Method for preparing drug-carrying polyester polymer/biological ceramic bone repairing scaffold through low-temperature 3D printing technology as well as product and application thereof

Abstract

The invention relates to a method for preparing a polyester polymer / nano-biological ceramic bone repairing scaffold through a low-temperature 3D printing technology as well as a product and application thereof. The method comprises the following steps: using physical property of a polar solvent hexafluoroisopropanol, taking a hexafluoroisopropanol solution mixed with drug, polyester polymer and biological ceramic as 3D printing ink, printing till a product is solidified and molded on a low-temperature platform, then carrying out freeze-drying, heating and volatilizing to remove the solvent, soas to complete preparation of an artificial bone repairing scaffold. The preparation method provided by the invention is simple and practicable, provides a new method for clinical individualized treatment on massive bone defects, and has extensive clinical application prospects.

Description

technical field

[0001] The present invention relates to the technical field of biomedical materials, in particular to a method, product and application of a low-temperature 3D printing technology for preparing a drug-loaded polyester polymer / bioceramic bone repair scaffold, using hexafluoroisopropanol as a solvent and adopting low-temperature 3D printing Technology to prepare drug-loaded polyester polymer / bioceramic bone repair scaffold. Background technique

[0002] The repair of large bone defects is a major problem in clinical orthopedics. With the development of tissue engineering, tissue engineered bone scaffold materials are expected to replace traditional autologous or allogeneic bone, avoiding secondary trauma to patients, and repairing bone defects. Provides a new idea [Dimitriou R, Injury, 2011].

[0003] Biodegradable polyester is a polymer material that can be applied to clinical implants, such as polylactic acid-glycolic acid (PLGA), polylactic acid (PLA), poly...

Examples

Embodiment 1

[0030] Weigh 4.45g of PLGA (Mw=20W), 0.5g of nHA powder, and 0.05g of vancomycin hydrochloride, add them to 10mL of hexafluoroisopropanol, and stir mechanically at room temperature for more than 24 hours to fully dissolve and mix the materials uniformly, and prepare a 3D Print "ink". The 3D printer is 3D-Bioplotter®, put the above "ink" in the barrel of the 3D printer, select the 22# discharge needle, and set the printing parameters: each layer is printed in parallel, the gap is 0.2mm, and the Z-axis direction rises each time 0.2mm, vertical cross-stacking between layers, extrusion speed set at 1mm / s, receiving platform temperature at -10°C, print size as length*width*height=6mm*6mm*4mm cube. After the scaffold was printed, it was freeze-dried for 48 hours, and then placed in a vacuum oven at 60°C. The compressive strength of the material within the range of elastic deformation is 37.95MPa.

[0031] as attached figure 1 In the SEM image of the printed product in this embodi...

Embodiment 2

[0033] Weigh 2.45g of PLGA (Mw=20W), 2.5g of nHA powder, and 0.05g of vancomycin hydrochloride, add it to 10mL of hexafluoroisopropanol, and stir mechanically at room temperature for more than 24 hours to fully dissolve and mix the materials, and prepare a 3D Print "ink". The 3D printer is 3D-Bioplotter®, put the above "ink" in the barrel of the 3D printer, select the 22# discharge needle, and set the printing parameters: each layer is printed in parallel, the gap is 0.2mm, and the Z-axis direction rises each time 0.2mm, vertical cross-stacking between layers, extrusion speed set at 1mm / s, receiving platform temperature at -10°C, print size as length*width*height=6mm*6mm*4mm cube. After the scaffold was printed, it was freeze-dried for 48 hours, and then placed in a vacuum oven at 60°C. The compressive strength within the elastic deformation range of the material is 10.35MPa.

Embodiment 3

[0035] Weigh 3.95g of PLGA (Mw=10W) and PCL (Mw=8W) at a mass ratio of 5:5, 1g of nHA powder, and 0.05g of vancomycin hydrochloride, add it to 10mL of hexafluoroisopropanol, and stir mechanically at room temperature for 24h Above, the materials are fully dissolved and mixed evenly to prepare the 3D printing "ink". The 3D printer is 3D-Bioplotter®, put the above "ink" in the barrel of the 3D printer, select the 22# discharge needle, and set the printing parameters: each layer is printed in parallel, the gap is 0.2mm, and the Z-axis direction rises each time 0.2mm, vertical cross-stacking between layers, extrusion speed set at 1mm / s, receiving platform temperature at -10°C, print size as length*width*height=6mm*6mm*4mm cube. After the scaffold was printed, it was freeze-dried for 48 hours, and then placed in a vacuum oven at 60°C. The compressive strength of the material within the range of elastic deformation is 24.08MPa.

technical field

[0001] The present invention relates to the technical field of biomedical materials, in particular to a method, product and application of a low-temperature 3D printing technology for preparing a drug-loaded polyester polymer / bioceramic bone repair scaffold, using hexafluoroisopropanol as a solvent and adopting low-temperature 3D printing Technology to prepare drug-loaded polyester polymer / bioceramic bone repair scaffold. Background technique

[0002] The repair of large bone defects is a major problem in clinical orthopedics. With the development of tissue engineering, tissue engineered bone scaffold materials are expected to replace traditional autologous or allogeneic bone, avoiding secondary trauma to patients, and repairing bone defects. Provides a new idea [Dimitriou R, Injury, 2011].

[0003] Biodegradable polyester is a polymer material that can be applied to clinical implants, such as polylactic acid-glycolic acid (PLGA), polylactic acid (PLA), poly...

Examples

Embodiment 1

[0030] Weigh 4.45g of PLGA (Mw=20W), 0.5g of nHA powder, and 0.05g of vancomycin hydrochloride, add them to 10mL of hexafluoroisopropanol, and stir mechanically at room temperature for more than 24 hours to fully dissolve and mix the materials uniformly, and prepare a 3D Print "ink". The 3D printer is 3D-Bioplotter®, put the above "ink" in the barrel of the 3D printer, select the 22# discharge needle, and set the printing parameters: each layer is printed in parallel, the gap is 0.2mm, and the Z-axis direction rises each time 0.2mm, vertical cross-stacking between layers, extrusion speed set at 1mm / s, receiving platform temperature at -10°C, print size as length*width*height=6mm*6mm*4mm cube. After the scaffold was printed, it was freeze-dried for 48 hours, and then placed in a vacuum oven at 60°C. The compressive strength of the material within the range of elastic deformation is 37.95MPa.

[0031] as attached figure 1 In the SEM image of the printed product in this embodi...

Embodiment 2

[0033] Weigh 2.45g of PLGA (Mw=20W), 2.5g of nHA powder, and 0.05g of vancomycin hydrochloride, add it to 10mL of hexafluoroisopropanol, and stir mechanically at room temperature for more than 24 hours to fully dissolve and mix the materials, and prepare a 3D Print "ink". The 3D printer is 3D-Bioplotter®, put the above "ink" in the barrel of the 3D printer, select the 22# discharge needle, and set the printing parameters: each layer is printed in parallel, the gap is 0.2mm, and the Z-axis direction rises each time 0.2mm, vertical cross-stacking between layers, extrusion speed set at 1mm / s, receiving platform temperature at -10°C, print size as length*width*height=6mm*6mm*4mm cube. After the scaffold was printed, it was freeze-dried for 48 hours, and then placed in a vacuum oven at 60°C. The compressive strength within the elastic deformation range of the material is 10.35MPa.

Embodiment 3

[0035] Weigh 3.95g of PLGA (Mw=10W) and PCL (Mw=8W) at a mass ratio of 5:5, 1g of nHA powder, and 0.05g of vancomycin hydrochloride, add it to 10mL of hexafluoroisopropanol, and stir mechanically at room temperature for 24h Above, the materials are fully dissolved and mixed evenly to prepare the 3D printing "ink". The 3D printer is 3D-Bioplotter®, put the above "ink" in the barrel of the 3D printer, select the 22# discharge needle, and set the printing parameters: each layer is printed in parallel, the gap is 0.2mm, and the Z-axis direction rises each time 0.2mm, vertical cross-stacking between layers, extrusion speed set at 1mm / s, receiving platform temperature at -10°C, print size as length*width*height=6mm*6mm*4mm cube. After the scaffold was printed, it was freeze-dried for 48 hours, and then placed in a vacuum oven at 60°C. The compressive strength of the material within the range of elastic deformation is 24.08MPa.

Images