Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for ultra-low temperature removal of Jianlan mosaic virus and blue ringspot virus

A technology for blue tooth ringspot virus and orchid flower leaf building, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of short processing time, plant death, low virus removal efficiency, etc., and reduce the difficulty , reducing the scope and reducing the risk of browning

Active Publication Date: 2022-01-28
SOUTHWEAT UNIV OF SCI & TECH
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research results show that the low concentration of the agent and the short treatment time have little toxic effect on plants, but the virus removal efficiency is low. Toxic effects and even cause plant death
②Chemical agents can easily cause genetic variation of seedlings
[0005] At present, ultra-low temperature detoxification technology has not been applied to Jianlan mosaic virus and blue tooth ringspot virus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for ultra-low temperature removal of Jianlan mosaic virus and blue ringspot virus
  • A method for ultra-low temperature removal of Jianlan mosaic virus and blue ringspot virus
  • A method for ultra-low temperature removal of Jianlan mosaic virus and blue ringspot virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Take Phalaenopsis tissue-cultured seedlings (seedling age: 6 weeks) infected with Jianlan mosaic virus and blue ringspot virus as objects, under the condition of ultra-clean workbench, take 1.0-1.5cm stems under a stereo microscope tip; put the shoot tip into the pre-culture medium (BM+0.6M sucrose) for 2 days pre-cultivation (dark condition); the composition of BM medium (per liter) is: Huabao No. 1 3g+ sodium ferric edetate 10ml (the mother liquor is 3.67g dissolved in 1000ml) + organic matter 10ml (the mother liquor is glycine 0.2g + thiamine 1.0g + pyridoxine hydrochloride 0.1g + pantothenate calcium 0.1g + inositol 10g mixed solution in 1000ml) + peptone 1.5g; at room temperature Load the shoot tips with loading solution (BM+2M glycerol+0.4M sucrose) for 20 minutes; treat the shoot tips with cryoprotectant solution (PVS2 solution) for 40-50 minutes at 0°C; draw the PVS2 solution with a pipette gun, and put it on the aluminum foil Small droplets (about 2.5ul in drop...

Embodiment 2

[0048] Taking Phalaenopsis tissue-cultivated seedling variety 'Mantianhong' (seedling age: 6 weeks) infected with Jianlan mosaic virus and blue ringspot virus as the object, 1.5 cm-sized stem tip; put the stem tip into the pre-medium (BM+0.6M sucrose) for 1 day pre-cultivation; the composition of BM medium (per liter) is: Huabao No. 1 3g+ sodium iron ethylenediaminetetraacetate 10ml (100 times mother liquor is 3.67g dissolved in 1000ml distilled water) + organic matter 10ml (100 times mother liquor is glycine 0.2g + thiamine 1.0g + pyridoxine hydrochloride 0.1g + calcium pantothenate 0.1g + inositol 10g mixed solution in 1000ml distilled water) + peptone 1.5g + 30g sucrose + 7g agar, the pH value is 6.0; load the shoot tip with the loading solution (BM+2M glycerol+0.4M sucrose) at room temperature for 30 minutes; put the PVS2 vitrification solution (MS+30 % glycerol+15% ethylene glycol+15%DMSO+0.4M sucrose) is taken out, the PVS2 solution that gets 20ml is poured in the petri ...

Embodiment 3

[0050] Taking Phalaenopsis tissue culture seedling variety 'Diamond Queen' (seedling age: 6 weeks) infected with Jianlan mosaic virus and blue ringspot virus as the object, under the condition of ultra-clean workbench, take 1.0cm Stem tips of different sizes; put the shoot tips into the pre-culture medium (BM+0.6M sucrose) for 1 day pre-cultivation; the composition of BM medium (per liter) is: Huabao No. 1 3g+10ml of sodium ferric edetate (100 times mother liquor is 3.67g dissolved in 1000ml distilled water) + organic matter 10ml (100 times mother liquor is glycine 0.2g + thiamine 1.0g + pyridoxine hydrochloride 0.1g + calcium pantothenate 0.1g + inositol 10g mixed solution in 1000ml distilled water) + peptone 1.5 g+sucrose 30g+agar 7g, the pH value is 6.0; under room temperature conditions, load the shoot tips with loading solution (BM+2M glycerol+0.4M sucrose) for 25 minutes; the PVS2 vitrification solution (MS+30% Glycerol+15% ethylene glycol+15%DMSO+0.4M sucrose) was taken...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for removing Jianlan mosaic virus and Cauliflower ring spot virus. Take the shoot tip down; put the shoot tip into the pre-culture medium for pre-cultivation; load the shoot tip with the loading solution; treat the shoot tip with the cryoprotective solution; put the shoot tip treated with the cryoprotective solution into liquid nitrogen for ultra-low temperature treatment; The shoot tips after ultra-low temperature treatment were put into the unloading solution for unloading; the treated shoot tips were put into regeneration medium, and then transferred to normal light for cultivation; after 3 months, the shoot tips were successfully grown into seedlings. The invention can improve the survival rate and regeneration rate of the shoot tip.

Description

technical field [0001] The invention belongs to the technical field of detoxification, and in particular relates to a method for detoxifying Jianlan mosaic virus and blue tooth ringspot virus at ultra-low temperature. Background technique [0002] At present, 25 kinds of viruses have been reported to infect Phalaenopsis, among which Cymbidium mosaicvirus (CymMV) and Odontoglossum ringspot virus (ORSV) are the most common and the most harmful. Long-term asexual reproduction leads to the continuous accumulation of viruses in Phalaenopsis, and the lack of effective methods to prevent and control the infection and transmission of the two viruses makes Phalaenopsis generally infected with viruses, which has become one of the bottlenecks affecting the quality of Phalaenopsis seedlings. Many countries in the world, including all parts of my country, have reported that Phalaenopsis seedlings are infected with the two viruses and are extremely common. Jianlan mosaic virus causes chl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王仁睿李杰
Owner SOUTHWEAT UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com