Pleurotus citrinopileatus germ bran polysaccharide and extraction process and application thereof

A kind of elmia fungus, extraction process technology, applied in the directions of medical preparations containing active ingredients, digestive system, organic active ingredients, etc., can solve the problem of not further improving the utilization value, etc., and achieve high extraction rate and excellent biological activity. Effect

Active Publication Date: 2019-01-18
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Pleurotus citrinopileatus germ bran polysaccharide and extraction process and application thereof
  • Pleurotus citrinopileatus germ bran polysaccharide and extraction process and application thereof
  • Pleurotus citrinopileatus germ bran polysaccharide and extraction process and application thereof

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preparation example Construction

[0066] Preparation of standard glucose solution: Accurately weigh 0.0100g of glucose (dried to constant weight in a constant temperature oven at 105°C before use) into a 100mL volumetric flask, and constant volume to obtain a 100μg / mL glucose standard solution.

[0067] Preparation of standard curve: Accurately measure 0.2mL, 0.4mL, 0.6mL, 0.8mL, 1.0mL, 1.2mL of glucose standard solution into a stoppered test tube, add water to make up to 2.0mL, add 1mL of 6% phenol solution, shake well, Quickly add 5mL of concentrated sulfuric acid, shake well, and let stand for 26min. Take 2.0mL of deionized water as a blank, measure the absorbance at a wavelength of 490nm, take the absorbance OD490nm as the ordinate, and the reference substance solution mass concentration C (μg / mL) as the abscissa, and make a standard curve, such as figure 1 As shown, the regression equation y=0.0147x-0.0137 is obtained. Regression coefficient R 2 =0.9984, the equation is available.

[0068] Determinatio...

Embodiment 1

[0070] A kind of extraction process of furfur polysaccharide of ulm yellow fungus, this process comprises the following steps:

[0071] (1) Expose the chaff of the yellow fungus to the sun for 1-2 days, after preliminary drying (the water content is about 10%-20%), put it in an oven at 60°C and dry it to constant weight, crush it, and pass it for 10-20 Mesh sieve;

[0072] (2) Add the sifted chaff powder to water according to the material-to-liquid ratio of 1:40g / mL, the extraction temperature is 85°C, the extraction times are 1 time, the extraction time is 1h, and the pH is 5. After extraction, the extract is obtained and concentrated to half of its original volume;

[0073] (3) Add an ethanol solution with a concentration of 84 (v / v)% to the concentrated extract, the added volume is 3 times of the extract, the alcohol precipitation time is 12h, the alcohol precipitation temperature is -4°C, and the precipitate is collected. Add 1g of the precipitate to 3mL of a mixture of ...

Embodiment 2

[0075] A kind of extraction process of furfur polysaccharide of ulm yellow fungus, this process comprises the following steps:

[0076] (1) Expose the fungus chaff of the yellow fungus to the sun for 1-2 days, after drying, crush it, and pass it through a 10-20 mesh sieve;

[0077] (2) Add the sifted chaff powder to water according to the material-to-liquid ratio of 1:40g / mL, the extraction temperature is 86°C, the extraction time is 1 time, the extraction time is 1h, and the pH is 5. After extraction, the extract is obtained and concentrated to half of its original volume;

[0078] (3) Add an ethanol solution with a concentration of 85 (v / v)% to the concentrated extract, the volume of which is 4 times that of the extract, the alcohol precipitation time is 12 hours, the alcohol precipitation temperature is 4° C., and the precipitate is collected. Add 1g of the precipitate to 3mL of a mixture of absolute ethanol, ether, and acetone (volume ratio 3:2:1) and centrifuge at 3200r / ...

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Abstract

The present invention relates to a pleurotus citrinopileatus germ bran polysaccharide and an extraction process and an application thereof. The pleurotus citrinopileatus germ bran polysaccharide extracted by the extraction process under specific conditions by taking pleurotus citrinopileatus germ bran as a raw material is not only high in extraction rate, but also excellent in reducing the elevation of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase caused by acute liver injury, as well as improving drugs for albumin decrease caused by acute liver injury, and increasing the superoxide dismutase activity, catalase activity, lipid peroxide content, and/or malondialdehyde decreased biological activity caused by acute liver injury.

Description

technical field [0001] The present disclosure specifically relates to a furfur polysaccharide from the yellow fungus ulmus and its extraction process and application. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] Pleurotus citrinopileatus belongs to Eumycophyta, Basidiomycotina, Hymenomycetes, Agaricales, Pleurotaceae, Pleurotaceae (Pleurotaceae) in taxonomy. Pleurotus). Yellow elm mushroom is not only rich in nutrition and high in economic value, but also rich in various biologically active substances such as carbohydrates, proteins, trace elements, enzymes, dietary fiber and vitamins. Ulmus chrysanthemum chaff is the substrate residue after harvesting of ulm chrysanthemum, commonly known as edible fungus cultivation waste, fungus residue or residue, its main components are sawdust, straw, etc., which are fermented by enzymes secreted by ulm...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P1/16
CPCA61K31/715A61P1/16C08B37/0003
Inventor 贾乐张建军杨启航刘新超
Owner SHANDONG AGRICULTURAL UNIVERSITY
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