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Method for detecting isoflavones compound

A compound and isoflavone technology, applied in the field of analytical chemistry, can solve problems such as unsatisfactory separation, consumption, and long-term consumption, and achieve the effects of reducing impurity interference, no need for purification process, and fast analysis speed

Active Publication Date: 2019-01-22
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods often suffer from high organic reagent consumption and long time consumption (approximately 30-90 minutes), the latter being the main bottleneck when high-throughput analysis is required
UHPLC columns have shorter analysis times (12 minutes or less), but some structurally similar isoflavones are not well resolved due to the same separation mechanism

Method used

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  • Method for detecting isoflavones compound
  • Method for detecting isoflavones compound
  • Method for detecting isoflavones compound

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Embodiment 1

[0041] Utilize the method for the embodiment of the present invention to detect isoflavones, and compare and analyze the experimental conditions, specifically as follows:

[0042] 1. Raw materials and instruments

[0043] 1.1 Standards, Reagents and Samples

[0044] Puerarin, mirificin, 6-O-xyloside puerarin, 4'-O-methylpuerarin, 3'-methoxypuerarin, daidzein, daidzin, formononetin, ononin, genistein , genistin, biotin A, glycitein, glycine, irisaxanthin and tectoridin were purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). All 16 compounds were more than 98% pure. Their accurate masses were confirmed by a Quadrupole-Orbitrap hybrid mass spectrometer (ThermoScientific, San Jose, CA, USA).

[0045] LC grade methanol, formic acid and oxalic acid were purchased from Sigma-Aldrich (St. Qentin Fallavier, France). Water was purified using the Milli-Q-System (Millipore, Guyancourt, France). Food grade carbon dioxide was purchased from Beijing Green Oxygen...

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Abstract

The invention discloses a method for detecting an isoflavones compound. The method comprises the following steps: extracting a to-be-detected sample by using an alcoholic solution so as to obtain an extracting solution; and using an ultra-high performance supercritical fluid chromatography-tandem mass spectrometry to detect the extracting solution, so as to obtain the content of the isoflavones compound. The method is simple to operate, good in reproducibility, high in analysis speed and high in sensitivity, and baseline separation is realized; and besides, quantitative analysis and identification can be carried out on various common isoflavone components.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for detecting isoflavone compounds. Background technique [0002] Isoflavones are natural compounds with a common 3-phenyl-chromen-4-one skeleton. When comparing the structure of isoflavones with conventional flavonoids, the difference is that the phenyl group of the former is substituted at C-3 of the chromene moiety, while that of the latter is substituted at the C-2 position. Due to their structural similarity to estradiol, isoflavones, as phenolic phytoestrogens, play an important role in modulating estrogen receptors, exhibiting estrogenic and antiestrogenic effects. Therefore, isoflavones have been widely used as a remedy for the prevention of age-related and estrogen-dependent diseases such as menopausal syndrome. In addition to their hormone-like activity, isoflavones also possess other pharmacological activities, such as antifungal, antibacterial, antioxida...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 张峰许秀丽吴文杰王秀娟
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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