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A kind of fermentation production process of Paecilomyces cs-4

A Paecilomyces bat moth, production process technology, applied in the field of microorganisms, can solve the problems of low active ingredient content of fermentation products, low fermentation yield, long fermentation cycle, etc., to achieve shortened fermentation process cycle, high nutritional value, nucleoside The effect of content increase

Active Publication Date: 2021-02-19
JIANGXI GUOYAO PHARMA LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Fermentation and cultivation of Cordyceps strains is the prerequisite for the production of Cordyceps products. The traditional fermentation process of Cordyceps strains has problems such as low fermentation yield, long fermentation time and high cost.
Therefore, processes such as static fermentation, batch fermentation, and fed-batch fermentation have emerged, but these fermentation processes still have various problems, such as high bacterial contamination rate, long fermentation cycle, high cost, and low content of active ingredients in the fermentation product Wait

Method used

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  • A kind of fermentation production process of Paecilomyces cs-4

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preparation example Construction

[0030] The preparation method of the slant and shake flask medium is as follows: weigh the bran that has passed through a 40-mesh sieve according to the calculated amount, calculate the amount of water added by adding 6g of bran per 100mL of water, heat to boiling, maintain boiling for 30 minutes, and filter to obtain the filtrate. Then add other components, heat and / or add water to dissolve, add water to make up volume, pack and sterilize. The preparation method of the culture medium of the seed tank, the breeding tank and the fermenting tank is as follows: measure 50% of water, add various culture medium components therein, heat and dissolve, add water to constant volume, and then sterilize.

[0031]The sterilization methods of various media are high-pressure steam sterilization, the steam pressure is 0.09-0.12Mpa, the temperature is 121±1°C, and the sterilization time is 30-40 minutes.

Embodiment 1

[0035] The composition of the slant medium is: glucose 2%, peptone 0.2%, bran 0.4%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.03%, histidine 0.1%, agar 2%, water balance, pH natural. Take 1cm with an inoculation spatula 2 The Cs-4 strain preserved on the left and right sides was evenly spread on the inclined surface, and cultured at a temperature of 15° C. and a humidity of 85% for 7 days. When the growth is mature, the appearance inspection is carried out, and the culture characteristics: the spores and hyphae are plump, and they are white or light yellow plush. Remove the slant with poor appearance, and store the slant with good growth in the refrigerator at 1-8°C for later use.

[0036] The composition of shake flask medium is: glucose 1%, peptone 0.2%, bran 0.4%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.03%, zinc sulfate 0.03%, histidine 0.1% and vitamin B 1 0.05%, water balance, pH natural. Under sterile conditions, take 1cm with an inocu...

Embodiment 2

[0042] The difference between embodiment 2 and embodiment 1 is that the medium of the seed tank contains 0.4% of the powder of notoginseng, the medium of the breeding tank contains 0.5% of the powder of notoginseng, and the medium of the fermenter contains 0.5% of the powder of notoginseng. According to the same process conditions as in Example 1, the Cs-4 strain was fermented and cultivated to finally obtain the Cs-4 Cordyceps fungus powder. Compared with the Cordyceps fungus powder of Example 1, the content of ergosterol in the fungus powder prepared in this embodiment is significantly improved, and the fungus powder contains saponin.

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Abstract

The present invention relates to a fermentation production process of Paecilomyces hematalis Cs-4, comprising inclined plane culture, shake flask culture, seed tank culture, breeding tank culture and fermenter tank culture, and the fermented liquid after fermentation can be used as shake flask culture seed. Histidine in slant medium, histidine and vitamin B in shake flask medium 1 , the seed tank medium contains purine, the propagation tank medium contains purine and pyrimidine, the fermenter medium contains purine and pyrimidine, and the cultured bacteria powder has high nucleoside content. After adding Panax notoginseng powder to the liquid fermentation medium, the obtained Cordyceps fungus powder contains saponin, and the content of ergosterol also increases. The use of red light irradiation during shake flask culture can increase the content of active ingredients in the bacterial powder. The process of the invention has the advantages of simple operation, short fermentation period and low cost, and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a fermentation production process of Paecilomyces hematalis Cs-4. Background technique [0002] Cordyceps sinensis is only one of more than 190 kinds of Chinese caterpillar fungus, mainly produced on the Qinghai-Tibet Plateau, also referred to as "Cordyceps". Cordyceps sinensis is a complex of the subseapotta and larva corpses of the ergot fungus Cordyceps sinensis that parasitizes on the larvae of bat moths. Famous and precious medicinal food tonic, it is flat in nature and sweet in taste, and has the functions of invigorating the lungs and kidneys, relieving cough, benefiting deficiency, and nourishing essence. According to clinical research reports, Cordyceps sinensis has the effects of nourishing lung yin, tonifying kidney yang, relieving cough and reducing phlegm, anti-cancer and anti-aging. Cordyceps sinensis can be used for pulmonary tuberculosis, hemoptysis, impotence an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/79
CPCC12N1/14C12M21/16
Inventor 吴彪万义斌葛友群左飞鸿杨明何琳李进进
Owner JIANGXI GUOYAO PHARMA LLC
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