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Purification process of chlorogenic acid in corn vinasse

A technology of corn distiller's grains and chlorogenic acid, which is applied in the separation/purification of carboxylate, organic chemistry, carboxylate preparation, etc. It can solve the problems of high cost and low purity of chlorogenic acid, reduce the dosage and improve the crushing Efficiency and time-saving effect

Inactive Publication Date: 2019-02-22
JILIN AGRI SCI & TECH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Because chlorogenic acid has good activity, people have extracted chlorogenic acid from various plants (such as eucommia and honeysuckle), but its cost is relatively high, and the purity of the extracted chlorogenic acid is relatively low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A process for purifying chlorogenic acid in corn distiller's grains, comprising the steps of:

[0026] S1. Clean the distiller's grains, put them in a constant temperature drying oven, dry them at a temperature of 70°C until they reach a constant weight, and then perform ultrafine grinding and sieving, and the obtained distiller's grains are recorded as scraps of corn distiller's grains;

[0027] S2. Soak the corn distiller's grains scrap obtained in S1 in distilled water at a temperature of 28°C, and add a compound enzyme whose mass is 3% of the distilled water mass to the distilled water, mix and stir evenly, let stand for 5 hours, and ultrasonically disperse for 10 minutes every 30 minutes , the result is recorded as the mixed component;

[0028] S3. Raise the temperature of the mixed components to 50°C, and add a calcium ion solution with a mass of 20% of the mixed components and a concentration of 2mol / L to it, mix and stir evenly, let it stand for 50min, and then ...

Embodiment 2

[0040] A process for purifying chlorogenic acid in corn distiller's grains, comprising the steps of:

[0041] S1. Clean the distiller's grains, put them in a constant temperature drying oven, dry them at a temperature of 75°C until they reach a constant weight, and then perform ultrafine grinding and sieving, and the obtained distiller's grains are recorded as scrap corn distiller's grains;

[0042] S2. Soak the corn distiller's grains obtained in S1 in distilled water at a temperature of 30°C, and add a compound enzyme whose mass is 4% of the mass of distilled water to the distilled water, mix and stir evenly, let stand for 6 hours, and ultrasonically disperse for 15 minutes every 30 minutes , the result is recorded as the mixed component;

[0043] S3. Raise the temperature of the mixed components to 55°C, and add a calcium ion solution with a mass of 25% of the mixed components and a concentration of 2.5mol / L to it, mix and stir evenly and let stand for 55min, then turn it t...

Embodiment 3

[0055] A process for purifying chlorogenic acid in corn distiller's grains, comprising the steps of:

[0056] S1. Clean the distiller's grains, put them in a constant temperature drying oven, dry them at a temperature of 80°C until they reach a constant weight, and then perform ultrafine grinding and sieving, and the obtained distiller's grains are recorded as scraps of corn distiller's grains;

[0057] S2. Soak the corn distiller's grain scraps obtained in S1 in distilled water at a temperature of 32°C, and add a compound enzyme whose mass is 5% of the distilled water mass to the distilled water, mix and stir evenly, let stand for 7 hours, and ultrasonically disperse for 15 minutes every 30 minutes , the result is recorded as the mixed component;

[0058] S3. Raise the temperature of the mixed components to 65°C, and add a calcium ion solution with a mass of 30% of the mixed components and a concentration of 3mol / L to it, mix and stir evenly and let it stand for 65 minutes, t...

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PUM

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Abstract

The invention relates to the technical field of chlorogenic acid purification, in particular to a purification process of chlorogenic acid in corn vinasse. The purification process comprises the stepsthat under the action of ultrasonic dispersion, cellulose, polygalacturonase and a protein Zeah can all be evenly dispersed in a mixed component, the contact probability between the cellulose, the polygalacturonase as well as a the protein Zeah and corn cell walls is increased to the great extent, and thus the enzymolysis efficiency on the corn cell walls is improved; the permeability of corn cells is increased under the action of calcium ions, and through cooperative use of an ultrasonic cell crusher, the crushing efficiency of the corn cells is improved greatly; under cooperative use actionof complex enzymes, ultrasonic dispersion, a calcium ion solution and the ultrasonic cell crusher, the chlorogenic acid flows out to the maximum limit; and then the chlorogenic acid is preliminarilypurified, namely lixiviated, through an ethanol solution, a crude chlorogenic acid product is prepared, then the crude chlorogenic acid product is eluted through ethyl acetate, finally, the chlorogenic acid is recrystallized through an organic solvent, and thus the purity of the prepared chlorogenic acid is improved, and yield of the prepared chlorogenic acid is increasedpurity and yield of the prepared chlorogenic acid are increased.

Description

technical field [0001] The invention relates to the technical field of chlorogenic acid purification, in particular to a process for purifying chlorogenic acid in corn distiller's grains. Background technique [0002] Chlorogenic acid is also called coffee tannin. That is, 3-caffeoylquinic acid, a chemical preparation extracted from the dried flower buds or flowers of Lonicera Lonicera Lonicera, as a raw material for medicines, health products, and cosmetics, as well as injections and injections. Chlorogenic acid has a choleretic effect and can increase bile secretion in rats. It is sensitizing to humans, and after inhalation of plant dust containing this product, asthma and dermatitis may occur. Chlorogenic acid also has a wide range of antibacterial effects, but it can be inactivated by proteins in the body. Similar to caffeic acid, it can increase the central excitability of rats when taken orally or intraperitoneally. It can increase the intestinal motility of rats a...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48C07C67/58C07C67/56C07C67/52
CPCC07C67/48C07C67/52C07C67/56C07C67/58C07C2601/14C07C69/732
Inventor 常桂英于加平
Owner JILIN AGRI SCI & TECH COLLEGE
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