Diethylstilbestrol-based antibiotic immunoaffinity column, preparation method and uses thereof
A technology of diethylstilbestrol and immunoaffinity, which is applied in the field of diethylstilbestrol antibiotic immunoaffinity column and preparation thereof, can solve the problems of strong specialization of instruments, high technical requirements and high cost of measuring samples, and achieves easy operation, high purification efficiency, and guaranteed antigen The effect of binding capacity
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Embodiment 1
[0022] The main structure of the diethylstilbestrol antibiotic immunoaffinity column involved in this embodiment includes a column body 1, a cover plate 2, an injection port 3, a first sieve plate 4, a filler 5, a second sieve plate 6 and a sample outlet 7; The top of the cylinder 1 with a tubular structure is provided with a cover plate 2 with a circular sheet structure, and the center of the cover plate 2 is provided with a sample inlet 3 with a circular tubular structure. The inner upper space of the cylinder 1 is a cavity, and the cylinder 1 The first sieve plate 4 with a circular sheet structure with a sieve hole is arranged in the middle of the interior of the cylinder, the inner lower space of the column 1 is filled with fillers 5, and the bottom of the column 1 is provided with a circular sheet with a sieve hole. The structure of the second sieve plate 6, the second sieve plate 6 is provided with a sample outlet 7 of a conical tubular structure with a wide top and a nar...
Embodiment 2
[0024] The specific process of the preparation method of the diethylstilbestrol antibiotic immunoaffinity column involved in this example includes five steps of activation, coupling, connection, cross-linking and column packing:
[0025] (1) Activation: Fully rinse the pre-swelled agarose gel sepharose2B with a concentration of 2% with distilled water, wash away the remaining ethanol in the agarose gel sepharose2B, the volume ratio of distilled water to the agarose gel sepharose2B is 20:1, use Funnel filters liquid, obtains wet gel, takes by weighing 5 grams of wet gel and 7.5 milliliters of molar masses and is the NaOH (sodium hydroxide) aqueous solution of 0.8M, 2 milliliters of mass percentage concentrations and is 30% epichlorohydrin aqueous solution and 5 mL of NaBH at a concentration of 2 mg / mL 4 (Sodium borohydride) aqueous solution is carried out shaking table reaction under the condition of 25 ℃ for 8 hours at a temperature, after the reaction finishes, wash the wet g...
Embodiment 3
[0031] The specific technological process of the diethylstilbestrol antibiotic immunoaffinity column purification and the enrichment chicken in the diethylstilbestrol antibiotic that this embodiment involves is: first, add 10 milliliters of methyl alcohols in No. 50 milliliter centrifuge tubes with 5.0 g homogeneous chicken The aqueous solution was vortexed for 2 minutes, ultrasonically extracted for 5 minutes, centrifuged at 6000r / min for 5 minutes, the supernatant was transferred to No. 2 50ml centrifuge tube with stopper, and then 10 Milliliter methanol water solution, vortexed for 2min, then ultrasonically extracted for 5min, again transferred the supernatant to No. 2 50ml stoppered centrifuge tube, dilute the supernatant to 25ml with methanol water solution, and pipetted 5ml supernatant Add 20 ml of PBS to the No. 3 50 ml centrifuge tube to dilute the supernatant; secondly, take out the diethylstilbestrol immunoaffinity column after equilibrating at room temperature for 30...
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