On-machine serum diluent for chemiluminescence instrument and preparation method of on-machine serum diluent

A chemiluminescence and diluent technology, applied in the field of immunological reagents, can solve the problems of low detection results, hospital loss, patient injury, etc., and achieve the effect of reducing non-specific adsorption

Inactive Publication Date: 2018-11-27
SUZHOU YOUJUN ENVIRONMENTAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the specific combination of antigen and antibody, it was first used in radioimmunoassay technology, and then enzyme immunoassay technology was derived because of its pollution problem. Although enzyme immunology technology solved the pollution problem well, its sensitivity could not reach that of radioimmunoassay. Height, and then the birth of chemiluminescent immune technology has solved these two problems
However, when the specific binding reaction between the antigen and t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] An on-board serum diluent for a chemiluminescence instrument, which is composed of the following components in parts by weight: 33 parts of dipotassium hydrogen phosphate, 39 parts of potassium dihydrogen phosphate, 16 parts of sodium bicarbonate, 15 parts of zinc sulfate, heparin 26 parts of sodium, 11 parts of sorbitol, 7 parts of glycine, 5 parts of polyethylene oxide, 6 parts of polyethylene glycol, 2 parts of nonylphenol polyoxyethylene (10) ether, 0.2 parts of sodium caseinate, 0.5 parts of gentamicin parts, 0.3 parts of sodium azide, and 120 parts of deionized water.

[0016] A method for preparing a chemiluminescence instrument on-board serum diluent, comprising the following steps:

[0017] Step 1: Add sodium caseinate into deionized water, stir at 5°C at a stirring speed of 50 rpm for 70 hours, then add sodium azide, and continue stirring for 20 minutes to obtain solution A;

[0018] Step 2. Add dipotassium hydrogen phosphate, potassium dihydrogen phosphate, ...

Embodiment 2

[0021] An on-board serum diluent for a chemiluminescent instrument, which is composed of the following components in parts by weight: 38 parts of dipotassium hydrogen phosphate, 33 parts of potassium dihydrogen phosphate, 16 parts of sodium bicarbonate, 16 parts of zinc sulfate, heparin 19 parts of sodium, 12 parts of sorbitol, 8 parts of glycine, 4 parts of polyethylene oxide, 6 parts of polyethylene glycol, 1 part of nonylphenol polyoxyethylene (10) ether, 0.2 parts of sodium caseinate, 0.4 parts of gentamicin parts, 0.2 parts of sodium azide, and 130 parts of deionized water.

[0022] A method for preparing a chemiluminescence instrument on-board serum diluent, comprising the following steps:

[0023] Step 1: Add casein sodium into deionized water, stir at 4°C for 68 hours at a stirring speed of 50 rpm, then add sodium azide, and continue stirring for 15 minutes to obtain solution A;

[0024] Step 2: Add dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodiu...

Embodiment 3

[0027] An on-board serum diluent for a chemiluminescent instrument, which is composed of the following components in parts by weight: 29 parts of dipotassium hydrogen phosphate, 29 parts of potassium dihydrogen phosphate, 16 parts of sodium bicarbonate, 13 parts of zinc sulfate, heparin 19 parts of sodium, 9 parts of sorbitol, 8 parts of glycine, 5 parts of polyethylene oxide, 6 parts of polyethylene glycol, 2 parts of nonylphenol polyoxyethylene (10) ether, 0.2 parts of sodium caseinate, 0.5 parts of gentamicin parts, 0.1 parts of sodium azide, and 100 parts of deionized water.

[0028] A method for preparing a chemiluminescence instrument on-board serum diluent, comprising the following steps:

[0029] Step 1: Add sodium caseinate into deionized water, stir at 6°C for 60 hours at a stirring speed of 50 rpm, then add sodium azide, and continue stirring for 20 minutes to obtain solution A;

[0030] Step 2. Add dipotassium hydrogen phosphate, potassium dihydrogen phosphate, so...

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PUM

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Abstract

The invention discloses an on-machine serum diluent for a chemiluminescence instrument and a preparation method of the on-machine serum diluent. The on-machine serum diluent contains the following components in parts by weight: 21-45 parts of dipotassium phosphate, 22-50 parts of potassium dihydrogen phosphate, 11-22 parts of sodium hydrogen carbonate, 11-19 parts of zinc sulfate, 15-31 parts of heparin sodium, 7-17 parts of sorbitol, 3-11 parts of glycine, 3-7 parts of polyoxyethylene, 4-9 parts of polyethylene glycol, 1-4 parts of nonylphenol polyoxyethylene (10) ether, 0.1-0.4 part of casein sodium, 0.2-0.6 part of gentamicin, 0.1-0.4 part of sodium azide and 80-180 parts of deionized water. The on-machine serum diluent for the chemiluminescence instrument is prepared by virtue of the synergistic effect among the components, can be used for effectively diluting a serum sample and can be rapidly and uniformly mixed with serum, a detection result is not interfered, and the nonspecificadsorption can be reduced.

Description

technical field [0001] The invention relates to the technical field of immune reagents, in particular to an on-board serum diluent for a chemiluminescent instrument and a preparation method thereof. Background technique [0002] With the rapid development of biology, the cognition of the human body is becoming more and more in-depth and refined, and the detection of biological macromolecules such as proteins and accounting is increasing, and its technical means are constantly innovating and improving. Among them, immunology is a particularly prominent branch of modern medical biological research, which is based on the specific combination of antigens and antibodies. Based on the specific combination of antigen and antibody, it was first used in radioimmunoassay technology, and then enzyme immunoassay technology was derived because of its pollution problem. Although enzyme immunology technology solved the pollution problem well, its sensitivity could not reach that of radioim...

Claims

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Application Information

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IPC IPC(8): G01N1/38
CPCG01N1/38
Inventor 凌云凌天阳金可心夏戊君
Owner SUZHOU YOUJUN ENVIRONMENTAL SCI & TECH
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