In-situ forming injectable hydrogel for bone-cartilage integrated restoration

A technology for cartilage repair and in situ molding, which is applied in prosthesis, drug delivery, tissue regeneration, etc. It can solve the problems of unseen injectable bone repair hydrogel materials, achieve high degree of substitution, increase cross-linking density, The effect of mild reaction conditions

Active Publication Date: 2018-12-21
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are no reports on the preparation of in situ injectable bone repair hydrogel materials based on methacrylylated γ-polyglutamic acid and four-armed polyethylene glycol mercapto groups

Method used

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  • In-situ forming injectable hydrogel for bone-cartilage integrated restoration
  • In-situ forming injectable hydrogel for bone-cartilage integrated restoration
  • In-situ forming injectable hydrogel for bone-cartilage integrated restoration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Add 3.0g of γ-polyglutamic acid, 75mg of 4-dimethylaminopyridine and 3.3g of glycidyl methacrylate to 100mL of phosphate buffer solution with pH=8.0, and stir at 1000rpm for 36h at room temperature to cross-link in the dark. The solution was transferred to a dialysis bag with a molecular weight cut-off of 3500, dialyzed with deionized water for 48 hours (substances with a molecular weight less than 3500 could be removed), and then centrifuged at 5000 rpm for 10 minutes using a centrifuge. The supernatant was taken out and freeze-dried to obtain methacrylylated γ-polyglutamic acid. Dissolve methacrylylated γ-polyglutamic acid and four-armed polyethylene glycol thiol in phosphate buffer at pH = 8.0 to obtain methacrylylated γ-polyglutamic acid with a mass fraction of 10%. solution and 10% four-arm polyethylene glycol mercapto solution. Mix the above solution according to the mass ratio of 1:4, 1:7, 1:10 and 1:13 (methacrylylated γ-polyglutamic acid: four-armed polyethyle...

Embodiment 2

[0038] The methacrylylated γ-polyglutamic acid obtained in Example 1 was freeze-dried and ground into powder, and the substitution degree of the material was detected by proton nuclear magnetic resonance spectroscopy. Dissolve 5 mg of powder in 600 μL deuterated chloroform reagent, and detect its hydrogen spectrum with a 400 MHZ nuclear magnetic resonance instrument.

[0039] figure 1 It is the result of the methacrylylated γ-polyglutamic acid proton nuclear magnetic resonance spectrum experiment that embodiment 1 obtains, as seen in the figure, after GMA is grafted to the α-position carboxyl group of the γ-PGA side chain, 4.32ppm (H ,-CH-) spectrum peaks split; carbon-carbon double bond protons (2H,CH 2 =) characteristic peak, indicating that the grafting was successful, and the degree of substitution was 7.2%.

Embodiment 3

[0041] The injectable hydrogel precursor solution obtained in Example 1 was dropped onto the stage of the rheometer, and the rheological behavior of the precursor solution was detected in an oscillation mode. The detection temperature is 37°C, the oscillation frequency is 1HZ, and the test interval is 100μm.

[0042] figure 2 It is the result of the rheological experiment of the injectable hydrogel precursor solution obtained in Example 1. As can be seen from the figure, at 37°C, the phase transition times of IBRH1:4, IBRH1:7, IBRH1;10 and IBRH1:13 were 960s, 870s, 860s, and 780s, respectively.

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PUM

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Abstract

The invention discloses an in-situ forming injectable hydrogel for bone-cartilage integrated restoration. The in-situ forming injectable hydrogel is prepared by the following steps of using gamma-PGA(gamma-plyglutamic acid) and GMA (glycidyl methacrylate) as the main raw materials, and generating methylacrylated gamma-PGA under the catalyzing function of DMAP (4-dimethylaminopyridine); uniformlymixing the mPGA and 4 arm PEG SH (four-arm polyethylene glycol mercapto), and forming in situ, so as to obtain the in-situ forming injectable hydrogel. The in-situ forming injectable hydrogel has goodinjectability, in-vitro forming property, biocompatibility and degradability, and has larger application potential in the field of restoration of bone and cartilage irregularity lesions.

Description

technical field [0001] The invention belongs to the field of biomedical polymer materials and the cross field of organic chemistry, and more specifically relates to an in-situ molded injectable hydrogel for bone-cartilage comprehensive repair. Background technique [0002] Bone and cartilage tissue defect is a secondary damage commonly seen in bone trauma, bone tuberculosis, and bone tumor. It has the characteristics of high incidence, long course of disease, and many complications. Usually, small-scale bone and cartilage tissue defects can be repaired compensatoryly by the body, and large-scale lesions can be reconstructed with autologous bone graft, allogeneic bone graft, and artificial bone tissue engineering scaffold. Autologous bone grafting is an internationally recognized gold standard for bone repair, and it is effective in integrating and repairing damaged tissues. However, the bone grafts that can be provided by autologous bone grafting are very limited, and it wi...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/50A61L27/52A61L27/58C08J3/075C08J3/24C08L71/02C08L77/04
CPCA61L27/18A61L27/50A61L27/52A61L27/58A61L2400/06A61L2430/02A61L2430/06C08J3/075C08J3/246C08J2377/04C08J2471/02C08L77/04C08L71/02
Inventor 王江林胡伟康王子健徐小蕾王行环肖宇
Owner HUAZHONG UNIV OF SCI & TECH
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