A kind of phenolic acid grafted chitosan oligosaccharide, preparation method, and application of modified water-based polyurethane
A technology of water-based polyurethane and chitosan oligosaccharides, which is applied in the field of phenolic acid grafted chitosan oligosaccharides and its modified water-based polyurethanes, and its preparation method, which can solve the problems of poor solubility and achieve the effect of improving biodegradability and good application prospects
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Embodiment 1
[0023] Step 1: Preparation of gallic acid grafted chitosan oligosaccharide (GA-OCS)
[0024] Dissolve 1g of chitosan oligosaccharide in citric acid buffer solution (100mL, pH 6.5), add 1.7g of gallic acid while shaking at 30°C, slowly add laccase (153U) after the dissolution is complete, and bubbling reaction with oxygen After 24 hours of reaction, the water-insoluble substances were filtered off, and the filtrate was concentrated at 50°C by a rotary evaporator, then dialyzed in deionized water for 24 hours, and phenolic acid-chitooligosaccharide grafted products were obtained after freeze-drying;
[0025] Step 2: Add polytetrahydrofuran ether diol (20g) and isophorone diisocyanate (8.89g) after vacuum dehydration in a dry three-necked flask, stir well, add 2 drops of catalyst dibutyltin dilaurate, in React at 70°C for 1.5h; adjust the temperature to 80°C, add 1.45g of dimethylolpropionic acid, and continue to react for 2.5h to obtain a waterborne polyurethane prepolymer;
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Embodiment 2
[0028] Step 1: Preparation of protocatechuic acid grafted chitosan oligosaccharide (PA-OCS)
[0029] Dissolve 1g of chitosan oligosaccharide in citric acid buffer solution (100mL, pH 6.5), add 1.38g of protocatechuic acid during constant shaking at 30°C, slowly add laccase (153U) after the dissolution is complete, and ventilate with oxygen Bubble reaction for 24 hours, after the reaction, filter out water-insoluble substances, use a rotary evaporator to concentrate the filtrate at 50 ° C, place it in deionized water for dialysis for 24 hours, and obtain the phenolic acid-chitooligosaccharide grafted product after freeze-drying;
[0030] Step 2: Add polytetrahydrofuran ether diol (20g) and isophorone diisocyanate (8.89g) after vacuum dehydration in a dry three-necked flask, stir well, add 2 drops of catalyst dibutyltin dilaurate, in React at 70°C for 1.5h; adjust the temperature to 80°C, add 1.45g of dimethylolpropionic acid, and continue to react for 2.5h to obtain a waterborn...
Embodiment 3
[0033] Step 1: Preparation of para-hydroxybenzoic acid grafted chitosan oligosaccharide (HA-OCS)
[0034] Dissolve 1g of chitosan oligosaccharide in citric acid buffer solution (100mL, pH 6.5), add 1.54g of p-hydroxybenzoic acid during constant shaking at 30°C, slowly add laccase (153U) after the dissolution is complete, and ventilate with oxygen Bubble reaction for 24 hours, after the reaction, filter out water-insoluble substances, use a rotary evaporator to concentrate the filtrate at 50 ° C, place it in deionized water for dialysis for 24 hours, and obtain the phenolic acid-chitooligosaccharide grafted product after freeze-drying;
[0035] Step 2: Add polytetrahydrofuran ether diol (20g) and isophorone diisocyanate (8.89g) after vacuum dehydration in a dry three-necked flask, stir well, add 2 drops of catalyst dibutyltin dilaurate, in React at 70°C for 1.5h; adjust the temperature to 80°C, add 1.45g of dimethylolpropionic acid, and continue to react for 2.5h to obtain a wa...
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