Method for preparing glycoprotein molecular surface imprinted polymers on basis of dendritic boric acid and application of glycoprotein molecular surface imprinted polymers

A technology of surface imprinting and glycoprotein, which is applied in the field of preparation of dendritic boronic acid as a functional monomer to construct efficient boron affinity imprinting polymers, which can solve the problems of inability to achieve selective separation, weak boron affinity sites, biomacromolecules Embedding depth and other issues to achieve the effect of weakening non-specific adsorption, enhancing adsorption selectivity, and enhancing hydrophilicity

Active Publication Date: 2018-12-25
广东健医师生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In this work, in order to solve the defect that the single boron affinity site is weak and cannot achieve selective separation of target glycoproteins, surface imprinting technology is further introduced on the basis of multiple covalent bond synergy
Surface imprinting technology is an innovation of molecular imprinting technology in the field of biomacromolecules. The imprinting cavity is close to or distributed on the surface of the matrix material, which can fully solve the defects of deep embedding, difficult elution and slow mass transfer of biomacromolecules caused by traditional imprinting methods.

Method used

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  • Method for preparing glycoprotein molecular surface imprinted polymers on basis of dendritic boric acid and application of glycoprotein molecular surface imprinted polymers
  • Method for preparing glycoprotein molecular surface imprinted polymers on basis of dendritic boric acid and application of glycoprotein molecular surface imprinted polymers
  • Method for preparing glycoprotein molecular surface imprinted polymers on basis of dendritic boric acid and application of glycoprotein molecular surface imprinted polymers

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Experimental program
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Effect test

Embodiment 1

[0038] (1) SiO 2 preparation of

[0039] Mix 8.74g tetraethyl orthosilicate (TEOS) with 180mL absolute ethanol, and stir in a water bath at 35°C, then slowly add a mixed solution of 10mL ammonia water and 9.46g double-distilled water, and react for 12h. After the reaction, the obtained SiO 2 Nanoparticles were separated by centrifugation and washed several times with absolute ethanol and double distilled water, and finally the washed particles were dispersed in 10 mL of absolute ethanol for later use.

[0040] (2) Amino modified SiO 2 Nanoparticles (SiO 2 -NH 2 ) preparation

[0041] Add 2mL of 3-aminopropyltriethoxysilane (APTES) into a 100mL three-necked flask, and then add the SiO obtained in step (1) 2 The dispersion liquid and 50mL of absolute ethanol were dispersed uniformly by ultrasonic, and then the flask was placed in a 50°C water bath to react overnight. After the reaction, the product was washed several times with absolute ethanol and then used for later use...

Embodiment 2

[0051] (1) SiO 2 preparation of

[0052] Consistent with step (1) in embodiment column 1;

[0053] (2) Amino modified SiO 2 Nanoparticles (SiO 2 -NH 2 ) preparation

[0054] Consistent with step (2) in the embodiment row 1;

[0055] (3) Aldehyde modified SiO 2 Nanoparticles (SiO 2 -CHO) preparation

[0056] Consistent with step (3) in embodiment column 1;

[0057] (4) PEI grafted SiO 2 (SiO 2 -PEI) preparation

[0058] Weigh 1g of polyethylene polyamine (PEI) and 0.8g of SiO 2 -CHO nanoparticles were added into a 100mL three-neck flask, followed by 50mL of methanol, dispersed uniformly by ultrasound, reacted at room temperature for 8h, and added 30mg of sodium borohydride every 4 hours. After the reaction, the product was centrifuged, washed three times with absolute ethanol and double distilled water, and finally dried in a vacuum oven at 45°C for 12 hours.

[0059] (5) branched boric acid polymer SiO 2 Preparation of @FPBA:

[0060] First, for SiO 2 @PEI nan...

Embodiment 3

[0064] (1) SiO 2 preparation of

[0065] Consistent with step (1) in the implementation column 1

[0066] (2) Amino modified SiO 2 Nanoparticles (SiO 2 -NH 2 ) preparation

[0067] Consistent with step (2) in the embodiment row 1

[0068] (3) Aldehyde modified SiO 2 Nanoparticles (SiO 2 -CHO) preparation

[0069] Consistent with step (3) in the embodiment row 1

[0070] (4) PEI grafted SiO 2 (SiO 2 -PEI) preparation

[0071] Weigh 5g of polyethylene polyamine (PEI) and 1.5g of SiO 2 -CHO nanoparticles were added into a 100 mL three-necked flask, followed by 50 mL of methanol, dispersed uniformly by ultrasonic, and reacted at room temperature for 8 hours, and 100 mg of sodium borohydride was added every 4 hours. After the reaction, the product was centrifuged, washed three times with absolute ethanol and double distilled water, and finally dried in a vacuum oven at 45°C for 12 hours.

[0072] (5) branched boric acid polymer SiO2 Preparation of @FPBA:

[0073] Fir...

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Abstract

The invention belongs to the technical field of preparation of biomedical functional high-polymer materials, and particularly discloses a method for preparing glycoprotein molecular surface imprintedpolymers on the basis of dendritic boric acid and application of the glycoprotein molecular surface imprinted polymers. The method and the application have the advantages that the dendritic boric acidis used as a recognition monomer, and accordingly the OVA adsorption affinity and contact probability of the SiO<2>-MIPs (SiO<2>-glycoprotein molecular surface imprinted polymers) can be effectivelyenhanced by multiple covalent bond synergistic effects of the dendritic boric acid as compared with single-stranded boric acid; the shortcoming of weak hydrophilicity of the traditional boric acid canbe effectively overcome by the aid of the dendritic boric acid, dopamine is used as an imprinted layer, the hydrophilicity of adsorbents can be effectively enhanced, accordingly, non-specific adsorption due to hydrophobic effects can be weakened, and the adsorption selectivity can be enhanced; affinity effects of boron can be controlled by means of environmental pH (potential of hydrogen) regulation, and accordingly OVA can be controllably separated in pH regulation modes; in conclusion, the SiO<2>-MIPs with the dendritic boric acid and surface imprinting technologies are easy and convenientto prepare and high in selectivity and affinity, so that the method has a huge prospect in the field of glycoprotein separation and enrichment.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical functional polymer materials, and relates to a method for preparing an imprinted polymer suitable for selectively separating glycoproteins, in particular to a dendritic boronic acid used as a functional monomer to construct a high-efficiency boron-affinity imprinted polymer Preparation method and application. Background technique [0002] Protein glycosylation plays a key role in many life activities. In addition, abnormal glycoproteins have long been considered to be closely related to the occurrence of various cancers. Large-scale analysis of these glycoproteins not only helps to discover new diagnostic markers, but also provides important information for clinical treatment. However, the inherent low abundance of glycoproteins in biological samples accompanied by a large number of interfering substances has caused great difficulties for the analysis of glycoproteins. Therefo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/30C07K14/77C07K1/14
CPCB01J20/103B01J20/22B01J20/24B01J2220/4806B01J2220/4812B01J2220/4856C07K14/77
Inventor 夏可旭潘建明朱恒佳刘金鑫
Owner 广东健医师生物科技有限公司
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