Method for cultivating shiitake mushrooms with liquid spawns

A technology for liquid strains and shiitake mushrooms, which is applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems of restricting shiitake mushrooms, complicated fruiting process, weak resistance of shiitake mushroom mycelium, etc., shortening the fruiting time and shortening the cultivation period. , the effect of short seed production time

Inactive Publication Date: 2018-12-11
铜陵盛牛菌业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the mycelial resistance of shiitake mushrooms is weaker than that of oyster mushrooms, the cultivation and fruiting process is much more complicated than that of oyster mushrooms, which limits the application of liquid strains to cultivation of shiitake mushrooms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The method for cultivating shiitake mushrooms with liquid strains provided by the present invention includes the following steps:

[0024] (1) Preparation of liquid culture medium: add fructose, sucrose, wheat bran, soy flour, corn flour, peptone, yeast extract, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, agar, soybean oil, and mulberry leaves to the fermentation tank The extract and water are stirred and dissolved to obtain a liquid culture medium;

[0025] The mass percentages of the raw materials in the liquid culture medium are: fructose 0.7%, sucrose 1.6%, wheat bran 2.8%, soybean meal 1%, corn flour 2.5%, peptone 1.8%, yeast extract 1.7%, potassium dihydrogen phosphate 0.2% %, magnesium sulfate 0.05%, zinc sulfate 0.01%, agar 0.04%, soybean oil 0.2%, Broussonetia papyrifera extract 0.05%, water balance; among them, Broussonetia papyrifera extract is crushed to 10 meshes and 80% ethanol is added Reflux extraction for 1h, filter, recover ethanol fro...

Embodiment 2

[0033] The method for cultivating shiitake mushrooms with liquid strains provided by the present invention includes the following steps:

[0034] (1) Preparation of liquid culture medium: add fructose, sucrose, wheat bran, soy flour, corn flour, peptone, yeast extract, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, agar, soybean oil, and mulberry leaves to the fermentation tank The extract and water are stirred and dissolved to obtain a liquid culture medium;

[0035] The mass percentages of the raw materials in the liquid culture medium are: fructose 1.1%, sucrose 1.1%, wheat bran 3.5%, soybean meal 1.5%, corn meal 2%, peptone 2.3%, yeast extract 1.3%, potassium dihydrogen phosphate 0.3% %, Magnesium sulfate 0.15%, Zinc sulfate 0.04%, Agar 0.08%, Soybean oil 0.05%, Broussonetia papyrifera extract 0.3%, water balance; Among them, Broussonetia papyrifera extract is pulverized to 40 meshes and 90% ethanol is added. Reflux extraction for 3h, filter, recover ethanol ...

Embodiment 3

[0043] The method for cultivating shiitake mushrooms with liquid strains provided by the present invention includes the following steps:

[0044] (1) Preparation of liquid culture medium: add fructose, sucrose, wheat bran, soy flour, corn flour, peptone, yeast extract, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, agar, soybean oil, and mulberry leaves to the fermentation tank The extract and water are stirred and dissolved to obtain a liquid culture medium;

[0045] The mass percentages of the raw materials in the liquid culture medium are: fructose 1%, sucrose 1.4%, wheat bran 3.2%, soybean meal 1.2%, corn flour 2.2%, peptone 2%, yeast extract 1.5%, potassium dihydrogen phosphate 0.25 %, Magnesium sulfate 0.12%, Zinc sulfate 0.02%, Agar 0.05%, Soybean oil 0.1%, Broussonetia papyrifera extract 0.15%, water balance; Among them, Broussonetia papyrifera extract is pulverized to 20 meshes and 85% ethanol is added Reflux extraction for 2h, filter, recover ethanol fr...

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PUM

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Abstract

The invention discloses a method for cultivating shiitake mushrooms with liquid spawns, relating to the technical field of edible fungi cultivation. The method comprises the following steps: preparation of a liquid spawn culture medium, liquid spawn cultivation, preparation of a culture medium, bagging inoculation, hypha culture, and mushroom fruiting period management. Rational optimization of the liquid spawn culture medium and the culture medium as well as daily management and control of cultivation are achieved. Therefore, high activity of liquid spawns for shiitake mushrooms is achieved.The shiitake mushrooms are resistant to diseases. Mycelial biomass and polysaccharide yield are high. Through inoculation and cultivation, mushroom hyphae grow quickly and are dense and white. Sporocarps are robust. The mushroom fruiting period is shortened. High biotransformation efficiency and high yield are obtained. Therefore, liquid spawns are applied to cultivation of shiitake mushrooms successfully. Additionally, the cultivation period is greatly shortened. The natural bagging days are only 40-42. For 94-96 days, shiitake mushrooms grow mature.

Description

Technical field [0001] The invention relates to the technical field of edible fungi cultivation, in particular to a method for cultivating shiitake mushrooms with liquid strains. Background technique [0002] Shiitake mushrooms, known as "mountain treasure" in the folk, are a kind of cultivation fungus for food and medicine. Traditional Chinese medicine theory believes that shiitake mushrooms are sweet, calm and cool in nature, enter the liver and kidney meridians, and have the functions of nourishing the liver and kidney, invigorating the spleen and stomach, nourishing the mind, soothing the nerves, and beautifying the skin, and the lentinan, unsaturated fatty acids, and double disaccharides contained in the shiitake mushrooms Sulfur compounds, ergosterol and other nutrients have the effects of improving immunity, anti-virus, anti-cancer treatment, and protecting liver. Shiitake mushrooms have high nutritional and health value. [0003] The cultivation of shiitake mushrooms in my...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20A01G18/40
CPCA01G18/00A01G18/20A01G18/40
Inventor 方家仓
Owner 铜陵盛牛菌业有限责任公司
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