphate-solubilizing bacteria for soil improvement, and application of highly efficient phosphate-solubilizing bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Screening of efficient phosphate-solubilizing bacteria:

[0029] (1) The isolation method of the bacterial strain is a plate screening method: collect activated sludge in a 50mL sterile centrifuge tube and place it in a clean ice box. Weigh 1g of activated sludge, and use 0.9% sterile saline to dilute the sample gradient to make 10 -1 ~10 -5 solution.

[0030] (2) Medium:

[0031] Monkina (PVK) solid medium: calcium phosphate 5.0g, sucrose 10.0g, ammonium sulfate 0.5g, sodium chloride 0.1g, magnesium sulfate heptahydrate 0.1g, potassium chloride 0.2g, manganese sulfate 0.03g, yeast extract 0.5g, 15g agar, 1000mL distilled water, pH 7.0~7.2.

[0032] LB medium: yeast powder 5.0g, peptone 10.0g, sodium chloride 10.0g, distilled water 1000mL, pH 7.0. Add 15g of agar powder to the solid nutrient agar medium.

[0033] (3) Separation and screening of strains: draw 0.1 mL of dilutions of different dilutions, spread evenly on PVK solid medium, place at 30°C for 3 to 5 day...

Embodiment 2

[0035] Identification of highly efficient phosphate-soluble strain YLYP6:

[0036] The basis and method for the detection of morphological characteristics, physiology and biochemistry is the bacterial identification procedure MS(i) / C005-C01. Morphological identification: the bacteria are Gram-negative bacteria; there are spores, middle or apical, and the spores are oval; the bacteria are arranged singly or in pairs. Cultivated on LB solid medium for 24h, the colonies were moist, with smooth and opaque surfaces and irregular edges ( figure 1 ), which is long rod-shaped under the electron microscope, with an average length of 2-3 μm and an average width of 0.2-0.4 μm, see figure 2 .

[0037] Identification of physiological and biochemical characteristics: See Table 1 for the results of physiological and biochemical experiments.

[0038] Table 1 Identification of physiological and biochemical characteristics of YLYP6 bacteria

[0039] Physiological and biochemical t...

Embodiment 3

[0044] Quantitative analysis of phosphorus-solubilizing ability of strain Pseudomonas prosekii YLYP6:

[0045] (1) Preparation of bacterial suspension: use an inoculation loop to pick a small amount of bacteria in 50mL LB liquid medium, incubate at 30°C, 130rpm for 10h, take 40mL of culture solution in a 50mL centrifuge tube, centrifuge at 4°C, 6000rpm for 3min , collect the thalline, wash the thalline 3 times with 0.9% sterile normal saline, and prepare the bacterial density of 10 with normal saline 8 cfu / mL bacterial suspension.

[0046] (2) Phosphate pretreatment: Weigh 20.0g of calcium phosphate, aluminum phosphate or iron phosphate in a large beaker, dissolve it with 2L of ultrapure water ultrasonically, discard the upper solution every 30min, and keep the insoluble phosphate at the bottom of the beaker , repeat ultrasonic washing 2 to 3 times until the upper layer solution is clear, and collect the phosphate precipitated at the bottom of the beaker, put it in a 60°C ove...

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Abstract

The invention discloses highly efficient phosphate-solubilizing bacteria for soil improvement, and application of the highly efficient phosphate-solubilizing bacteria, belonging to the technical fieldof microbiological engineering. The bacterial strain is pseudomonas prosekii YLYP6, is preserved in China Center for Type Culture Collection (CCTCC) of Wuhan University, Wuhan city, China on June 28,2017, and has a preservation number of CCTCC No. M2017390. The bacterial strain supplements the microbial resource library of the phosphate-solubilizing bacteria, is simple in culture conditions andeasy in culture method, and is strong in the phosphate-solubilizing ability; the highly efficient phosphate-solubilizing bacteria can effectively improve the available phosphorus content of the soil in a short time, can maintain lasting phosphate-solubilizing activity in the soil, is high in adaptability, increases the available phosphorus content in the soil, and has strong adaptability, thus having good application potential; the highly efficient phosphate-solubilizing bacteria is suitable for being applied to agricultural production as a microbial agent, and has a broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and in particular relates to a high-efficiency phosphorus-dissolving bacterium for soil improvement and its application. Background technique [0002] Phosphorus is a macronutrient element necessary for plant growth and development. It not only participates in the composition of many important compounds in plants, but also participates in physiological metabolic activities in plants in various ways, determining the yield and quality of crops. In the soil-plant ecosystem has an irreplaceable role. Phosphorus deficiency in soil is one of the main limiting factors in current agricultural production. The available phosphorus in my country's soil accounts for less than 5% of the soil phosphorus content, which is far from meeting the needs of crop growth and development, and must be supplemented by the application of phosphorus fertilizers. Due to the small mobility of phosphate fertili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14C05F11/08C12R1/38
CPCC05F11/08C09K17/14C12N1/20C12N1/205C12R2001/38
Inventor 莫测辉喻乐意冯乃宪李彦文蔡全英李慧
Owner JINAN UNIVERSITY
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