Wheat-based protein polypeptide and modified starch compounded wheat grain simulant and preparation method thereof
A wheat starch and simulant technology, which is applied in the fields of food supplementary additives and food processing, can solve the problems of unfavorable storage of liquid products, unsuitable for large-scale production, incomplete nutritional structure, etc., so as to improve the immunity of the body and facilitate the utilization of the body. , the effect of easy operation
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preparation example 1
[0070] Preparation of Wheat Germ Peptide Extract
[0071] Put 1.5 kg of dried defatted wheat germ into a superfine pulverizer and pass through a 200-mesh sieve. Add distilled water to dissolve the obtained wheat germ powder at a ratio of 1:10 (kg / L), adjust the pH to 9, and store it at 50°C. Stir under low temperature for 2 hours to fully dissolve the alkali extraction twice, heat to 80°C after alkali extraction, add high-temperature-resistant α-amylase, the dosage of α-amylase is 50U / g germ powder, and enzymolyze at 80°C for 1 hour; filter or centrifuge, Retain the liquid to obtain 16.5 kg of enzymolyzed germ pulp; heat the obtained germ pulp in an autoclave at 121°C for 15 minutes to deactivate the α-amylase enzyme, thereby obtaining the α-amylase enzymatically hydrolyzed germ liquid; then adjust to pH 5, let stand to separate layers, discard the supernatant to take the bottom sediment, dissolve in distilled water, adjust the pH of the germ solution to 8.0, add trypsin, the ...
preparation example 2
[0073] Preparation of wheat germ globulin
[0074] Add 100g of 200 mesh defatted wheat germ powder into 1L of distilled water, stir and extract at 25°C for 2 hours, then use a power of 200W for ultrasonic treatment for 10 minutes; then centrifuge at 5000r / min for 20 minutes at low temperature, discard the supernatant, and add 800ml of water to the precipitate for two times Extract, centrifuge, then add 800mL 0.5M NaCl solution to the precipitate, stir and extract at 25°C for 1h, centrifuge at 5000r / min for 30min at low temperature, collect the supernatant, then add 600mL 0.5M NaCl solution to the precipitate to extract again, collect and combine Supernatant, the obtained supernatant was adjusted to pH 4.0 with 1M HCl, centrifuged at 6000r / min at low temperature for 20min, discarded the supernatant to take the precipitate, dispersed and washed the precipitate with distilled water, centrifuged again at 6000r / min, and frozen the obtained precipitate Dry to obtain about 16.6 g of ...
preparation example 3
[0076] Preparation of wheat malt syrup
[0077] Add pullulanase, β-amylase and maltase in a mass ratio of 3:4:6 to 30wt% wheat starch solution, wherein the concentration of maltase is 300IU / mL, and stir the enzymatic reaction at 75°C for 2h to obtain Enzyme liquid, heat the enzymatic liquid for 10 minutes to inactivate the enzyme, distill and concentrate to obtain a concentrated liquid, filter the concentrated liquid with a 5 μm microporous membrane to obtain maltose syrup, adjust to the required concentration with distilled water, pasteurize, and cool.
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