Preparation method and application of polyphenol extracts in different states of palm fruit

A palm fruit and extract technology, applied in application, food extraction, medical preparations containing active ingredients, etc., can solve the problems of limiting polyphenol extraction rate and bioavailability rate, and achieve a promising market prospect and enhanced protection ability. , the effect of broad market prospects

Inactive Publication Date: 2019-03-01
KUNMING UNIV OF SCI & TECH
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AI Technical Summary

Problems solved by technology

[0005] Polyphenols are a class of natural compounds that widely exist in plants. Polyphenols in plants generally exist in three forms: free state, esterified state, and bound state. Due to the presence of a large amount of cellulose and cell walls in plants, these polyphenols are greatly limited. Extraction rate and bioavailability of polyphenols in different states

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  • Preparation method and application of polyphenol extracts in different states of palm fruit
  • Preparation method and application of polyphenol extracts in different states of palm fruit
  • Preparation method and application of polyphenol extracts in different states of palm fruit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: The preparation method of the polyphenol extracts in different states of this palm fruit is as follows:

[0028] (1) Chop the palm fruit and remove the core, put it into a vacuum bag, vacuum seal it, put it into an ultra-high pressure treatment kettle, and treat it at 500MPa for 10 minutes; take out the processed pulp, freeze-dry it, and grind it into powder;

[0029](2) After degreasing the powder in step (1), add methanol-acetone mixed solution (methanol solution with a mass concentration of 70% and a mass concentration of 70% % acetone solution mixed according to the volume ratio of 1:1), after mixing, ultrasonic extraction was performed for 30 minutes, the ultrasonic power was 200W, filtered, and the filter residue was repeatedly extracted twice, the combined filtrate was collected and the organic reagent was removed by rotary evaporation, and the aqueous phase was collected It is used for the extraction of free and esterified polyphenols; the extracti...

Embodiment 2

[0030] Embodiment 2: the preparation method of this palm fruit different states polyphenol extract is as follows:

[0031] (1) Chop the palm fruit and remove the core, put it into a vacuum bag, vacuum seal it, put it into an ultra-high pressure treatment kettle, and treat it at 300MPa for 10 minutes; take out the processed pulp, freeze-dry it, and grind it into powder;

[0032] (2) After degreasing the powder in step (1), add methanol-acetone mixture (80% methanol solution and 80% mass concentration % acetone solution mixed according to the volume ratio of 1:2), after mixing, ultrasonically extract for 25min, the ultrasonic power is 300W, filter, and repeat the extraction of the filter residue 3 times, collect and combine the filtrate to remove the organic reagent, and collect the water phase It is used for the extraction of free and esterified polyphenols; the extraction of free polyphenols is to adjust the pH of the aqueous phase to 3 with 6mol / L hydrochloric acid, and then ...

Embodiment 3

[0033] Embodiment 3: the mensuration of total phenolic content of palm fruit different extracts before and after ultrahigh pressure

[0034] The determination was carried out by the Folin phenol method, and the specific operation was as follows: each extract was dissolved in methanol and diluted to 1.0 mg / mL; 1 mL of the extract solution and 1 mL of Folin phenol reagent were mixed; after standing for 1 minute, 1.5 mL of Na 2 CO 3 ; Dilute to 10 mL with distilled water. 70°C water bath for 10 minutes, after cooling to room temperature, measure the absorbance value at 765nm, the content of total phenols is calculated by gallic acid calibration; the results are as follows figure 1 As shown, the total phenolic content of each extract obtained without ultra-high pressure treatment was (mg gallic acid / g palm fruit): free polyphenol 81.20±6.07 mg / g, esterified polyphenol 163.06±3.06 mg / g, The bound polyphenols were 220.49±14.43 mg / g, and the total phenolic content of polyphenol ext...

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Abstract

The invention discloses a preparation method of polyphenol extracts in different states of palm fruit, and belongs to the technical field of food or natural medicine. The preparation method comprisesthe steps that firstly super high pressure is adopted to perform pre-processing on a palm fruit, and then extracting is carried out under assistance of ultrasonic wave, and finally the polyphenol extracts in the different states of the palm fruit are obtained after extraction, rotary evaporation and drying; compared with a traditional extraction method, a total phenol content measurement result shows that super high pressure treatment can extremely and significantly improve the extraction rate of phenolic substances and the available rate of the polyphenol extracts in the different states of the palm fruit, and meanwhile, the bioactivity of the polyphenol extracts in three different three states of the palm fruit is all improved significantly verified by experiments, the inhibiting rate ofpancreatic lipase activity, and the inhibiting rate of <alpha>-glycosidase activity and the protective capability of cell oxidative damage are all significantly improved by the polyphenol extracts ofthe palm fruit. The polyphenol extracts of the palm fruit referred have the functions of better lipid lowering and weight losing, blood sugar reducing and prevention and treatment of body oxidative damage, and can be used for preparing relevant health food or medicine.

Description

technical field [0001] The invention relates to a preparation method of polyphenol extracts in different states of palm fruit and its application in foods or medicines related to diseases such as reducing fat and losing weight, lowering blood sugar, and preventing and treating oxidative damage to human tissue, and belongs to the technical field of food or natural medicines . Background technique [0002] In modern society, with rich material conditions and accelerated pace of life, unhealthy high-fat and high-carbohydrate diet and bad living habits have induced the occurrence of many chronic diseases, such as obesity, hyperlipidemia and diabetes, which seriously threaten people's health. Good health reduces people's standard of living. In the daily diet, the fat and carbohydrates in the food need to be hydrolyzed by the corresponding digestive enzymes in the gastrointestinal tract before they can be absorbed by the body. Studies have shown that about 70% of the fat in the f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/889A61P3/06A61P3/04A61P3/10A61P39/06A23L33/105
CPCA23V2002/00A61K36/889A61K2236/333A61K2236/39A61K2236/55A23L33/105A61P3/04A61P3/06A61P3/10A61P39/06A23V2200/3262A23V2200/3324A23V2200/328A23V2200/30A23V2300/14A23V2250/2132
Inventor 蔡圣宝周洁心王春丽李文婷马爽曾内辉
Owner KUNMING UNIV OF SCI & TECH
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