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Use of cashew nut shell liquid active substances as tyrosinase inhibitors and plant-derived antibacterial agents

A technology of cashew nut shell oil and active substances, applied in the direction of plant growth regulators, plant growth regulators, chemicals for biological control, etc., can solve the problem of no further detection of plant pathogenic fungi, high cost, no further detection and extraction Purification of antibacterial agents and other issues to achieve the effect of inhibiting mushroom tyrosinase diphenolase activity, simple steps, and simple and fast processing

Inactive Publication Date: 2020-09-18
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese Patent Application No. CN200810147316.8 discloses a preparation method of antibacterial agent of bitter gourd extract, which has better antibacterial effect but higher cost
Chinese patent CN201110126437.6 proposes a method for extracting bacteriostatic agents and acorn extract as a bacteriostatic agent has obvious bacteriostatic effects on Staphylococcus aureus and Escherichia coli, but there is no further improvement on plant pathogenic fungi that have attracted much attention. Detection effect
Invention patents such as Cao Haiqun (application number CN201010265299.5) disclose a botanical pesticide containing cashew nut shell oil and its preparation method and the control effect on aphids, but the raw material component of this invention is cashew nut shell oil stock solution, and there is no Further detection, extraction and purification and use of its active substances in antibacterial agents, there has been no patent disclosure of specific active ingredient components of cashew nut shell oil used as plant-derived antibacterial agents

Method used

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  • Use of cashew nut shell liquid active substances as tyrosinase inhibitors and plant-derived antibacterial agents
  • Use of cashew nut shell liquid active substances as tyrosinase inhibitors and plant-derived antibacterial agents
  • Use of cashew nut shell liquid active substances as tyrosinase inhibitors and plant-derived antibacterial agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Cashew nut shell liquid active material is used as tyrosinase inhibitor and antibacterial agent preparation method

[0022] The active ingredient of the extracted and purified cashew nut shell oil accounts for 95%, and the emulsifier is 5%, and it is stirred for 1 hour to fully mix. That is, the tyrosinase inhibitor and the plant-derived antibacterial agent were obtained and stored at 4°C for future use. When used, dilute with organic solvent methanol or DMSO to prepare the required concentration.

Embodiment 2

[0023] Embodiment 2: Determination of the inhibitory effect of active substances of cashew nut shell oil on tyrosinase diphenolase activity

[0024]The active substance prepared in Example 1 was tested for inhibition of mushroom tyrosinase diphenolase, and the active substance prepared in Example 1 was dissolved in deionized double-distilled water to prepare solutions containing different concentrations of effectors. With 0.5mM L-DOPA as substrate, in 3mL assay system of 0.05M phosphate buffer (pH 6.8), first add 0.1mL of different concentrations of effectors to the cuvette, then add 2.8mL in advance at 30°C Add 0.05mL of mushroom tyrosinase aqueous solution to the substrate solution kept in a constant temperature water bath, mix immediately, measure the optical density value at a wavelength of 475nm with time at a constant temperature of 30°C, and obtain the enzyme enzyme from the slope of the line. Vitality, extinction coefficient according to ε = 3700 (mM cm) -1 calculate....

Embodiment 3

[0026] Example 3: Measuring Analysis of the Mechanism and Type of Cashew Nut Shell Oil Active Substances Inhibiting Acidase

[0027] The cashew nut shell oil active substance prepared in Example 1 was respectively subjected to molecular inhibition mechanism and type analysis tests with tyrosinase. In the assay activity system, the enzyme concentration was 3.33 μg / mL, and the substrate L-DOPA concentration was changed to measure Effects of different concentrations of effectors on the initial velocity of enzyme catalysis, plotting the initial velocity of the enzyme reaction against the substrate concentration is a set of hyperbolas, indicating that the enzymatic reaction follows the Michaelis-Menten kinetic equation. Use the Lineweaver-Burk double reciprocal plot to determine the type of inhibition of the effector. The Michaelis constant K of the enzyme catalyzing the oxidation of L-DOPA was obtained from the intercept of the straight line m and the maximum speed V m . The re...

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Abstract

The invention discloses application of a cashew nut shell oil active substance as a tyrosinase inhibitor and a plant-derived antibacterial agent, and relates to a cashew nut shell oil active substance. The cashew nut shell oil active substance is stirred with an organic solvent; after uniform mixing, the tyrosinase inhibitor and the plant-derived antibacterial agent are obtained. The mass percentage of the cashew nut shell oil active substance to the organic solvent is (90 percent to 95 percent) : (5 percent to 10 percent), the stirring time can be 1 h, and the organic solvent can be selectedfrom one or two of an emulsifier, methanol, isopropanol, dimethyl sulfoxide, acetone, isopropanol, butanol, ethylene glycol, ethyl acetate, acetonitrile and the like. The organic solvent can be used for preparing a single component cardol / cardanol series of the cashew nut shell oil active substance into various concentrations required for detecting a tyrosinase inhibition reaction system. The cashew nut shell oil active substance can be applied to the fields of beauty, fruit preservation, agricultural pathogens prevention and control, and the like, and has a very broad development prospect.

Description

technical field [0001] The invention relates to cashew nut shell oil active substances, in particular to the application of cashew nut shell oil active substances as tyrosinase inhibitors and plant-derived antibacterial agents. Background technique [0002] Cashew nut shell liquid is a by-product of cashew nut planting and processing, with low price and abundant sources. Cashew nut shell contains about 40% cashew shell liquid, which is mainly composed of a mixture of rich phenolic compounds and cashew shell oleoresin. Cashew nut shell oil is a renewable resource with a wide range of sources, and its composition mainly depends on the extraction method. Natural cashew nut shell oil contains about 90% of anacardic acid and 10% of cardol and other active substances. Cashew nut shell liquid has been used in the production of many industrial products. The study found that the active substance of cashew nut shell oil has a special chemical structure, natural unsaturated long-chai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/08A01P21/00A01P3/00
CPCA01N65/00A01N65/08
Inventor 苏伟超陈清西石艳庄江兴王玉霞林玉峰
Owner XIAMEN UNIV