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Method of separating and extracting coenzyme Q10 from microbial fermentation broth

A microbial fermentation liquid, solid-liquid separation technology, applied in quinone separation/purification, organic chemistry, etc., to prevent dust from contaminating products, improve extraction yield, and increase the area of ​​reaction contact

Active Publication Date: 2019-03-01
INNER MONGOLIA KINGDOMWAY PHARMA LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polysaccharides are high-molecular carbohydrates, ginsenosides are sterol compounds and triterpenoid saponins, and coenzyme Q10 is a vitamin-like substance and a fat-soluble quinone compound. The ultrafine pulverization technology is applied to the separation and extraction of coenzyme from microbial fermentation broth Q10 has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Get 10L of rhodobacter sphaeroides fermented liquid (content is 3150mg / L), with Buchner funnel suction filter, the bacterial powder is dried in the vacuum oven, the bacterial powder after drying is pulverized to The particle size is about 2 μm, put it in a wide-mouth bottle, add 5 times the mass of n-hexane, stir and extract at about 30 ° C for 1 hour, the extract and bacterial residue are separated by a cyclone separator, the extract is passed through a silica gel column, and 4% of the Wash and elute with ethanol-n-hexane solution, collect the main section for concentration, recycle the solvent, add 2 times the mass of ethanol to the concentrate, heat to 60°C to dissolve, then cool down to 5°C at a rate of 5°C / h to crystallize , centrifuged, and dried to obtain 30.02 g of coenzyme Q10 finished product, with a purity of 99.60% and a total yield of 95.30%.

Embodiment 2

[0048] Get 10L of rhodobacter sphaeroides fermented liquid (content is 3150mg / L), with Buchner funnel suction filter, the bacterial powder is dried in the vacuum oven, the bacterial powder after drying is pulverized to The particle size is about 5 μm, put it in a jar, add 10 times the mass of ether, stir and extract at about 40 ° C for 1.5 hours, the extract and fungal residue are separated by a cyclone separator, the extract is passed through a silica gel column, and 10% Wash and elute with methyl acetate-methanol solution, collect the main section for concentration, recycle the solvent, add 4 times the mass of acetone to the concentrate, heat to dissolve, then cool down to 10°C at a rate of 10°C / h to crystallize, centrifuge Filter and dry to obtain 30.53g of coenzyme Q10 finished product with a purity of 99.61% and a total yield of 96.92%.

Embodiment 3

[0050] Get 10L of rhodobacter sphaeroides fermented liquid (content is 3150mg / L), with Buchner funnel suction filter, the bacterial powder is dried in the vacuum oven, the bacterial powder after drying is pulverized to The particle size is about 10 μm, put it in a jar, add 15 times the quality of petroleum ether, stir and extract at about 35 ° C for 1.2 hours, the extract and fungus residue are passed into a cyclone separator for separation, the extract is passed through a silica gel column, and 15% The butanone-n-heptane solution was washed and eluted, the main section was collected and concentrated, the solvent was recycled, and 8 times the mass of ethanol was added to the concentrate, heated to 55°C to dissolve, and then cooled at a rate of 12°C / h to Crystallized at 15°C, centrifugally filtered, and dried to obtain 30.72 g of finished coenzyme Q10 with a purity of 99.67% and a total yield of 97.52%.

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Abstract

The invention discloses a method of separating and extracting coenzyme Q10 from microbial fermentation broth. The method includes filtering the microbial fermentation broth, and drying to obtain bacterial powder; using a micronization technique to crush the bacterial powder into coenzyme Q10 micropowder 2-35 mu m in particle size; extracting, performing solid-liquid separation, carrying out chromatography, and crystallizing to obtain finished coenzyme Q10. The method herein has high yield; product quality is good; the process is simple; extraction time is short; separation and extraction efficiency is high; the cost is low; the method is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of extraction of coenzyme Q10, in particular to a method for separating and extracting coenzyme Q10 from microbial fermentation broth. Background technique [0002] Coenzyme Q10 (Coenzyme Q10, abbreviated as CoQ10), also known as ubiquinone, is a vitamin-like substance widely present in animals, plants and microorganisms. Coenzyme Q10 is a cell metabolism activator and antioxidant synthesized spontaneously by organisms. It can act on certain enzymes to cause changes in their three-dimensional structures, thereby affecting their physiological activities. Past research and clinical trials have proved that coenzyme Q10 can increase the body's immunity, prevent cardiovascular and cerebrovascular sclerosis, and help improve hypertension, congestive heart failure, nervous system diseases, and tumor treatment. At present, coenzyme Q10, as a precious natural product, is widely used in the production of biochemical drugs, hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C50/28C07C46/10
CPCC07C46/10C07C2601/16C07C50/28
Inventor 胡泽君廖炜程王丽薛文琳石晓梅
Owner INNER MONGOLIA KINGDOMWAY PHARMA LTD
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