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Uric acid-lowering marine fish oligopeptide and preparation method thereof

A marine fish oligopeptide and uric acid-lowering technology, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of general desalination effect, high cost, high energy consumption, etc., so as to relieve muscle pain and salinity. Less and more economical effect

Active Publication Date: 2018-09-21
艾苛密(上海)健康科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Usually the salt content of marine fish meal is 2-5%. Adjusting the pH value during the enzymatic hydrolysis process will bring in some salt. Most of these salts are enriched into the final peptide powder, making the salt content of peptide powder as high as 10 25%, while the effect of desalination purely by membrane separation is mediocre, and the energy consumption is high and the cost is high

Method used

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  • Uric acid-lowering marine fish oligopeptide and preparation method thereof

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preparation example Construction

[0046] The preparation method of the enzyme activator is as follows: mix magnesium acetate and dimethyl carbonate at a mass ratio of 3:0.1, and stir at 500 r / min for 30 minutes at 35°C to obtain the enzyme activator.

[0047] Glucose, CAS number: 50-99-7, was purchased from Shandong Zibo Yufengyuan Sugar Co., Ltd.

[0048] Activated carbon powder, particle size 40 mesh, food grade, was purchased from Gongyi City Songshan Filter Material Activated Carbon Factory.

[0049] Aspergillus niger, model NY-3033, with an effective viable count of 15 billion CFU / g, was purchased from Jining Yuyuan Biotechnology Co., Ltd.

[0050] Yeast, with an effective viable count of 20 billion CFU / g, was purchased from Guangzhou Weiyuan Biotechnology Co., Ltd.

[0051] Bacillus subtilis, with an effective viable count of 10 billion CFU / g, was purchased from Cangzhou Fangyuan Bioengineering Co., Ltd.

[0052] 5000Da ultrafiltration membrane, made of PES, model MWCO500, purchased from General Electr...

Embodiment 1

[0060] A method for preparing uric acid-reducing marine fish oligopeptides, comprising the following steps:

[0061] Ⅰ. Remove the skin and bones of the marine fish and take the fish meat, wash the fish meat with 2°C cold water for 3 times, the mass ratio of cold water to fish meat for each washing is 2:1, let it stand for 4 minutes after each washing, and drain the water, Get pre-treated fish meat;

[0062] Ⅱ. Grinding the above-mentioned pretreated fish with a meat grinder, the production capacity of the meat grinder is 120kg / h, and the power is 0.55kw to obtain minced fish with a diameter of 8mm, according to the mass-volume ratio of 1:4 (g / mL) Add water to the obtained minced fish meat, place it in a homogenizer at 30°C and 1500r / min and stir for 30min to obtain a homogenate;

[0063] Ⅲ. The above-mentioned homogenate was adjusted to pH 7 with 0.5mol / L sodium hydroxide solution, and flavor protease and enzyme activator were added. The mass ratio of the homogenate, flavor...

Embodiment 2

[0067] A method for preparing uric acid-reducing marine fish oligopeptides, comprising the following steps:

[0068] Ⅰ. Remove the skin and bones of the marine fish and take the fish meat, wash the fish meat with 2°C cold water for 3 times, the mass ratio of cold water to fish meat for each washing is 2:1, let it stand for 4 minutes after each washing, and drain the water, Get pre-treated fish meat;

[0069] Ⅱ. Grinding the above-mentioned pretreated fish with a meat grinder, the production capacity of the meat grinder is 120kg / h, and the power is 0.55kw to obtain minced fish with a diameter of 8mm, according to the mass-volume ratio of 1:4 (g / mL) Add water to the obtained minced fish meat, place it in a homogenizer at 30°C and 1500r / min and stir for 30min to obtain a homogenate;

[0070] Ⅲ. The above-mentioned homogenate was adjusted to pH 7 with 0.5mol / L sodium hydroxide solution, and flavor protease and enzyme activator were added. The mass ratio of the homogenate, flavor...

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Abstract

The invention relates to uric acid-lowering marine fish oligopeptide and a preparation method thereof. According to the preparation method, marine fish is taken as a raw material, the fish meat of themarine fish is treated by using methods such as a steam explosion treatment method, a protease enzymolysis method and a microbial fermentation method, and desalting treatment is carried out by combining ultrasonic waves with a membrane separation technique, so that the uric acid-lowering marine fish oligopeptide product can be finally obtained; the obtained acid-lowering marine fish oligopeptideproduct is stable in properties, high in contents of active components and low in salt content, has effects of reducing uric acid, improving endurance, relieving muscle pain and the like, can be matched with euglena for use, is also used as a food additive, a raw material for health care products and the like, has a high economic value, and is suitable for large-scale industrialized production.

Description

technical field [0001] The invention relates to the field of marine biological products and biotechnology processing, in particular to a uric acid-reducing marine fish oligopeptide and a preparation method thereof. Background technique [0002] With the changes in modern lifestyles including high-fat, high-purine diets and fructified beverages, the proportion of people with elevated blood uric acid levels in physical examinations is increasing. Epidemiological studies have shown that changes in uric acid levels and the prevalence of hyperuricemia in the past 100 years have similar epidemic trends to hypertension, obesity, diabetes, and kidney disease. The prevalence of hyperuricemia in my country has increased by about 10 times on average in the past 10 years. As a disease involving multiple systems, hyperuricemia can directly lead to gout, hyperuricemia nephropathy, aggravate atherosclerosis, islet resistance, etc. The harm cannot be ignored. Scientific research has found...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34
CPCC07K1/14C07K1/34C12P21/06
Inventor 张露引
Owner 艾苛密(上海)健康科技股份有限公司
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