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A kind of osteoinductive collagen-based composite hydrogel and preparation method thereof

A composite hydrogel, osteoinductive technology, applied in pharmaceutical formulations, prostheses, drug delivery, etc., can solve the problems of insufficient osteoinductivity, poor compatibility between the organic phase and particle-bone interface, etc., and achieve excellent tissue induction. , good spatial stability, the effect of improving mechanical properties

Active Publication Date: 2020-06-09
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In summary, the current existing technologies are mostly limited to the simple loading of hydrogel and granular bone, and the interface compatibility between the organic phase and granular bone is poor. Although there are related reports on the use of dopamine for interfacial adhesion, they are mostly limited to the synthesis of high The interaction between molecules and dopamine is obviously insufficient in osteoinductivity, especially in the bionic construction of osteoinductive hydrogel loaded with nano-fishbone and its preparation method based on "collagen chemical modification" and "dopamine self-aggregation assembly". various reports

Method used

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  • A kind of osteoinductive collagen-based composite hydrogel and preparation method thereof
  • A kind of osteoinductive collagen-based composite hydrogel and preparation method thereof
  • A kind of osteoinductive collagen-based composite hydrogel and preparation method thereof

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Embodiment 1

[0031] Use 0.1mol / L, pH 2.0 acetic acid-sodium acetate solution to dissolve pigskin collagen, stir well at 4°C to help dissolve and disperse, obtain a collagen solution with a concentration of 15mg / mL, and then use 0.1mol / L NaOH solution Adjust the pH value of the solution to 7.0, and then incubate at 30°C for 4 hours to obtain a skin collagen hydrogel substrate; soak the prepared skin collagen hydrogel substrate at 20°C 5 wt% of the dry weight of the collagen hydrogel base material) was reacted in a chemical modifier for 8 hours. Subsequent addition of dopamine and H 2 o 2 , the dosage of dopamine is 5wt% of skin collagen hydrogel base material dry weight, H 2 o 2 The dosage is 0.5% by weight of dopamine, and the reaction is 3 hours. Then add nano-tilapia bones (the amount is 5wt% of the dry weight of the skin collagen hydrogel substrate), adjust the loading temperature to 30° C., and the pH to 7.0. After reacting for 4 hours, use ultrapure water to fully wash 3 times, an...

Embodiment 2

[0035] Use 0.1mol / L acetic acid-sodium acetate solution with a pH of 2.0 to dissolve sheepskin collagen, stir well at 10°C to help dissolve and disperse, and obtain a collagen solution with a concentration of 10mg / mL, and then use 0.1mol / L NaOH solution to adjust The pH value of the solution was brought to 7.5, and then incubated at a constant temperature at 37°C for 8h to obtain a skin collagen hydrogel substrate; the prepared skin collagen hydrogel substrate was immersed at 30°C in a solution containing oxidized hyaluronic acid (amount of 6wt% of the dry weight of the hydrogel base material) was reacted in the chemical modifier for 8 hours. Subsequent addition of dopamine and H 2 o 2 , the amount of dopamine is 10wt% of dry weight of skin collagen hydrogel substrate, H 2 o 2 The dosage is 1.0wt% of dopamine weight, and the reaction is 3h. Then add nano-salmon fish bones (the amount is 10wt% of the dry weight of the skin collagen hydrogel substrate), adjust the loading te...

Embodiment 3

[0037] Use 0.15mol / L, pH 2.5 acetic acid-sodium acetate solution to dissolve bovine skin collagen, stir well at 4°C to help dissolve and disperse, obtain a collagen solution with a concentration of 5mg / mL, and then use 0.1mol / L NaOH solution to adjust The pH value of the solution was brought to 8.0, and then incubated at a constant temperature at 37°C for 6h to obtain a skin collagen hydrogel substrate; the prepared skin collagen hydrogel substrate was immersed at 30°C 5wt% of the dry weight of the hydrogel base material) was reacted in the chemical modifier for 8h. Subsequent addition of dopamine and H 2 o 2 , the amount of dopamine is 6wt% of the dry weight of the skin collagen hydrogel substrate, H 2 o 2 The dosage is 2.0wt% by weight of dopamine, and the reaction takes 2 hours. Then add nano-grass carp fish bones (the amount is 8wt% of the dry weight of the skin collagen hydrogel substrate), adjust the load temperature to 30 ° C, and pH to 6.5. After reacting for 12 ho...

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Abstract

The invention discloses an osteoinductive collagen based composite hydrogel and a preparation method thereof. The preparation method comprises following steps: preparing a leather collagen solution byadopting an acidic buffer solution, fully stirring to promote the dissolution and dispersion, slowly adjusting the pH of the solution to 6.0-8.0 by using an alkali solution, then placing the solutionin a thermostat incubator, carrying out incubation to obtain a leather collagen hydrogel basic material; soaking the leather collagen hydrogel basic material in a chemical modifying agent solution, adding dopamine and an oxidizing agent, fully carrying out reactions to obtain a dopamine modified leather collagen hydrogel basic material; adding nano fish bones, adjusting the temperature and pH, after reactions, fully washing the reaction product by ultrapure water, and then sterilizing the reaction product by irradiation to obtain the osteoinductive collagen based composite hydrogel. The hydrogel has the advantage that the compatibility of interface between granular bones in the prepared hydrogel and organic phase hydrogel is greatly improved, thus the prepared hydrogel has an excellent osteoinductive property, and the additional value of leather collagen and fish bones is increased.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to an osteoinductive collagen-based composite hydrogel and a preparation method thereof. Background technique [0002] The repair of bone defects caused by trauma, tumor, inflammation and surgery has always been a major problem faced by orthopedic surgeons and material scientists. In today's clinical treatment, the large bone structure in the transplanted bone delays the material exchange and signal transduction between materials and cells in bone repair. The design and development of granular bone repair materials came into being. The main component of natural bone is collagen Fiber and hydroxyapatite nanocrystals, due to the limited material of autologous bone tissue, granular bone is mainly derived from animal bone tissue (bovine bone, pig bone, coral bone, fish bone, etc.) or hydroxyapatite, silicic acid with similar composition Calcium, calcium phosphate, calcium carbonat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/52A61L27/36A61L27/24A61L27/18A61L27/50
CPCA61L27/18A61L27/24A61L27/3608A61L27/50A61L27/52A61L2400/12A61L2430/02C08L79/02
Inventor 胡杨余小月朱士臣吴潇扬赵思明熊善柏
Owner HUAZHONG AGRI UNIV
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