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Preparation method of novel multifunctional Alaska pollock polypeptide modified by taurine

A multifunctional, taurine technology, applied in the biological field, can solve problems such as waste of resources and environmental pollution, and achieve the effects of being beneficial to the body's absorption, reducing production costs, and shortening synthesis time.

Active Publication Date: 2019-02-19
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In processed fish food, there will be a lot of leftovers. Usually only a small part of fish skin, fish head and cod bone is processed into feed, and most of them are directly discarded, which causes environmental pollution while wasting resources.

Method used

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  • Preparation method of novel multifunctional Alaska pollock polypeptide modified by taurine
  • Preparation method of novel multifunctional Alaska pollock polypeptide modified by taurine
  • Preparation method of novel multifunctional Alaska pollock polypeptide modified by taurine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A novel taurine-modified Alaska cod multifunctional peptide, which contains:

[0034] a, natural polypeptide, its amino acid sequence is shown in SEQ ID NO.1;

[0035]b, the substituents bound to the polypeptide defined in a and introduced for modification; the above substituents are taurine. Taurine is a multifunctional active substance. By using taurine to modify the Alaska cod polypeptide with a single antioxidant activity, a new multifunctional peptide with both the biological activity of taurine and the antioxidant activity of Alaska cod polypeptide can be obtained. Anti-oxidation, scavenging free radicals, promoting brain development and other biologically active functions.

[0036] The natural peptide is the hydrolyzed protein product of Alaska cod. The natural polypeptide is isolated from the skeleton protein of Alaska cod, with a molecular weight of 672u and a sequence of Leu-Pro-His-Ser-Gly-Tyr, which has good antioxidant properties and high free radical sca...

Embodiment 2

[0046] A method for preparing a taurine-modified novel Alaska cod multifunctional peptide, the specific preparation steps are as follows:

[0047] 1) Dissolve 15 times the amount of the natural polypeptide in a sodium carbonate solution with a concentration of 15%, then add 12 times the amount of the polypeptide, an acetone solution containing fluorenylmethylcarbonyl chloride and an accelerator, and stir in an ice bath at -2°C for 25 minutes , then stirred and reacted at 20°C for 2 hours, extracted with ether for 3 times, adjusted the pH to 3 with concentrated hydrochloric acid, and precipitated, extracted with ethyl acetate for 3 times, dried over anhydrous magnesium sulfate, and precipitated with petroleum ether The protective peptide fragment can be obtained by precipitation. The weight ratio of the above-mentioned fluorenylmethylcarbonyl chloride in the acetone solution is 33%, and the weight ratio of the anthranilic acid and isonicotinamide in the accelerator is 0.1% in th...

Embodiment 3

[0051] A method for preparing a taurine-modified novel Alaska cod multifunctional peptide, the specific preparation steps are as follows:

[0052] 1) Dissolve 13 times the amount of the natural polypeptide in a sodium carbonate solution with a concentration of 12%, then add 11 times the amount of the polypeptide, an acetone solution containing fluorenylmethylcarbonyl chloride and an accelerator, and stir in an ice bath at 0°C for 30 minutes. Then stirred and reacted at 25°C for 2.25h, extracted twice with diethyl ether, adjusted the pH to 3 with concentrated hydrochloric acid, and precipitated, extracted twice with ethyl acetate, dried over anhydrous magnesium sulfate, and precipitated with petroleum ether Precipitate to obtain protective peptide fragments, the weight ratio of the above-mentioned fluorenylmethylcarbonyl chloride in the acetone solution is 28%, and the weight ratio of anthranilic acid and isonicotinamide in the accelerator is 0.085% in the acetone solution respe...

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Abstract

The invention provides a preparation method of novel multifunctional Alaska pollock polypeptide modified by taurine. The multifunctional polypeptide includes natural polypeptide and a substituent group, wherein the amino acid sequence of the natural polypeptide is SEQ ID NO.1, and taurine is adopted as the substituent group which is combined with the natural polypeptide and introduced for modification. The preparation method of the polypeptide includes preparation of protective peptide fragments, a microwave condensation reaction and a deamination protection reaction. During preparation of theprotective peptide fragments, the natural polypeptide is dissolved in a sodium carbonate solution, an acetone solution containing amino-terminal protection groups and an accelerant is added, stirringis conducted for a reaction, and extraction and precipitation are carried out to obtain the fragments, wherein the accelerant comprises o-aminobenzoic sulfonic acid and isonicotinamide. The providednovel polypeptide has high activity and stability, no spiral structure, high fluidity and a high swelling ratio and can achieve biological activities such as oxidation resistance, removal of free radicals and promotion of brain development. The conversion rate and the yield are high during preparation. The time consumed for synthesis is short, the yield of by-products is low, products are easy topurify, and the production cost is low.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of a novel multifunctional peptide of Alaska cod modified with taurine. Background technique [0002] Cod, also known as cod, is one of the main edible fish. Cod is native to the cold waters of the North Atlantic from northern Europe to Canada and the eastern United States. At present, the main producing countries of cod are Canada, Iceland, Norway and Russia, and the main producing countries of Japan are Hokkaido. China is produced in the Yellow Sea and the northern part of the East China Sea. The main fishing grounds are in the northern part of the Yellow Sea, the east-southeast of Gaojiao in Shandong, the southern part of Haiyang Island and the southeast sea area. Cod is one of the fish with the largest annual catch in the world and has important economic value. Cod is an important economic fish for the production of surimi products, and its in...

Claims

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Application Information

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IPC IPC(8): C07K1/107C07K1/02
CPCC07K1/026C07K1/1077Y02P20/55
Inventor 金火喜徐红萍谢辉曾明曲有乐
Owner ZHEJIANG OCEAN UNIV
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