A kind of blue fluorescent quantum dot and its preparation method and copper ion detection application
A technology of blue fluorescence and quantum dots, applied in the field of nanomaterials, can solve the problems of reduced sensitivity of copper ions and reduced number of amino groups
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Embodiment 1
[0050] Example 1 Preparation of carbon quantum dots
[0051] The present invention used as a nitrogen-based polyethylenediamine dendritic polymer can be selected by commercially available products as a nitrogen source.
[0052] See figure 1 , Blue fluorescent quantum point NH of the present invention 2 -CDS preparation method, including the following steps:
[0053] Step 1: Preparation of G-PAMAM
[0054] 1) 4 mL of ethylenediamine and 10 mL of methanol were added to the round bottom flask and stirred for 5 min. After stirring, 16 ml of methyl acrylate was added dropwise to the above solution and reacted at 30 ° C for 24 h;
[0055] 2) After the reaction was completed, the methanol was removed by a rotary evaporator at 30 ° C, and three times were washed three times with methanol to remove a large amount of methyl acrylate. 10 ml of methanol was added to the above-mentioned solution and was dispersed uniform with ultrasound. A 16 mL of ethylenediamine was added dropwise to the dis...
Embodiment 2
[0065] Example 2 Characterization of carbon quantum dots
[0066] 1, average particle size and zeta potential characterization
[0067] Bare-CDS and NH were measured by Zen3600 Malvin Dynamic Light Scattering Granular Meter 2 -CDS's average particle size and Zeta potential of two samples, the test medium is water, and the test temperature is 25 ° C.
[0068] 2, TEM micro morphology and structural characterization
[0069] Take a small amount of Bare-CDS and NH 2The -CDS was added 2 ml of anhydrous methanol in a centrifuge tube and ultrasonic dispersion in a 5 ml of centrifuge tube, respectively, and the sample was uniformly dripped onto 400-mesh copper nets, and the sample morphology was observed under the 80 kV acceleration voltage of the TECNAi G2F20. Micro-morphological observations were performed using a transmission electron microscope (TEM), a dynamic light scattering particle meter (DLS), and Fourier infrared spectrometer (FT-IR). Micro-morphological observations were carri...
Embodiment 3
[0076] Example 3 Carbon dot detection copper ions
[0077] 1, copper ion responsive experiment
[0078] Due to NH 2 -CDS surface of the amino group can quickly capture the solution of the solution 2+ The copper ammonia complex is formed and covered with the surface thereof. According to the fluorescence internal rate effect, the formed copper ammonia complex is covered in NH. 2 -CDS surface. At this time, NH 2 The copper ammonia complex formed on the surface of the -CDS prevents it from receiving external ultraviolet stimulation, or when it receives foreign stimulation, it will prevent the emission of fluorescence. Therefore, when NH 2 -CDS capture Cu 2+ The copper ammonia complex formed thereof is changed to change its spectral behavior.
[0079] See Figure 4 , Figure 4 The A c e represents a carbon quantum point NH, respectively. 2 -CDS ultraviolet absorption spectroscopy, excitation spectrum and emission spectrum; B D f represent NH, respectively 2 -CDS and the copper ion bindi...
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