Oral attenuated lyophilized vaccine for swine fever and preparation method thereof

A swine fever attenuating virus and swine fever mouth technology, applied in biochemical equipment and methods, vaccines, freeze-dried transportation, etc., can solve problems such as unfavorable growth of pigs, and achieve high antibody titers, convenient administration, and good immune effects. Effect

Inactive Publication Date: 2018-12-11
张志刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the CSF vaccines used in my country mainly include injection-type vaccines such as the attenuated strain of CSF, rabbit spleen lymphoid tissue vaccine, and suckling pig kidney cell vaccine. They are mainly injected subcutaneously or intramuscularly. Only holding in place will cause the pigs to stress, which is not good for the growth of the pigs

Method used

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  • Oral attenuated lyophilized vaccine for swine fever and preparation method thereof
  • Oral attenuated lyophilized vaccine for swine fever and preparation method thereof
  • Oral attenuated lyophilized vaccine for swine fever and preparation method thereof

Examples

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Embodiment 1

[0034] Embodiment 1: a kind of oral attenuated lyophilized vaccine for classical swine fever, comprising attenuated classical swine fever virus, lyoprotectant and vaccine diluent, is characterized in that, comprises mucosa immune adjuvant and immunopotentiator in the described vaccine diluent, so The mucosal immune adjuvant includes propolis extract, levamisole and carbomer, and the immune enhancer includes astragalus polysaccharide and polygonatum polysaccharide.

[0035] The lyoprotectant includes 3% bovine serum albumin BSA, 2% mannitol, 2% glycine, 5% lactose and 88% deionized water in mass percent.

[0036] Each liter of the vaccine diluent contains: 130mM sodium chloride, 3.0mM potassium chloride, 10mM disodium hydrogen phosphate, 2mM sodium dihydrogen phosphate, 1.2g carbomer, 2.4g levamisole, 4g propolis extract, 5g Astragalus polysaccharide, Polygonatum Polysaccharide 2g.

[0037] The preparation method of the vaccine diluent is: dissolving sodium chloride, potassium...

Embodiment 2

[0047] Embodiment 2: a kind of oral attenuated lyophilized vaccine for classical swine fever, comprising attenuated classical swine fever virus, lyoprotectant and vaccine diluent, is characterized in that, comprises mucosa immune adjuvant and immunopotentiator in the described vaccine diluent, so The mucosal immune adjuvant includes propolis extract, levamisole and carbomer, and the immune enhancer includes astragalus polysaccharide and polygonatum polysaccharide.

[0048] The lyoprotectant includes 3% bovine serum albumin BSA, 2% mannitol, 2% glycine, 6% lactose and 87% deionized water in mass percent.

[0049] Each liter of the vaccine diluent contains: 130mM sodium chloride, 3.0mM potassium chloride, 10mM disodium hydrogen phosphate, 2mM sodium dihydrogen phosphate, 1.5g of carbomer, 3.0g of levamisole, 2g of propolis extract , 3g of Astragalus Polysaccharide, 1.5g of Polygonatum polysaccharide.

[0050] The preparation method of the vaccine diluent is: dissolving sodium c...

Embodiment 3

[0060] Embodiment 3: a kind of oral attenuated lyophilized vaccine for classical swine fever, comprising attenuated classical swine fever virus, lyoprotectant and vaccine diluent, is characterized in that, comprises mucosa immune adjuvant and immunopotentiator in the described vaccine diluent, so The mucosal immune adjuvant includes propolis extract, levamisole and carbomer, and the immune enhancer includes astragalus polysaccharide and polygonatum polysaccharide.

[0061] The lyoprotectant includes 3% bovine serum albumin BSA, 2% mannitol, 2% glycine, 5% lactose and 88% deionized water in mass percent.

[0062] Each liter of the vaccine diluent contains: 130mM sodium chloride, 3.0mM potassium chloride, 10mM disodium hydrogen phosphate, 2mM sodium dihydrogen phosphate, 1.4g carbomer, 2.8g levamisole, 3g propolis extract, 2g Astragalus polysaccharide, Polygonatum Polysaccharide 1g.

[0063] The preparation method of the vaccine diluent is: dissolving sodium chloride, potassium...

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Abstract

The invention relates to an oral attenuated lyophilized vaccine for swine fever, which comprises a swine fever attenuated virus, a lyoprotectant and a vaccine diluent, wherein the vaccine diluent is prepared from a mucosal immune adjuvant and an immunopotentiator, and the mucosal immune adjuvant is prepared from a propolis extract, levamisole and carbomer; the immunopotentiator is prepared from astragalus polysaccharide and polygonatum polysaccharide. The oral attenuated lyophilized vaccine for the swine fever having a good immune effect is prepared by taking a attenuated strain of swine feveras a virus seed, and improving the lyoprotectant and the vaccine diluent, the vaccine can realize the complete protection of strong virulent virus of swine fever, generates higher antibody titer, hasbetter / comparative immune effects compared with commercially available inject vaccinate, and is convenient to use.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to an oral attenuated freeze-dried vaccine for swine fever and a preparation method thereof. Background technique: [0002] Classical swine fever (CSF) is an acute septicemia and highly contagious disease of pigs caused by classical swine fever virus (CSFV), characterized by high fever and diffuse hemorrhage of whole body tissues. Typical symptoms and pathological features. CSF once caused great economic losses to the swine industry all over the world. The World Organization for Animal Health stipulated it as an animal infectious disease that must be reported. my country has also designated swine fever as a severe infectious disease. animal diseases, and diseases that require urgent, strict and compulsory prevention. Although some countries have successfully decontaminated CSFV, it still occurs occasionally in most pig farming areas of the world, distributed in many countr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K47/42A61K47/26A61K47/18A61K39/187A61K39/39A61P31/14
CPCA61K9/19A61K39/12A61K39/39A61K47/183A61K47/26A61K47/42A61K2039/5254A61K2039/542A61K2039/552A61K2039/55511A61K2039/55583A61K2039/55588A61P31/14C12N2770/24334
Inventor 张志刚
Owner 张志刚
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